| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | NC_002947 | 4,980,585 | +GGC | intergenic (‑24/+44) | PP_4387 ← / ← flgE | hypothetical protein/flagellar hook protein FlgE |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_002947 | = 4980585 | 1 (0.020) | 39 (0.700) +GGC |
35/276 | 0.7 | 97.5% | intergenic (‑24/+44) | PP_4387/flgE | hypothetical protein/flagellar hook protein FlgE |
| ? | NC_002947 | 4980586 = | 1 (0.020) | intergenic (‑25/+43) | PP_4387/flgE | hypothetical protein/flagellar hook protein FlgE | |||||
TAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCA‑GCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GG‑‑‑CGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAG > NC_002947/4980452‑4980727 | tAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCA‑GCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcgg > 6:58525‑M1/1‑134 (MQ=255)tAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCA‑GCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggcccctatgcggc > 4:103382‑M1/1‑134 (MQ=255) gacccttcTTTGGCATTGTTAGAACGTGGCGGGGGCA‑GGGGTGGCAGGCAGAAGCAGCACCAGCTAGGGTATCAATGCCAGTTACGACATCCGCTTCTTTCGCGTTAACCTTCACATTCTT‑GGGTCCGCTTTGCGGCCCCAATTAc < 6:150842/139‑1 (MQ=255) aCCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCA‑GCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaa < 8:98096‑M1/147‑26 (MQ=255) aGGCTTTGCCATTGATAGAACGGGGCGGGGGCA‑GCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacac > 1:438‑M1/1‑119 (MQ=255) aGGCTTTGCCATTGATAGAACGGGGCGGGGGCA‑GCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacac > 4:144736‑M1/1‑119 (MQ=255) aGGCTTTGCCATTGATAGAACGGGGCGGGGGCA‑GCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaaca > 6:265137‑M1/1‑119 (MQ=255) gCTTTGCCATTGATAGAACGGGGCGGGGGCACGCGGGGGAAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACCGCCCCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgatttgcggccccaattacaaacac > 1:206526‑M1/1‑118 (MQ=255) cTTTGCCATTGATAGAACGGGGCGGGGGCA‑GCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaa < 3:168442‑M1/148‑33 (MQ=255) cTTTGCCATTGATAGAACGGGGCGGGGGCA‑GCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacacca > 3:176313‑M1/1‑116 (MQ=255) cTTTGCCATTGATAGAACGGGGCGGGGGCA‑GCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacacc > 5:98913‑M1/1‑116 (MQ=255) ttGATAGAACGGGGCGGGGGCA‑GCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaa > 6:119494‑M1/1‑108 (MQ=255) aGAACGGGGCGGGGGCA‑GCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaat > 8:29212‑M1/1‑103 (MQ=255) aaCGGGGCGGGGGCA‑GCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatca > 4:91085‑M1/1‑101 (MQ=255) ggggca‑gcgggggcagGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatc < 3:142383‑M1/148‑57 (MQ=255) ggca‑gcgggggcagGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGCACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatc > 6:131733‑M1/1‑90 (MQ=255) gca‑gcgggggcagGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctga < 8:139934‑M1/148‑60 (MQ=255) ggggcagGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgat < 6:152653‑M1/147‑65 (MQ=255) caggcagAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtc < 5:119494‑M1/147‑69 (MQ=255) cagcaAGGGGAACAAGCCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcatgtaatctgaatgattgtctgcatgatggtgcttt > 8:135975‑M1/1‑63 (MQ=255) gcaAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatg < 3:281686‑M1/123‑63 (MQ=255) gcaAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatg > 4:281686‑M1/1‑61 (MQ=255) ggAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcatgtcatctgaatgatggtctgcatgatggtgctttcagtggaaa < 3:91085‑M1/148‑94 (MQ=255) aaGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggcccccattacaaacaccaaagccaattcaatcatcaggtcatctgaatgatggtctgcctggtggtggtttcagtgggaatggtc > 8:55563‑M1/1‑50 (MQ=255) aGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaaaaccaaagccaatacaatcatcaggtcatctgaatgatggtatgcatgaaggtgctttcagtggaaatggtctttgc > 5:17160‑M1/1‑44 (MQ=255) gTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgaaggtctgcatgatggtgctttgagtggaaatggtcttgggat > 2:123042‑M1/1‑43 (MQ=255) aaCGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTTGGGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcatt > 1:63091‑M1/1‑42 (MQ=255) gACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcc > 3:219638‑M1/1‑38 (MQ=255) aGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgcttagcgggcccaattacaaacaccaaagccaatacaataatcaggtcatgagtatgatggtctgcatgatggtgcgttcagtggaaatggtcttggcattggcctgg > 8:185003‑M1/1‑35 (MQ=255) gCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctgg < 5:265137‑M1/147‑114 (MQ=255) gCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtc > 6:157762‑M1/1‑34 (MQ=255) gCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtc < 5:157762‑M1/132‑99 (MQ=255) cGCTTCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtag > 7:27498‑M1/1‑32 (MQ=255) ttCATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagtt > 5:11881‑M1/1‑29 (MQ=255) cATTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgct > 7:251583‑M1/1‑27 (MQ=255) aTTCGCGTGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttg < 5:58525‑M1/145‑120 (MQ=255) tGAACCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgcc > 5:60282‑M1/1‑19 (MQ=255) aCCTTCACATTCTT‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgcctt < 6:99125‑M1/146‑131 (MQ=255) acaTTCTT‑TGggccgctttgctgacccaattacatacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcagg < 1:182859‑M1/147‑138 (MQ=255) t‑GGggccgctttgcggccccaattacaaacaccaaagccaatacaatcatcaggtcatctgaatgatggtctgcatgatggtgctttcagtggaaatggtcttggcattggcctggtagttgctctgcgccttgatcaggtttaccag > 7:13998‑M1/1‑3 (MQ=255) | TAGCGGCCGGTGACCAGGCTTTGCCATTGATAGAACGGGGCGGGGGCA‑GCGGGGGCAGGCAGAACCAGCACCAGCAAGGGGAACAAGGCCAGTAACGACAGCCGCTTCATTCGCGTGAACCTTCACATTCTT‑GG‑‑‑CGCTTTGCGGCCCCAATTACAAACACCAAAGCCAATACAATCATCAGGTCATCTGAATGATGGTCTGCATGATGGTGCTTTCAGTGGAAATGGTCTTGGCATTGGCCTGGTAGTTGCTCTGCGCCTTGATCAGGTTTACCAG > NC_002947/4980452‑4980727 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |