Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_hsa_tpiA 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_hsa_tpiA 1299499–1300697 1300697 1–1199 55 [0] [0] 56 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA = 12994980 (0.000)55 (1.190) 47/270 0.1 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_hsa_tpiA 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAA  >  NC_000913_3_hsa_tpiA/1300550‑1300846
                                                                                                                                                    |                                                                                                                                                    
ttctcaaagtacagttagttataagtatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaa                                                                                                                                                      <  2:173085‑M2/1‑1 (MQ=255)
     aaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataata                                                                                                                                                 >  1:379911‑M2/144‑149 (MQ=255)
           cagttagttagaaggattaccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgccttttgtagaggaaagtgctaaATAATAATCAAt                                                                                                                                           <  1:290277‑M2/12‑1 (MQ=255)
            agtgagttataatgattgcctgaactgcttctccgcaacatcattttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                          <  2:662665‑M2/13‑1 (MQ=255)
              ttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGt                                                                                                                                        >  2:526367‑M2/135‑149 (MQ=255)
                 gttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaa                                                                                                                                     >  1:67309‑M2/132‑149 (MQ=255)
                 gttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaa                                                                                                                                     >  1:703320‑M2/132‑149 (MQ=255)
                  ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcttttggtagaggaaagtgttaaATAATAATAAATTGTTaaa                                                                                                                                    >  2:285106‑M2/131‑149 (MQ=255)
                    ataaggatttccttaaatgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAatt                                                                                                                                  <  1:163527‑M2/21‑1 (MQ=255)
                       aggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                               <  1:604661‑M2/24‑1 (MQ=255)
                       aggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaatgccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                               <  1:551728‑M2/24‑1 (MQ=255)
                               cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                       >  2:728690‑M2/118‑149 (MQ=255)
                               cttaactgcttctcctcaacatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                       <  1:289312‑M2/32‑1 (MQ=255)
                                ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTc                                                                                                                      >  2:630658‑M2/117‑149 (MQ=255)
                                     tgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttgctagaggaaagtgctaaATAATAATGAATTGTTAAATTATTGTGCATTTCactac                                                                                                                 >  1:724895‑M2/112‑149 (MQ=255)
                                       cttctcctcacaatcatgttattttcgccacatcataatcctgggcttgatgaagaataattgaaatgatcttattaattccactgcctttggtagaggaaagtgataaATAATAATAAATTGTTAAATTATTGTGAATTTCACTACTg                                                                                                               >  2:684045‑M2/110‑149 (MQ=255)
                                          ctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGaa                                                                                                            >  2:306538‑M2/107‑149 (MQ=255)
                                            cctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACt                                                                                                          >  1:813734‑M2/105‑149 (MQ=255)
                                             ctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTg                                                                                                         <  2:703320‑M2/46‑1 (MQ=255)
                                                     catgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGa                                                                                                 >  2:642383‑M2/96‑149 (MQ=255)
                                                      atgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCCCTACTGGAACTGTAATCAGaa                                                                                                >  1:337559‑M2/95‑149 (MQ=255)
                                                      atgttattttccccacatcctaatcctgtgcttgctgcagaataattgaaatgatattattaattccaatgcccttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaa                                                                                                <  1:424489‑M2/55‑1 (MQ=255)
                                                         ttattttcgccacatcataatcctgtgcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGCAAAg                                                                                             >  2:78612‑M2/92‑149 (MQ=255)
                                                              ttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGa                                                                                        <  1:630658‑M2/63‑1 (MQ=255)
                                                               tcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGAc                                                                                       <  1:813086‑M2/64‑1 (MQ=255)
                                                                cgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACa                                                                                      <  2:379911‑M2/65‑1 (MQ=255)
                                                                  ccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATg                                                                                    >  2:621239‑M2/83‑149 (MQ=255)
                                                                          taatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGAATTTCACTACTGGAACTGTAATCAGAAAAGATTGACATGATTCgccc                                                                            >  2:193191‑M2/75‑148 (MQ=255)
                                                                            atcctgggctttctgaagaataattgaaaggatattattaattctactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCttagcgcgt                                                                          <  2:176991‑M2/77‑5 (MQ=255)
                                                                            atcctgggcgtgcggaagaataattgaagttatattattaattccaatggctttggtagaggaaagtgctaaATAATAAGCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAt                                                                          <  2:27382‑M2/77‑1 (MQ=255)
                                                                                tgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATctct                                                                      >  2:466960‑M2/69‑149 (MQ=255)
                                                                                 gggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTa                                                                     <  1:550322‑M2/82‑1 (MQ=255)
                                                                                  ggcttgccgaagaataattgaaatgatattattaattccactgcccttggtagaggaaagtgccaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAt                                                                    <  1:285106‑M2/83‑1 (MQ=255)
                                                                                   gcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAtt                                                                   >  1:292505‑M2/66‑149 (MQ=255)
                                                                                        ctgaagcataattgacatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                              <  1:223243‑M2/89‑1 (MQ=255)
                                                                                        ctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                              <  1:306538‑M2/89‑1 (MQ=255)
                                                                                         tgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaCTAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATATCTATttgattg                                                             >  1:333715‑M2/60‑149 (MQ=255)
                                                                                         tgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGc                                                                                   <  1:366090‑M2/68‑1 (MQ=255)
                                                                                         tgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGc                                                                                   >  2:366090‑M2/60‑127 (MQ=255)
                                                                                             gaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGAAATGACAAGCCGATCTCtatttgattgaggt                                                         >  1:366536‑M2/56‑146 (MQ=255)
                                                                                              aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaattg                                                        >  1:795100‑M2/55‑149 (MQ=255)
                                                                                                   ttgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGa                                                   <  1:621239‑M2/100‑1 (MQ=255)
                                                                                                                attaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGc                                      >  1:142468‑M2/37‑149 (MQ=255)
                                                                                                                        cactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGa                                                                                                 >  2:354228‑M2/29‑82 (MQ=255)
                                                                                                                        cactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGa                                                                                                 <  1:354228‑M2/54‑1 (MQ=255)
                                                                                                                        cactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGc                              >  2:314458‑M2/29‑149 (MQ=255)
                                                                                                                         actgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCa                             >  1:35521‑M2/28‑149 (MQ=255)
                                                                                                                          ctgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCaa                            <  2:292505‑M2/123‑1 (MQ=255)
                                                                                                                          ctgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCaa                            <  1:728690‑M2/123‑1 (MQ=255)
                                                                                                                           tgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATCcaaa                           >  2:347611‑M2/26‑148 (MQ=255)
                                                                                                                               tttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCCTGGATGCAAGCtat                       >  2:647130‑M2/22‑149 (MQ=255)
                                                                                                                               tttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtat                       <  1:642383‑M2/128‑1 (MQ=255)
                                                                                                                               gttagtagaggaaaatgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACGGTAATCAAAAAAGATAGACATGCTTAGCCAATCTCTAGTGGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCtat                       <  2:477081‑M2/128‑1 (MQ=255)
                                                                                                                                    tagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAg                           >  2:570609‑M2/17‑140 (MQ=255)
                                                                                                                                    tagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAg                           <  1:570609‑M2/124‑1 (MQ=255)
                                                                                                                                                    ataataatCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATaa  <  2:514920/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGTCAATAA  >  NC_000913_3_hsa_tpiA/1300550‑1300846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: