New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 311532343 (1.170)4 (0.120) 4/270 4.8 8.9% coding (3790/4563 nt) sslE putative secreted and surface‑associated lipoprotein mucinase
?NC_000913_3_pae_tpiA = 3115328 41 (1.190)coding (3785/4563 nt) sslE putative secreted and surface‑associated lipoprotein mucinase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCAACGGCGTTTCTGCGGCGTTATGACCGACTTCATGCCAGATCAGCCAGTCGTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/3115170‑3115323
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑acatcgttTCACCAACGATGTGCAGATCTCCATCGGTGATGCGCATTCGGGTTATCCGGTAATGAACAGCAGCTTCTCGCCGAACAGCACCACGCTGCCGACGACGCCGCTGAACGACTGGCTGATCTGGCATGAAGTCGGTCATAACGCCGCAG  <  NC_000913_3_pae_tpiA/3115328‑3115182
                                                                                                                                                                                                                                                                                                             
TCAACGGCGTTTCTGCGGCGTTATGACCGACTTCATGCCAGATCAGCCAGTCGTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACA                                                                                                                                                          >  1:636136/1‑149
TCAACGGCGTTTCTGCGGCGTTATGACCGACTTCATGCCAGATCAGCAAGTCGTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACA                                                                                                                                                          >  1:346556/1‑149
   ACGGCGTTTCTGCGGCGTTATGACCGACTTCATGCCAGATCAGCCAGTCGTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCG                                                                                                                                                       >  2:155611/1‑149
                                       AGATCAGCCAGTCGTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTTCACCAACGAT                                                                                                                                          >  1:37508/1‑126
                                       AGATCAGCCAGTCGTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTTCACCAACGAT                                                                                                                                          <  2:37508/126‑1
                                                               TCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTTCACCAACGATGTGCAGATCTCCATCGGTGATGCGCATTCGG                                                                                                           <  1:512323/133‑1
                                                               TCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTTCACCAACGATGTGCAGATCTCCATCGGTGATGCGCATTCGG                                                                                                           >  2:512323/1‑133
                                                                                                                                                        TTTCACCAACGATGTGCAGATCTCCATCGGTGATGCGCATTCGGGTTATCCGGTAATGAACAGCAGCTTCTCGCCGAACAGCACCACGCTGCCGACGACGCCGCTGAACGACTGGCTGATCTGGCATGAAGTCGGTCATAACGCCGCAG  >  1:177522/1‑149
                                                                                                                                                                                                                                                                                                             
TCAACGGCGTTTCTGCGGCGTTATGACCGACTTCATGCCAGATCAGCCAGTCGTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/3115170‑3115323
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑acatcgttTCACCAACGATGTGCAGATCTCCATCGGTGATGCGCATTCGGGTTATCCGGTAATGAACAGCAGCTTCTCGCCGAACAGCACCACGCTGCCGACGACGCCGCTGAACGACTGGCTGATCTGGCATGAAGTCGGTCATAACGCCGCAG  <  NC_000913_3_pae_tpiA/3115328‑3115182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.