New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA 3363806 =29 (1.180)4 (0.170) 4/272 2.8 13.0% coding (1000/2382 nt) yhcD putative outer membrane fimbrial subunit usher protein
?NC_000913_3_hsa_tpiA 3363821 = 26 (1.110)coding (1015/2382 nt) yhcD putative outer membrane fimbrial subunit usher protein
Rejected: Frequency below/above cutoff threshold.

TCTACCCGCCCTATCGCGAAGCGATA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_hsa_tpiA/3363831‑3363806
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcgataGGGCGGGTAGACGATGACTATGACGAATCACCTTGGGTAATTAGTGCATCGAGCGGATGGAATCTGACACGCTGGAGTGCAATGAACGGCGGCGTTATCGTAGCAGAAAATTATCAGGCGGCATCAATCCGGTCGAGTCTGGTTCCC  >  NC_000913_3_hsa_tpiA/3363821‑3363967
                                                                                                                                                                             
TCTACCCGCCCTATCGCGAAGCGATAGGGCGGGTAGACGATGACTATGACGAATCACCTTGGGTAATTAGTGCATCG                                                                                                  <  1:22956/77‑1
TCTACCCGCCCTATCGCGAAGCGATAGGGCGGGTAGACGATGACTATGACGAATCACCTTGGGTAATTAGTGCATCG                                                                                                  >  2:22956/1‑77
      CGCCCTATCGCGAAGCGATAAGGCGGGTAGACGATGACTATGACGAATCACCTTGCGTAATAAGTGCATCGAGCGGATGGAATCTGACACGCTGGAGTGCAACAAACGGCGGCGTTATCGTACCAAAAAACCATACCGCGGCACCAATC                    >  2:170470/1‑149
                  gAGCGATAGGGCGGGTAGACGATGACTATGACGAATCACCTTGGGTAATTAGTGCATCGAGCGGATGGAATCTGACACGCTGGAGTGCAATGAACGGCCTCTGTATCGTAGCAGAAAATTATCAGGCGGCATCAATCCGGTCGAGTCTG        <  2:180508/148‑1
                      GATAGGGCGGGTAGACGATGACTATGACGAATCACCTTGGGTAATTAGTGCATCGAGCGGATGGAATCTGACACGCTGGAGTGCAATGAACGGCGGCGTTATCGTAGCAGAAAATTATCAGGCGGCATCAATCCGGTCGAGTCTGGTTC    >  2:114604/1‑149
                        TAGGGTGGGTAGACCATTACTATTAAGAATCACCTTGGGTAATTAGTGCATCGAGCGGATGGAATCTGACACGCTGGAGTGCAATGAACGGCGGCGTTATCGTAGCAGAAAATTATCAGGCGGCATCAATCCGGTCGAGTCTGGTTCCC  <  2:398820/149‑1
                                                                                                                                                                             
TCTACCCGCCCTATCGCGAAGCGATA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_hsa_tpiA/3363831‑3363806
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcgataGGGCGGGTAGACGATGACTATGACGAATCACCTTGGGTAATTAGTGCATCGAGCGGATGGAATCTGACACGCTGGAGTGCAATGAACGGCGGCGTTATCGTAGCAGAAAATTATCAGGCGGCATCAATCCGGTCGAGTCTGGTTCCC  >  NC_000913_3_hsa_tpiA/3363821‑3363967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.