New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA = 357087730 (1.260)4 (0.180) 4/274 2.7 11.6% coding (443/1974 nt) glgX glycogen debranching enzyme
?NC_000913_3_hsa_tpiA = 3570891 32 (1.400)coding (429/1974 nt) glgX glycogen debranching enzyme
Rejected: Frequency below/above cutoff threshold.

AGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_hsa_tpiA/3570727‑3570877
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgccccGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGTCAAAGGATTAACGTACTTGCACCCGGAGATCCCGGTCGAGATCCGTGGCACTTATAAAGCCCTCGGGCATCCGGTGATGATCAACTATTTGAAACAATTGGG  <  NC_000913_3_hsa_tpiA/3570891‑3570745
                                                                                                                                                                                                                                                                                                          
AGTTCCAGCGCGGTAATGCGCAATTGTTTCAAATAGTTGATCATTACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCC                                                                                                                                                       <  2:141906/149‑1
                   caAATTGTGTCAAAGAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGG                                                                                                                                    <  2:201987/147‑1
                            TCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGCGGGCACCA                                                                                                                           >  2:419785/1‑149
                                 TAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGGCAGCACCATCATT                                                                                                                      <  1:280010/149‑1
                                       acCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGTGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCCGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAA                                                                                                                <  1:419785/147‑1
                                                                                                                                                   CCCCGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGTCAAAGGATTAACGTACTTGCACCCGGAGATCCCGGTCGAGATCCGTGGCACTTATAAAGCCCTCGGGCATCCAGTGATGATCAACTATTTGAAACAATTG    >  2:251034/1‑149
                                                                                                                                                    CCCGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGTCAAAGGATTAACGTACTTGCACCCGGAGATCCCGGTCGAGATCCGTGGCACTTATAAAGCCCTCGGGCATCCGGTGATGATCAACTATTTGAAACAATTGG   >  1:390067/1‑149
                                                                                                                                                     CCGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGTCAAAGGATTAACGTACTTGCACCCGGAGGTCCCGGTCGAGATCCGTGGCACTTATAAAGCCCTCGGGCATCCGGTGATGATCAACTATTTGAAACACTTGGG  >  2:1222/1‑149
                                                                                                                                                                                                                                                                                                          
AGTTCCAGCGCGGTAATGCCCAATTGTTTCAAATAGTTGATCATCACCGGATGCCCGAGGGCTTTATAAGTGCCACGGATCTCGACCGGGATCTCCGGGTGCAAGTACGTTAATCCTTTGACATGGGCTTCATAAATGATGGTGCTGCCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_hsa_tpiA/3570727‑3570877
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgccccGCCGCGCACGCCGTGGGGCAGCACCATCATTTATGAAGCCCATGTCAAAGGATTAACGTACTTGCACCCGGAGATCCCGGTCGAGATCCGTGGCACTTATAAAGCCCTCGGGCATCCGGTGATGATCAACTATTTGAAACAATTGGG  <  NC_000913_3_hsa_tpiA/3570891‑3570745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.