mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | ALE-10_S10_L001_R1_001.good.fq | 346,893 | 48,995,034 | 100.0% | 141.2 bases | 149 bases | 93.7% |
| errors | ALE-10_S10_L001_R2_001.good.fq | 346,893 | 48,793,027 | 100.0% | 140.7 bases | 149 bases | 94.3% |
| errors | ALE-10_S10_L002_R1_001.good.fq | 339,245 | 47,823,958 | 100.0% | 141.0 bases | 149 bases | 95.0% |
| errors | ALE-10_S10_L002_R2_001.good.fq | 339,245 | 47,672,203 | 100.0% | 140.5 bases | 149 bases | 94.7% |
| errors | ALE-10_S10_L003_R1_001.good.fq | 354,752 | 50,011,800 | 100.0% | 141.0 bases | 149 bases | 94.9% |
| errors | ALE-10_S10_L003_R2_001.good.fq | 354,752 | 49,828,651 | 100.0% | 140.5 bases | 149 bases | 95.3% |
| errors | ALE-10_S10_L004_R1_001.good.fq | 326,196 | 45,946,565 | 100.0% | 140.9 bases | 149 bases | 94.7% |
| errors | ALE-10_S10_L004_R2_001.good.fq | 326,196 | 45,804,014 | 100.0% | 140.4 bases | 149 bases | 94.4% |
| total | 2,734,172 | 384,875,252 | 100.0% | 140.8 bases | 149 bases | 94.6% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_002947 | 6,181,873 | 59.2 | 2.9 | 100.0% | Pseudomonas putida KT2440 chromosome, complete genome. |
| total | 6,181,873 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 56214 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 629 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.031 |
| reference sequence | pr(no read start) |
|---|---|
| NC_002947 | 0.82561 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.05 |
| Polymorphism minimum variant coverage each strand | 2 |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
| program | version |
|---|---|
| bowtie2 | 2.3.4.1 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 19:23:59 17 Feb 2020 | 19:24:41 17 Feb 2020 | 42 seconds |
| Read alignment to reference genome | 19:24:42 17 Feb 2020 | 19:31:38 17 Feb 2020 | 6 minutes 56 seconds |
| Preprocessing alignments for candidate junction identification | 19:31:38 17 Feb 2020 | 19:32:18 17 Feb 2020 | 40 seconds |
| Preliminary analysis of coverage distribution | 19:32:18 17 Feb 2020 | 19:33:55 17 Feb 2020 | 1 minute 37 seconds |
| Identifying junction candidates | 19:33:55 17 Feb 2020 | 19:42:04 17 Feb 2020 | 8 minutes 9 seconds |
| Re-alignment to junction candidates | 19:42:04 17 Feb 2020 | 19:44:08 17 Feb 2020 | 2 minutes 4 seconds |
| Resolving best read alignments | 19:44:08 17 Feb 2020 | 19:45:08 17 Feb 2020 | 1 minute 0 seconds |
| Creating BAM files | 19:45:08 17 Feb 2020 | 19:46:34 17 Feb 2020 | 1 minute 26 seconds |
| Tabulating error counts | 19:46:34 17 Feb 2020 | 19:47:03 17 Feb 2020 | 29 seconds |
| Re-calibrating base error rates | 19:47:03 17 Feb 2020 | 19:47:06 17 Feb 2020 | 3 seconds |
| Examining read alignment evidence | 19:47:06 17 Feb 2020 | 20:01:55 17 Feb 2020 | 14 minutes 49 seconds |
| Polymorphism statistics | 20:01:55 17 Feb 2020 | 20:01:58 17 Feb 2020 | 3 seconds |
| Output | 20:01:58 17 Feb 2020 | 20:07:18 17 Feb 2020 | 5 minutes 20 seconds |
| Total | 43 minutes 18 seconds | ||
