New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 3115323	43 (1.170)	4 (0.120)	4/270	4.8	8.9%	coding (3790/4563 nt)	sslE	putative secreted and surface‑associated lipoprotein mucinase
	?	NC_000913_3_pae_tpiA	= 3115328	41 (1.190)					coding (3785/4563 nt)	sslE	putative secreted and surface‑associated lipoprotein mucinase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCGTTTCTGCGGCGTTATGACCGACTTCATGCCAGATCAGCCAGTCGTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGTATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATT  >  NC_000913_3_pae_tpiA/3115176‑3115470                                                                                                                                                    |                                                                                                                                                    gCGTTTCTGCGGCGTTATGACCGACTTCATGCCAGATCAGCCAGTCGTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTg                                                                                                                                                    <  2:636136/149‑1 (MQ=255)   gTTTCTGCGGCGTTATGCCCGACTTCATGCCAGATCAGCCAGTCGTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGt                                                                                                                                                  <  1:43131/149‑1 (MQ=255)       cTGCGGCGTTATGACCGACTTCATGCCAGATCAGCCAGTCGTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGaaa                                                                                                                                              <  1:177522/149‑1 (MQ=255)              gTTATGACCGACTTCATGCCAGATCAGCCAGTCGTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGtt                                                                                                                                       >  1:419719/1‑149 (MQ=255)               ttATGACCGACTTCATGCCAGATCAGCCAGTCGTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGttt                                                                                                                                      <  1:96302/149‑1 (MQ=255)                  tGACCGACTTCATGCCAGATCAGCCAGTCGTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTtgtg                                                                                                                                   >  1:363938/1‑149 (MQ=255)                     ccGACTTCATGCCAGATCAGCCAGTCGTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGCAACGATGTTTGTGGcc                                                                                                                                >  1:403142/1‑149 (MQ=255)                        aCTTCATGCCAGAGCAGCCAGTCGTTCAGCGGCGTCTTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCgg                                                                                                                             <  2:13860/149‑1 (MQ=255)                         cTTCATGCCAGATCAGCCAGTCGTTCCGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGc                                                                                                                            <  1:280284/149‑1 (MQ=255)                          ttCATGCCAGATCAGCCAGTCGTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCa                                                                                                                           >  1:461800/1‑149 (MQ=255)                                  aGATCAGCCAGTCGTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCCCCATAAATACGAGGTTTGTGGCCCGGCAAGTTTTta                                                                                                                   <  2:463750/149‑1 (MQ=255)                                     tCAGCCAGTCGTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATTGGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAg                                                                                                                <  1:413462/149‑1 (MQ=255)                                     gcAGCCAGTCGTGCAGCGGGGGCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCAAATGCGCATCTCCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAg                                                                                                                <  2:576638/148‑1 (MQ=255)                                               gTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATcc                                                                                                      >  1:359843/1‑149 (MQ=255)                                                               ggCAACGTGGTGCTGTTGGGCGAGAAGCTTCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTTTGGCCCGGCATGTTTTTGTAGGTAAAGATGCGGGGTCGACCGTCTTc                                                                                      <  2:221741/149‑1 (MQ=255)                                                                 cAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGc                                                                                    >  1:4555/1‑149 (MQ=255)                                                                     gtggtgCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGGAAACATCCGTTGCTTACCGTCTTCGCTATc                                                                                >  1:623300/1‑149 (MQ=255)                                                                          gCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGc                                                                           >  2:477838/1‑149 (MQ=255)                                                                           cTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTCTTTGTTTCTATGCGGTGCTTACCGTCTTCGCTATCACGGcc                                                                          <  1:155611/149‑1 (MQ=255)                                                                                 ggCGAGAAGCGACTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGGACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGaa                                                                    <  2:467177/149‑1 (MQ=255)                                                                                         gctgctGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGtcattca                                                            >  1:440421/1‑149 (MQ=255)                                                                                           tgcttTTTATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATc                                                          <  1:205285/149‑1 (MQ=255)                                                                                           tgctgTTCATTACCGGATAACCCGAATGCGCATCACCGACGGAGATCTGCACAACGTTGGTGAAACGCTGTTCGTGGCCCGGCAAGTTCTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGAAGACGtcattcacc                                                          >  2:153463/1‑147 (MQ=255)                                                                                             ctgGTCATTACCGGATAACCCGAATGCGCATCACCGAAGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGa                                                        <  1:260053/149‑1 (MQ=255)                                                                                                ttCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTta                                                                                                                   <  2:59321/87‑1 (MQ=255)                                                                                                ttCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTta                                                                                                                   >  1:59321/1‑87 (MQ=255)                                                                                                ttCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCt                                                     >  1:223448/1‑149 (MQ=255)                                                                                                  cATTACCGGATAACCCGAAGGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTgg                                                   <  1:135865/149‑1 (MQ=255)                                                                                                    ttACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCGTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCa                                                 >  1:443424/1‑149 (MQ=255)                                                                                                               cccGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCg                                                                                                     <  2:176251/86‑1 (MQ=255)                                                                                                               cccGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCg                                                                                                     >  1:176251/1‑86 (MQ=255)                                                                                                                ccGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGTATCCAGa                                     >  2:469045/1‑149 (MQ=255)                                                                                                                      gtgcATCAACGACGGAGATCTGCACATCGTGGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGTATCCAGATCGTTa                               <  2:90981/147‑1 (MQ=255)                                                                                                                      gcgcATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGTATCCAGATCGTTa                               <  1:534317/149‑1 (MQ=255)                                                                                                                       gcctccACCGATGGAGATCCACCAATCGTTGGGGAAACGATGTTTGTGGGCGGGCAAGTTTTTATAGGTAAACATCCGGTGTTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGTATCCAGTCCGTTAg                              <  2:403142/144‑1 (MQ=255)                                                                                                                          aTCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGTATCCAGATCGTTAGCGa                           >  1:641221/1‑149 (MQ=255)                                                                                                                          aTCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGATTTTATAGGTAAACATCCGATGCTTACCGTCTTCGCTATCACGGACGTGGCAGCGATGCTGTGAGCTGGCCAAGGTATCCAGAACGTTAGCGa                           >  2:297542/1‑149 (MQ=255)                                                                                                                             aCCGATGGAGATCTGCACATCGTTGGTGAAACGAGGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACAACCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAACTGGCAACGGTATCCAGATCGTTATCGAAtt                        >  2:435129/1‑149 (MQ=255)                                                                                                                                 aTGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGTATCCAGATCGTTAGCGAATTGCTc                    <  1:524387/149‑1 (MQ=255)                                                                                                                                 aTGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGTATCCAGATCGTTACCGAATTGCta                    >  1:104451/1‑148 (MQ=255)                                                                                                                                  gcaggaTCTGCACATCGTTGGTGAACTGAGGTTTGTGGGACGGCCAGTTTTTGTAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGTATCCAGATCGTTAGCGAATTGCTcc                   <  2:550182/145‑1 (MQ=255)                                                                                                                                           gCACATCGTGGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGTATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCa          <  1:332854/149‑1 (MQ=255)                                                                                                                                                   ttGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGTATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAAtt  <  2:27252/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GCGTTTCTGCGGCGTTATGACCGACTTCATGCCAGATCAGCCAGTCGTTCAGCGGCGTCGTCGGCAGCGTGGTGCTGTTCGGCGAGAAGCTGCTGTTCATTACCGGATAACCCGAATGCGCATCACCGATGGAGATCTGCACATCGTTGGTGAAACGATGTTTGTGGCCCGGCAAGTTTTTATAGGTAAACATCCGGTGCTTACCGTCTTCGCTATCACGGCCGTAGAAGTCATTCATCGAGCTGGCAAAGGTATCCAGATCGTTAGCGAATTGCTCCAGTCCGCCAGTGTAATT  >  NC_000913_3_pae_tpiA/3115176‑3115470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.