New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	3190438 =	21 (0.880)	4 (0.180)	4/274	2.7	17.3%	coding (558/750 nt)	yqiH	putative periplasmic pilin chaperone
	?	NC_000913_3_hsa_tpiA	3190453 =	18 (0.790)					coding (573/750 nt)	yqiH	putative periplasmic pilin chaperone
Rejected: Frequency below/above cutoff threshold.

TTCTGGCGGCCAAAAGCCATTGAATTAGAAGACGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGATGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAGCTTAATGGTAACCCCTTTTAGCTATGCAAATACGCAGTTTAAAAATGTTCCGTCAACTTTTCAGGTGGGTTATATCAATGACTTTGGTGGATTAAGTTTTTA  >  NC_000913_3_hsa_tpiA/3190292‑3190566                                                                                                                                                   |                                                                                                                                 aaatgttatttactacagggcagtttatttcataaaaacttaatccaccaaagtcattgatataacccacctgaaaagttgacggaacatttttaaactgcgtatttgcatagctaaaaggggttaccattaagctttgcgtttgtGGt                                                                                                                                <  1:329222‑M1/3‑1 (MQ=255)   atgttatttactacagggcagtttatttcataaaaacttaatccaccaaagtcattgatataacccacctgaaaagttgacggaacatttttaaactgcgaatttgcatagctaaaaggggttaccattaagctttgcgtttgtGGTaa                                                                                                                              <  1:189066‑M1/5‑1 (MQ=255)    tGGCGGCCAAAAGCCATTGAATTAGAAGACGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGATGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAAc                                                                                                                             >  2:282309/1‑149 (MQ=255)      gcggcCAAAAGCCATTGAATTAGAAGACGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGATGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGa                                                                                                                           <  2:386046/149‑1 (MQ=255)       cggcCAAAAGCCATTGAATTAGAAGACGATGAGGGCATGACTTACGAAAAAGTTGAAATCATTTGTAGAAATGACGGGTCGATCCGCTTTAACAATAAGATGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGTGGTAAcatc                                                                                                                          <  1:97077/149‑4 (MQ=255)                        aTTAGAAGACGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGATGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCaaa                                                                                                         <  2:116470/149‑1 (MQ=255)                         ttAGAAGACGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGATGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAg                                                                                                        >  1:391836/1‑149 (MQ=255)                                  gATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACACTAAGCTGCCTTATCACGTTACGCTGGGTTGTATCGGAACAACTGTGGTACCGATGTTGCCACGAACGCAGAGCTTGACGGt                                                                                               >  1:185567/1‑149 (MQ=255)                                  gATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGATGCCTTATCACGTTACGCTGGGTTATACCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAGCTTAATGGt                                                                                               >  1:184799/1‑149 (MQ=255)                                  aaaaacttaatccaccaaagtcattgatataacccacctgaaaagttgacggaacatttttaaactgcgtatttgcatagctaaaaggggttaccattaagctttgcgtttggGGTAACGATGTTAc                                                                                                                     <  2:395928‑M1/14‑1 (MQ=255)                                  aaaaacttaatccaccaaagtcattgatataacccacctgaaaagttgacggaacatttttaaactgcgtatttgcatagctaaaaggggttaccattaagctttgcgtttggGGTAACGATGTTAc                                                                                                                     >  1:395928‑M1/114‑127 (MQ=255)                                    tGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGATGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGtt                                                                                                                       <  2:247376/123‑1 (MQ=255)                                    tGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGATGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGtt                                                                                                                       >  1:247376/1‑123 (MQ=255)                                             taCTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGATGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAGCTTAATGGTAACCCCTTTTa                                                                                    <  1:69748/148‑1 (MQ=255)                                                      aaaaGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGATGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAGCTTAATGGTAACCCCTTTTAGCTTTGCaa                                                                           >  1:146930/1‑149 (MQ=255)                                                      aaaaGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGATGCCTGATAACGTAAAGCTGGGTTATATCGGAACAAATGGGGTAACAATGTAACAACAAACACAAAGCTTAATGTCAACCCCTTTTAGCTATGCaa                                                                           >  2:362087/1‑149 (MQ=255)                                                        attgatataacccacctgaaaagttgacggaacatttttaaactgcgtatttgcatagctaaaaggggttaccattaagctttgcgtttgtGGTAACGATGTTACCACAAACGCAAAGCTTa                                                                                                    >  2:219834‑M1/92‑122 (MQ=255)                                                        attgatataacccacctgaaaagttgacggaacatttttaaactgcgtatttgcatagctaaaaggggttaccattaagctttgcgtttgtGGTAACGATGTTACCACAAACGCAAAGCTTa                                                                                                    <  1:219834‑M1/31‑1 (MQ=255)                                                          gTTGAAATCATTCGTAGAAATGACGGTTCGAACCGCTTTAACAATAAGATGCCTTATCACGTTACGCTGGGGTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAGCTTAATGGTAACCCCTTTTAGCTATGCAAATAc                                                                       <  1:400916/149‑1 (MQ=255)                                                                       gTAGAAATGACGGTTCGATCCGCTTTAACAATAAGATGCCTTATCACGTTACGCTGGGTTATATCGGACCAACTCGGTTTACGATGGTGCTATAAATTCAAAGCTTAATGGTAACCCCTTTTAACTATCCAAATACGCAGTTTCAAAAt                                                          >  2:361656/1‑149 (MQ=255)                                                                                        aTCCGCTTTAACAATAAGATGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAGCTTAATGGTAACCCCTTTTAGCTATGCAAATAGGCAGTTTAAAAATGTTCCGTCAACTTTGCa                                         >  1:419261/1‑149 (MQ=255)                                                                                         tCCGCTTTAACAATAAGATGCCTTATCACGTTACGCTGGGTTATATCGGAACAGATGGGGTAACGATGTTACCACAAACGCAAAGCTTAATGGTAACCCCTTTTAGCTATGCAAATACGCAGTTTAAAAATGTTCCGTCAACTTTTCAg                                        >  1:277546/1‑149 (MQ=255)                                                                                            gCTTTAACAATAAGATGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAGCTTAATGGTAACCCCTTTTAGCTATGCAAATACGCAGTTTAAAAATGTTCCTTCCACTTTTCAGGTg                                     >  1:35048/1‑149 (MQ=255)                                                                                                     aTAAGATGCCTTATCACGTTCCGCCGGGTTATATCGGAACAAATGGGGCCACGACGTTACCACAAACGCAAAGCTTAATGGTAACCCCTTTTAGCTATGCAAATACGCAGTTTAAAAATGTTCCGTCAACTTTTCAGGTGGGTTATATc                            <  1:267869/149‑1 (MQ=255)                                                                                                                 ataaCGTTACGCTGGGTTATATCAGAACACATGGGGTAACGATTTTACTACAAACGTAAAGCTTAATGGTAACCCCGTTTATCTATGCAAATACGCAGTTTAAAAATGTTCCGTCAACTTAGCATTTGGGTTATATCAATGACTTtcgt                <  2:260026/146‑1 (MQ=255)                                                                                                                            cTGGGTTATATCGGAACAAATTGGGTAACGATGTTACCAAAAACGCAAAGCTTAATGGTACCCCCTTTTAGCTATGCAAATACGCAGTTTAAAAATGTTCCGTCAACTTTTCAGGTGGGTTATATCAATGACTTTGGTGGATTAAGttt     <  2:289600/149‑1 (MQ=255)                                                                                                                               ggTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAGCTTAATGGTAACCCCTTTTAGCTATGCAAATACGCAGTTTAAAAATGTTCCGTCAACTTTTCAGGTGGGTTATATCAATGTCTTTCGTGGATTAAGTTTTTa  >  2:234520/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                 TTCTGGCGGCCAAAAGCCATTGAATTAGAAGACGATGGGGTAATGACTTACGAAAAAGTTGAAATCATTCGTAGAAATGACGGTTCGATCCGCTTTAACAATAAGATGCCTTATCACGTTACGCTGGGTTATATCGGAACAAATGGGGTAACGATGTTACCACAAACGCAAAGCTTAATGGTAACCCCTTTTAGCTATGCAAATACGCAGTTTAAAAATGTTCCGTCAACTTTTCAGGTGGGTTATATCAATGACTTTGGTGGATTAAGTTTTTA  >  NC_000913_3_hsa_tpiA/3190292‑3190566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.