New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA 3363806 =29 (1.180)4 (0.170) 4/272 2.8 13.0% coding (1000/2382 nt) yhcD putative outer membrane fimbrial subunit usher protein
?NC_000913_3_hsa_tpiA 3363821 = 26 (1.110)coding (1015/2382 nt) yhcD putative outer membrane fimbrial subunit usher protein
Rejected: Frequency below/above cutoff threshold.

TATCCCTGATGTACCTGTTCGCAATGGTAATAGTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGATGACTATGACGAATCACCTTGGGTAATTAGTGCATCGAGCGGATGGAATCTGACACGCTGGAGTGCAATGAACGGCGGCGTTATCGTAGCAGAAAATTATCAGGCGGCATCAATCCGGTCGAGTCTGGTTCCC  >  NC_000913_3_hsa_tpiA/3363676‑3363967
                                                                                                                                                 |                                                                                                                                                  
tATCCCTGATGTACCTGTTCGCAATGGTAATAGTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGc                                                                                                                                                 <  2:217915/149‑1 (MQ=255)
 gattggtgccgcggtatggttttttggtacgataacgccgccgtttgttgcactccagcgtgtcagattccatccgctcgatgcacttattacgcaaggtgattcgtcatagtcatcgtctacccgcctTATCGCTTCGCGATAGGGCg                                                                                                                                                >  2:170470‑M1/130‑149 (MQ=255)
  tCCCTGATGTACCTGTTCGCAATGGTAATAGTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCgg                                                                                                                                               <  2:223721/149‑1 (MQ=255)
  gcccTGATGTACCTGTTCGCAATGGTAATAGTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCgg                                                                                                                                               <  2:223740/148‑1 (MQ=255)
     cTGTTGAACCTGTTCGCAATGGTAAGAGTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGCTCGCACAACTATATTGCTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTa                                                                                                                                            <  1:388587/149‑1 (MQ=255)
                    gCAATGGTAATAGTGATTTCAAGTCCACCGTGGTAGAACCAGACGCAAGTTCGCACCACTATATTGTTCCCTCCACACTGTTTAATCAGTATGTAGAAAGATTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGATGACTATGAc                                                                                                                             <  2:6148/149‑1 (MQ=255)
                       aTGGTAATAGTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGATGACTATGACGaa                                                                                                                          <  2:254751/149‑1 (MQ=255)
                                 tGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGATGACTATGACGAATCACCTTgg                                                                                                                 <  1:247516/148‑1 (MQ=255)
                                 tGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGATGACTATGACGAATCACCTTgg                                                                                                                 <  1:247636/148‑1 (MQ=255)
                                 tGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGATGACTATGACGAATCACCTTgg                                                                                                                 >  2:247636/1‑148 (MQ=255)
                                 tGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGATGACTATGACGAATCACCTTgg                                                                                                                 >  2:247516/1‑148 (MQ=255)
                                   aTCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGATGACTATGACGAATCACCTTGGGTa                                                                                                              >  1:140152/1‑149 (MQ=255)
                                    tCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGTTAGGGCGGGTAGACGATGACTATGACGAATAACCTTGGGTaa                                                                                                             >  1:398820/1‑149 (MQ=255)
                                           gTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCgg                                                                                                                                               >  1:301661/1‑108 (MQ=255)
                                           gTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCgg                                                                                                                                               <  2:301661/108‑1 (MQ=255)
                                                  ttGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGATGACTATGACGAATCACCTTGGGTAATTAGTGCATCGAGc                                                                                               <  1:344932/149‑1 (MQ=255)
                                                         aaCAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAACGCTTCCAGGGTTCTCGCTTCGCGATAGGGCGGGTAGACGATGACTATGACGAATCCCCTTGGGTAGTTAGTGCACGGAGCGGATggc                                                                                        >  2:204/1‑148 (MQ=255)
                                                                ggTAGTTCACACAACTAGATTCCTTCCTCCAACCTGTTTAAGCAGCATAGAGAAAGCTTCCACGGTTATCGCTTCGCGATAGGGGGGGTAGACGATGACTATGACGAATCGGCTTGGGTAATTAGTGCATCGAGCGGATGGAATCTGac                                                                                 <  2:99182/149‑1 (MQ=255)
                                                                              cTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGATGACTATGACGAATCACCTTGGGTAATTAGGGCATCGAGCGGATGGAATCTGACACGCTGGAGTGCaa                                                                   <  2:92936/149‑1 (MQ=255)
                                                                               cgatgcactaattacccaaggtgattcgtcatagtcatcgtctacccgcccTATCGCTTCGCGATAGGGCGGGTAGa                                                                                                                                          <  1:22956‑M1/26‑1 (MQ=255)
                                                                               cgatgcactaattacccaaggtgattcgtcatagtcatcgtctacccgcccTATCGCTTCGCGATAGGGCGGGTAGa                                                                                                                                          >  2:22956‑M1/52‑77 (MQ=255)
                                                                                      ttCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGATGACTATGACGAATCACCTTGGGTAATTAGTGCATCGAGCGGATGGAATCTGACACGCTGGAGTGCAATGAAcggc                                                           >  1:63060/1‑149 (MQ=255)
                                                                                          cTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGATGACTATGACGAATCACCTTGGGTAATTAGTGCATCGAGCGGATGGAATCTGACACGCTGGAGTGCAATGAACGgcggcg                                                       >  1:61370/1‑149 (MQ=255)
                                                                                                   gTTTAATCAGCATGTAGAAAGCTTCCAGGGTTCTCGCTTCGCGATAGGCCGGGTAGACGATGACTATGACGACTCACCTTGGGGAATTAGTGCATCCGGCGGCTGGAATCGGACACGCTGGAGTGCAAGGAACCGGCGCGTTATCGTAg                                              >  1:112887/1‑149 (MQ=255)
                                                                                                    tttAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGATGACTATGACGAATCACCTTGGGTAATTAGTGCATCGAGCGGATGGAATCTGACACGCTGGAGTGCAATGAACGGCGGCGTTATCGTAGc                                             <  2:119074/149‑1 (MQ=255)
                                                                                                     ttAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGATGACTATGACGAATCACCTTGGGTAATTAGTGCATCGAGCGGATGGAATCTGACACGCTGGAGTGCAATGAACGGCGGCGTTATCGTAGCa                                            >  2:350550/1‑149 (MQ=255)
                                                                                                        aTCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGATGACTATGACGAATCACCGTGGGTAATTAGTGCATCGAGCGGATGGAATCTGACACGCTGGAGTGGAATGAACGGCGGCGTTATCGTAGCAgca                                         >  2:233468/1‑147 (MQ=255)
                                                                                                                    aaaGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGATGACTATGACGAATCACCTTGGGTAATTAGTGCATCGAGCGGATGGAATCTGACACGCTGGAGTGCAATGAACGGCGGCGTTATCGTAGCAGAAAATTATCAggcg                             >  2:351191/1‑149 (MQ=255)
                                                                                                                       tctacccgccctatcgcgaagcgataGGGCGGGTAGACGATGACTATGACGAATCACCTTGGGTAATTAGTGCATCg                                                                                                  >  2:22956‑M2/27‑77 (MQ=255)
                                                                                                                       tctacccgccctatcgcgaagcgataGGGCGGGTAGACGATGACTATGACGAATCACCTTGGGTAATTAGTGCATCg                                                                                                  <  1:22956‑M2/51‑1 (MQ=255)
                                                                                                                             cgccctatcgcgaagcgataAGGCGGGTAGACGATGACTATGACGAATCACCTTGCGTAATAAGTGCATCGAGCGGATGGAATCTGACACGCTGGAGTGCAACAAACGGCGGCGTTATCGTACCAAAAAACCATACCGCGGCACCAATc                    >  2:170470‑M2/21‑149 (MQ=255)
                                                                                                                              gggTTATCGCTGCGCGCTAGGGCGGGTAGACGATGACTATGACGAATCAACTTTGGTAATTAGTGCATCGGGCGGATGGAATCTTACACGCTGGCGTACAATTAACCGCGGCGGTAACGTAGCTGAACATTATCAGCCGGCATCACTcc                   >  2:138323/1‑149 (MQ=255)
                                                                                                                                         gagcgataGGGCGGGTAGACGATGACTATGACGAATCACCTTGGGTAATTAGTGCATCGAGCGGATGGAATCTGACACGCTGGAGTGCAATGAACGGCCTCTGTATCGTAGCAGAAAATTATCAGGCGGCATCAATCCGGTCGAGTCTg        <  2:180508‑M2/141‑1 (MQ=255)
                                                                                                                                             gataGGGCGGGTAGACGATGACTATGACGAATCACCTTGGGTAATTAGTGCATCGAGCGGATGGAATCTGACACGCTGGAGTGCAATGAACGGCGGCGTTATCGTAGCAGAAAATTATCAGGCGGCATCAATCCGGTCGAGTCTGGTTc    >  2:114604‑M2/5‑149 (MQ=255)
                                                                                                                                               taGGGTGGGTAGACCATTACTATTAAGAATCACCTTGGGTAATTAGTGCATCGAGCGGATGGAATCTGACACGCTGGAGTGCAATGAACGGCGGCGTTATCGTAGCAGAAAATTATCAGGCGGCATCAATCCGGTCGAGTCTGGTTccc  <  2:398820‑M2/147‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TATCCCTGATGTACCTGTTCGCAATGGTAATAGTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGATGACTATGACGAATCACCTTGGGTAATTAGTGCATCGAGCGGATGGAATCTGACACGCTGGAGTGCAATGAACGGCGGCGTTATCGTAGCAGAAAATTATCAGGCGGCATCAATCCGGTCGAGTCTGGTTCCC  >  NC_000913_3_hsa_tpiA/3363676‑3363967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.