breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 73 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2450GT64.5% 3.3 / 11.1 35intergenic (‑41/+267)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009133,455,6800GT54.3% 8.2 / 35.7 35V35L (GTG→TTG) gspCType II secretion system protein GspC
*NC_0009132,177,2550GT54.0% ‑0.6 / 35.0 37intergenic (‑51/+257)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_000913454,9260GA50.0% ‑4.9 / 13.8 26intergenic (+137/‑207)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_0009133,455,6770AT48.7% 4.2 / 25.2 39N34Y (AAT→TAT) gspCType II secretion system protein GspC
*NC_000913454,8730CG46.7% 15.1 / 10.2 32intergenic (+84/‑260)bolA/tigDNA‑binding transcriptional dual regulator BolA/trigger factor
*NC_0009133,455,6690TG46.3% 21.8 / 16.4 41I31S (ATT→AGT) gspCType II secretion system protein GspC
*NC_0009133,455,6710AT43.9% 20.1 / 20.9 41I32F (ATT→TTT) gspCType II secretion system protein GspC
*NC_0009134,604,3440CG42.9% 36.9 / 15.6 47S62C (TCT→TGT) bglJDNA‑binding transcriptional regulator BglJ
*NC_0009131,879,8460TG42.4% 24.5 / 15.3 33T35P (ACG→CCG) yeaRDUF1971 domain‑containing protein YeaR
*NC_0009133,407,9710TG42.4% 35.6 / 10.4 33L122R (CTT→CGT) panFpantothenate:Na(+) symporter
*NC_000913767,9560CA41.7% 36.7 / 17.1 36P658Q (CCA→CAA) mngA2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
*NC_0009131,425,5580TG39.1% 54.3 / 14.5 46V61G (GTT→GGT) ynaKRac prophage; ParB‑like nuclease domain‑containing protein YnaK
*NC_0009132,177,2610AT38.5% 52.0 / 15.2 39intergenic (‑57/+251)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I
*NC_0009134,220,0070TG38.5% 21.2 / 19.0 39V471G (GTG→GGG) aceKisocitrate dehydrogenase kinase/phosphatase
*NC_000913361,3050TG37.8% 49.9 / 22.4 45N186H (AAC→CAC) lacAgalactoside O‑acetyltransferase
*NC_0009133,720,4330TC36.0% 23.9 / 10.9 26intergenic (+172/+15)cspA/hokAcold shock protein CspA/small toxic polypeptide
*NC_0009132,594,9520CA35.1% 43.3 / 15.1 37C303F (TGC→TTC) tmcAtRNA(Met) cytidine acetyltransferase
*NC_0009134,220,0160AT35.0% 42.2 / 18.9 40Y474F (TAT→TTT) aceKisocitrate dehydrogenase kinase/phosphatase
*NC_0009132,177,2280CT34.8% 19.0 / 14.4 34intergenic (‑24/+284)gatY/fbaBtagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 454269072 (1.130)12 (0.200) 10/240 3.6 16.7% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 53 (0.890)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 2515617NA (NA)9 (0.150) 9/250 4.1 17.0% noncoding (1345/1345 nt) IS186 repeat region
?NC_000913 2601785 = 44 (0.710)coding (585/618 nt) hyfA hydrogenase 4 component A
* ? NC_000913 4498181 =NA (NA)14 (0.220) 7/258 4.8 NA noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 4499512 NA (NA)intergenic (+12/+81) insD‑6/yjgX KpLE2 phage‑like element; IS2 insertion element protein InsB/KpLE2 phage‑like element; putative phosphoethanolamine transferase YjgX, N‑terminal fragment
* ? NC_000913 381258 =NA (NA)12 (0.190) 7/256 4.8 NA pseudogene (3/417 nt) yaiX putative acyltransferase, N‑terminal fragment
?NC_000913 = 382589 NA (NA)noncoding (1330/1331 nt) IS2 repeat region
* ? NC_000913 = 57448061 (0.950)7 (0.110) 3/258 6.6 10.3% intergenic (+141/+49) ybcQ/nmpC DLP12 prophage; putative antitermination protein/DLP12 prophage; putative outer membrane porin NmpC
?NC_000913 574591 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region