Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,291,610	IS3 (–) +5 bp :: +T	coding (270‑274/1092 nt)	yraK →	putative fimbrial adhesin YraK

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	315229 =	NA (NA)	41 (1.160) +T	31/158	0.2	100%	noncoding (1/1255 nt)	IS3	repeat region
	?	NC_000913	3291610 =	0 (0.000)					coding (270/1092 nt)	yraK	putative fimbrial adhesin YraK
*	?	NC_000913	= 2171428	NA (NA)	44 (1.230)	32/160	0.2	100%	noncoding (1258/1258 nt)	IS3	repeat region
	?	NC_000913	= 3291614	0 (0.000)					coding (274/1092 nt)	yraK	putative fimbrial adhesin YraK

GTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2171350‑2171428 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCTTACCCGCAGAGCAGGAACAGGCGTATTGC  <  NC_000913/3291614‑3291538                                                                                                                                                              GTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAC                                                                              <  2:76135/80‑1       CGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTG                                                                       <  2:110998/81‑1        GGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGG                                                                      >  2:647448/1‑81          CCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGT                                                                     >  2:647461/1‑80           CTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAA                                                                   <  1:111020/81‑1           CTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAA                                                                   <  2:76139/81‑1           CTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAA                                                                   <  2:417458/81‑1           CTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAA                                                                   <  2:110995/81‑1              AGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGT                                                                 <  2:417479/80‑1               GTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTAC                                                               <  2:61196/81‑1               GTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTAC                                                               <  2:417468/81‑1                   GGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGG                                                           <  1:110999/81‑1                   GGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGG                                                           >  1:647523/1‑81                            TGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCAT                                                   <  2:76137/80‑1                             GAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATA                                                  >  1:647520/1‑80                             GAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATA                                                  <  1:417485/80‑1                              AAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATA                                                  >  1:647521/1‑79                              AAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATA                                                  >  1:647522/1‑79                                     GAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGT                                         >  1:647519/1‑81                                      AACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTT                                        >  2:647518/1‑81                                      AACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTT                                        <  1:210150/81‑1                                       ACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTT                                       <  2:76134/81‑1                                             CTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGC                                 >  2:647517/1‑81                                               TAGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCT                               >  1:647514/1‑81                                                AGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCT                               >  1:647516/1‑80                                                AGGCCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCT                               >  2:647515/1‑80                                                   CCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCTTACC                           >  2:647513/1‑81                                                   CCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCTTACC                           >  2:647512/1‑81                                                   CCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCTTACC                           >  2:647511/1‑81                                                   CCTGTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCTTACC                           >  2:647510/1‑81                                                      GTGTCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCTTACCCG                         >  1:647509/1‑80                                                         TCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCTTACCCGCAGA                     <  2:210164/81‑1                                                         TCCATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCTTACCCGCAGA                     <  1:61212/81‑1                                                            ATATTACGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCTTACCCGCAGAG                    >  1:647508/1‑79                                                                  CGTGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCTTACCCGCAGAGCAGGAAC             <  2:111034/80‑1                                                                    TGGGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCTTACCCGCAGAGCAGGAACAGG          <  1:210153/81‑1                                                                      GGTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCTTACCCGCAGAGCAGGAACAGGCG        <  2:417497/81‑1                                                                       GTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCTTACCCGCAGAGCAGGAACAGGCGT       <  1:210152/81‑1                                                                       GTAGGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCTTACCCGCAGAGCAGGAACAGGCGT       <  2:538134/81‑1                                                                          GGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCTTACCCGCAGAGCAGGAACAGGCGTATT    <  1:76136/81‑1                                                                          GGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCTTACCCGCAGAGCAGGAACAGGCGTAT     <  2:61209/80‑1                                                                          GGATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCTTACCCGCAGAGCAGGAACAGGCGTAT     <  2:417480/80‑1                                                                            ATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCTTACCCGCAGAGCAGGAACAGGCGTATTGC  >  1:647506/1‑81                                                                            ATCACAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCTTACCCGCAGAGCAGGAACAGGCGTATTGC  <  1:647507/81‑1                                                                                                                                                              GTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2171350‑2171428 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CAGTGGTGGTAAGTACGGGGCTGACCATATAGACAAGTTTAACTGCCTTACCCGCAGAGCAGGAACAGGCGTATTGC  <  NC_000913/3291614‑3291538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.