mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | ALE-8_S8_L001_R1_001.good.fq | 328,109 | 47,263,586 | 100.0% | 144.0 bases | 149 bases | 93.8% |
| errors | ALE-8_S8_L001_R2_001.good.fq | 328,109 | 47,109,731 | 100.0% | 143.6 bases | 149 bases | 94.3% |
| errors | ALE-8_S8_L002_R1_001.good.fq | 319,395 | 45,956,609 | 100.0% | 143.9 bases | 149 bases | 95.1% |
| errors | ALE-8_S8_L002_R2_001.good.fq | 319,395 | 45,834,806 | 100.0% | 143.5 bases | 149 bases | 94.7% |
| errors | ALE-8_S8_L003_R1_001.good.fq | 331,898 | 47,715,385 | 100.0% | 143.8 bases | 149 bases | 95.0% |
| errors | ALE-8_S8_L003_R2_001.good.fq | 331,898 | 47,567,153 | 100.0% | 143.3 bases | 149 bases | 95.3% |
| errors | ALE-8_S8_L004_R1_001.good.fq | 306,586 | 44,062,346 | 100.0% | 143.7 bases | 149 bases | 95.0% |
| errors | ALE-8_S8_L004_R2_001.good.fq | 306,586 | 43,949,390 | 100.0% | 143.4 bases | 149 bases | 94.4% |
| total | 2,571,976 | 369,459,006 | 100.0% | 143.6 bases | 149 bases | 94.7% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_002947 | 6,181,873 | 57.1 | 2.8 | 100.0% | Pseudomonas putida KT2440 chromosome, complete genome. |
| total | 6,181,873 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 56761 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 513 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.025 |
| reference sequence | pr(no read start) |
|---|---|
| NC_002947 | 0.83542 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.05 |
| Polymorphism minimum variant coverage each strand | 2 |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
| program | version |
|---|---|
| bowtie2 | 2.3.4.1 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 07:48:16 18 Feb 2020 | 07:48:54 18 Feb 2020 | 38 seconds |
| Read alignment to reference genome | 07:48:55 18 Feb 2020 | 07:54:38 18 Feb 2020 | 5 minutes 43 seconds |
| Preprocessing alignments for candidate junction identification | 07:54:38 18 Feb 2020 | 07:55:14 18 Feb 2020 | 36 seconds |
| Preliminary analysis of coverage distribution | 07:55:14 18 Feb 2020 | 07:56:43 18 Feb 2020 | 1 minute 29 seconds |
| Identifying junction candidates | 07:56:43 18 Feb 2020 | 08:02:35 18 Feb 2020 | 5 minutes 52 seconds |
| Re-alignment to junction candidates | 08:02:35 18 Feb 2020 | 08:04:23 18 Feb 2020 | 1 minute 48 seconds |
| Resolving best read alignments | 08:04:23 18 Feb 2020 | 08:05:20 18 Feb 2020 | 57 seconds |
| Creating BAM files | 08:05:20 18 Feb 2020 | 08:06:42 18 Feb 2020 | 1 minute 22 seconds |
| Tabulating error counts | 08:06:42 18 Feb 2020 | 08:07:09 18 Feb 2020 | 27 seconds |
| Re-calibrating base error rates | 08:07:09 18 Feb 2020 | 08:07:12 18 Feb 2020 | 3 seconds |
| Examining read alignment evidence | 08:07:12 18 Feb 2020 | 08:21:37 18 Feb 2020 | 14 minutes 25 seconds |
| Polymorphism statistics | 08:21:37 18 Feb 2020 | 08:21:39 18 Feb 2020 | 2 seconds |
| Output | 08:21:39 18 Feb 2020 | 08:25:26 18 Feb 2020 | 3 minutes 47 seconds |
| Total | 37 minutes 9 seconds | ||
