New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 3315571	49 (0.950)	4 (0.080)	4/248	NT	7.8%	coding (588/702 nt)	PP_2915	membrane protein
	?	NC_002947	= 3315596	48 (0.970)					coding (563/702 nt)	PP_2915	membrane protein

TGATCAGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATT  >  NC_002947/3315454‑3315729                                                                                                                                                |                                                                                                                                      tGATCAGCGCGGCAGGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGGGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAgcaaca                                                                                                                                 >  5:139025‑M2/1‑143 (MQ=255)    tCAGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGc                                                                                                                              <  6:239195/149‑1 (MQ=255)      aGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCg                                                                                                                            <  1:257065/149‑1 (MQ=255)      aGCGCGGCATGTAGCCACGCTGCCAGGACCACGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCg                                                                                                                            >  7:146376/1‑149 (MQ=255)            gCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCaa                                                                                                                      <  6:41066/149‑1 (MQ=255)                    ccACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCaa                                                                                                              <  3:100074/149‑1 (MQ=255)                     cACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAAc                                                                                                             >  2:259593/1‑149 (MQ=255)                      aCGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACt                                                                                                            >  8:34872/1‑149 (MQ=255)                        gCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTg                                                                                                           >  8:54812/1‑148 (MQ=255)                            ccAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGTCACAGCTGCCGGCCAGCGCCTGCAGGCCCCGGTGTTGTTGCACCCGGGCGCCGACCCCGGCGCCCACCACCATGCCGGCCAACGCCCCCAACTGCAcc                                                                                                       >  1:82849/1‑149 (MQ=255)                            ccAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCAccc                                                                                                      <  5:293030/149‑1 (MQ=255)                            ccAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCAccc                                                                                                      <  1:70219/149‑1 (MQ=255)                              aGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGc                                                                                                    >  4:24010/1‑149 (MQ=255)                                gACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAgcaac                                                                                                                                  <  6:126837‑M2/117‑6 (MQ=255)                                gACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAgcaac                                                                                                                                  >  5:126837‑M2/1‑112 (MQ=255)                                  ccGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGTGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGc                                                                                                >  6:243738/1‑149 (MQ=255)                                       gAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTg                                                                                           >  3:49092/1‑149 (MQ=255)                                        aaTGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGt                                                                                             >  7:196119/1‑146 (MQ=255)                                        aaTGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGt                                                                                             <  8:196119/146‑1 (MQ=255)                                                 cgATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCgg                                                                                                                           <  8:306314/107‑1 (MQ=255)                                                 cgATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCgg                                                                                                                           >  7:306314/1‑107 (MQ=255)                                                 cgATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCGAGGCACCAACTGCa                                                                                                         <  2:205467/125‑1 (MQ=255)                                                 cgATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCGAGGCACCAACTGCa                                                                                                         >  1:205467/1‑125 (MQ=255)                                                      tCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAGCCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCcag                                                                             >  6:15905/1‑148 (MQ=255)                                                        aGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCcagcag                                                                          <  7:34872/149‑1 (MQ=255)                                                        aGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCcagca                                                                           <  4:49092/148‑1 (MQ=255)                                                         gcgcCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCagcagc                                                                         >  3:132174/1‑149 (MQ=255)                                                         gcgcCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCagcagc                                                                         >  8:297073/1‑149 (MQ=255)                                                            ccAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCaa                                                                      <  7:297073/149‑1 (MQ=255)                                                               gCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGc                                                                   <  3:24010/149‑1 (MQ=255)                                                               gCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGc                                                                   >  4:43489/1‑149 (MQ=255)                                                                 aCGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCaa                                                                      <  4:203359/144‑1 (MQ=255)                                                                 aCGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCaa                                                                      >  3:203359/1‑144 (MQ=255)                                                                   gCAGGCCAGCG‑CACAGCTGCCGGCCAGTGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCa                                                               <  5:243738/149‑1 (MQ=255)                                                                           gcg‑cACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCgg                                                       <  8:146376/149‑1 (MQ=255)                                                                                            cAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAggg                                       >  5:113486/1‑149 (MQ=255)                                                                                            cAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAggg                                       <  4:132174/149‑1 (MQ=255)                                                                                             aGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCACCGCCCCAGGCCGCGGCCGAAACGCGACAGGgc                                      <  4:247424/149‑1 (MQ=255)                                                                                             aGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGgc                                      >  4:71887/1‑149 (MQ=255)                                                                                                 ccTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGccc                                  >  4:197914/1‑149 (MQ=255)                                                                                                 ccTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGccc                                  <  2:302012/149‑1 (MQ=255)                                                                                                  cTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCAc                                                                                                                 <  3:190061/69‑1 (MQ=255)                                                                                                  cTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCAc                                                                                                                 >  4:190061/1‑69 (MQ=255)                                                                                                   tGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGt                                >  2:172494/1‑149 (MQ=255)                                                                                                     cAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGtt                               <  3:43489/148‑1 (MQ=255)                                                                                                          ccAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTaa                         >  8:4579/1‑149 (MQ=255)                                                                                                           cAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAgcaacaacacctgggcctgcaggcgctggccggcagctgtgcgctggcctgcgtgctggcgctgatatcgcggttcattccgcggtcctggcagcgtggctacatgccgcg                        <  7:155050‑M2/149‑112 (MQ=255)                                                                                                            aGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAgcaacaacacctgggcctgcaggcgctggccggcagctgtgcgctggcctgcgtgctggcgctgatatcgcggtt                                                            <  6:9566‑M2/112‑76 (MQ=255)                                                                                                            aGGTGTTCTTGCAACCAGGCGCCGAGCGCGGCGCCCAgcaacaacacctgggcctgcaggcgctggccggcagctgtgcgctggcctgcgtgctggcgctgatatcgcggtt                                                            >  5:9566‑M2/1‑37 (MQ=255)                                                                                                               tgttgtTGCAACCAGGCGCCGAGCGCTGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCTCGTCCGAAACGCGACAGGGCGCCCGTTTCGTTAGTCAg                    <  6:164462/149‑1 (MQ=255)                                                                                                               tgttgtTGCAACCAGGCGCCGAGCGCGGCGCCCAgcaacaacacctgggcctgcaggcgctggccggcagctgtgcgctggcctgcgtgctggcgctgatatcgcggttcattccgcggtcctggcagcgtggctacatgccgcgctga                    <  7:36718‑M2/149‑116 (MQ=255)                                                                                                                 ttgttgCACCCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAgg                   <  5:15905/148‑1 (MQ=255)                                                                                                                   gttgCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGcc                 >  6:2473/1‑148 (MQ=255)                                                                                                                    ttgCAACCAGGCGCCGAGCGCGGCGCCCAGCCACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGTGCCGCTCGCGCAGCGGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCGCGTTACGTCAGTCAGGCCGa               >  8:245936/1‑149 (MQ=255)                                                                                                                       cAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATgg            <  3:71887/149‑1 (MQ=255)                                                                                                                         aCCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGc          <  3:197914/149‑1 (MQ=255)                                                                                                                           cAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAGGTCAGGCCGATGGGCAg        <  7:54812/149‑1 (MQ=255)                                                                                                                                 ccGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCAtt  <  2:82849/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                      TGATCAGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCG‑CACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATT  >  NC_002947/3315454‑3315729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.