New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	2058506 =	48 (0.870)	5 (0.100)	3/242	NT	9.3%	coding (56/525 nt)	PP_1834	hypothetical protein
	?	NC_002947	2058555 =	52 (0.990)					coding (105/525 nt)	PP_1834	hypothetical protein

GGTTTGCCTATCGGCTTCATAGAATCTGAACCTTTGACCGCAATGGCATGCCTGACGAACACTTGGGCCATCAATTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCAACCTGCGTAACCACAAGGGCGATACCCTGCTGATGCTGGCCAGCTACCACGGTCACCACGAGGCGGTG  >  NC_002947/2058358‑2058651                                                                                                                                                     |                                                                                                                                                  gccgttgtcgttggcaatcagcgggtcggcaccttgcgccagcagcaagcgcaccgcctcgtggtgaccgtggtagctggccagcatcagcagggtatcgcccttgtggttacgcaggttggccggcaggccgctggcaagcaggcgcc                                                                                                                                                   <  2:206239‑M1/1‑1 (MQ=255)   tttGCCTATCGGCTTCATAGAATCTGAACCTTTGACCGCAATGGCATGCCTGACGAACACTTGGGCCATCAATTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCg                                                                                                                                                 <  4:170395/149‑1 (MQ=255)       ccTATCGGCTTCATAGAATCTGAACCTTTGACCGCAATGGCATGCCTGACGAACACTTGGGCCATCAATTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCa                                                                                                                                             <  8:41516/149‑1 (MQ=255)        tcgttggcaatcagcgggtcggcaccttgcgccagcagcaagcgcaccgcctcgtggtgaccgtggtagctggccagcatcagcagggtatcgcccttgtggttacgcaggttggccggcaggccgctggcaagcaggcgcCCGAGCAg                                                                                                                                            <  4:59382‑M1/8‑1 (MQ=255)        cTATCGGCTTCATAGAATCTGAACCTTTGCCCGCAATGGCATGCCTGACGAACACTTGGGCCATCAATTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAg                                                                                                                                            >  7:85279/1‑149 (MQ=255)        cTATCGGCTTCATAGAATCTGAACCTTTGACCGCAATGGCATGCCTGACGAACACTTGGGCCATCAATTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGATCGATGACGAAACCGCTGCGTTCGCCGAGCAg                                                                                                                                            <  8:52658/149‑1 (MQ=255)          aTCGGCTTCATAGAATCTGAACCTTTGACCGCAATGGCATGCCTGACGAACACTTGGGCCATCAATTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGgt                                                                                                                                          >  1:207097/1‑149 (MQ=255)             ggCTTCATAGAATCTGAACCTTTGACCGCAATGGCATGCCTGACGAACACTTGGGCCATCAATTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGtt                                                                                                                                       <  1:331002/149‑1 (MQ=255)                    tAGAATCTGAACCTTTGACCGCAATGGCATGCCTGACGAACACTTGGGCCATCAATTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGt                                                                                                                                >  4:62351/1‑149 (MQ=255)                             aaCCTTTGACCGCAATGGCATGCCTGACGAACACTTGGGCCATCAATTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAg                                                                                                                       >  1:26500/1‑149 (MQ=255)                               ccTTTGACCGCAATGGCATGTCTGACGAACACTTGGGCCATCAATTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAggg                                                                                                                     >  2:36573/1‑149 (MQ=255)                                cTTTGACCGCAATGGCATGCCTGACGAACACTTGGGCCATCAATTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGc                                                                                                                    >  5:340184/1‑149 (MQ=255)                                       cGCAATGGCATGCCTGACGAACACTTGGGCCATCAATTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGt                                                                                                                                >  7:132128/1‑130 (MQ=255)                                       cGCAATGGCATGCCTGACGAACACTTGGGCCATCAATTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGt                                                                                                                                <  8:132128/130‑1 (MQ=255)                                         cAATGGCATGCCTGACGAACACTTGGGCCATCAATTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGa                                                                                                            >  2:104746/1‑148 (MQ=255)                                              gCATGCCTGACGAACACTTGGGCCATCAATTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGc                                                                                                       <  5:240105/148‑1 (MQ=255)                                                 gcgcaccgcctcgtggtgaccgtggtagctggccagcatcagcagggtatcgcccttgtggttacgcaggttggccggcaggccgctggcaagcaggcgcCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCgg                                                                                                   >  3:59382‑M1/101‑149 (MQ=255)                                                 gcgcaccgcctcgtggtgaccgtggtacctggccagcatcagccgggtatcgcccttgtggttacgcaggttggccggcaggccgctggcaagcaggcgcCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCgg                                                                                                   >  2:196706‑M1/101‑149 (MQ=255)                                                   gcaccgcctcgtggtgaccgtggtagctggccagcatcagcagggtatcgcccttgtggttacgcaggttggccggcaggccgctggcaagcaggcgcCCGAGc                                                                                                                                              >  3:272496‑M1/99‑104 (MQ=255)                                                   gcaccgcctcgtggtgaccgtggtagctggccagcatcagcagggtatcgcccttgtggttacgcaggttggccggcaggccgctggcaagcaggcgcCCGAGc                                                                                                                                              <  4:272496‑M1/6‑1 (MQ=255)                                                   gcaccgcctcgtggtgaccgtggtagcaggccagcatcagcagggtctcgcccttctggttacgcaggttggccggcaggccgctggcaagcaggcgcCCGAGCAGGTGTTCGAACCTGCCCGCCAGGGCGACGCCGACATGCTCGGgc                                                                                                 >  3:230170‑M1/99‑149 (MQ=255)                                                     tGACGAACACTTGGGCCATCAATTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACACGCCACAATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGTCGACGACGACATGCTCGGgcgc                                                                                               >  5:259181/1‑149 (MQ=255)                                                       aCGAACACTTGGGCCATCAATTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGgcgc                                                                                               <  6:340184/147‑1 (MQ=255)                                                         cctcgtggtgaccgtggtagctggccagcatcagcagggtatcgcccttgtggttacgcaggttggccggcaggccgctggcaagcaggcgcCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCgg                                                                                                   >  8:250931‑M1/93‑141 (MQ=255)                                                         cctcgtggtgaccgtggtagctggccagcatcagcagggtatcgcccttgtggttacgcaggttggccggcaggccgctggcaagcaggcgcCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCgg                                                                                                   <  7:250931‑M1/49‑1 (MQ=255)                                                           ctaattGGGCCATGAATTATGGCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCt                                                                                          <  8:104236/144‑1 (MQ=255)                                                              cTTGGGCCATCAATTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCt                                                                                          >  7:104236/1‑145 (MQ=255)                                                               ttGGGCCATCAATTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGc                                                                                       >  6:225337/1‑147 (MQ=255)                                                                     cATCAATTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCg                                                                                                             <  7:314107/119‑1 (MQ=255)                                                                         aaTTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGcc                                                                           <  5:225337/149‑1 (MQ=255)                                                                         aaTTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCACCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCgg                                                                                 >  7:144302/1‑143 (MQ=255)                                                                         aaTTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCACCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCgg                                                                                 <  8:144302/143‑1 (MQ=255)                                                                             ctggccagcatcagcagggtatcgcccttgtggttacgcaggttggccggcaggccgctggcaagcaggcgcCCGAg                                                                                                                                               <  2:294643‑M1/5‑1 (MQ=255)                                                                             aTGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGc                                                                        <  2:207097/148‑1 (MQ=255)                                                                              tGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCa                                                                      <  1:104746/149‑1 (MQ=255)                                                                                 cAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGc                                                                            >  5:27602/1‑140 (MQ=255)                                                                                 cAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGc                                                                            <  6:27602/140‑1 (MQ=255)                                                                                 cAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCAAGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCAAcc                                                                   >  1:356360/1‑149 (MQ=255)                                                                                    gaggTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCAACCTGc                                                                <  8:85279/149‑1 (MQ=255)                                                                                             tATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGAc                                                                                                           <  6:189316/97‑1 (MQ=255)                                                                                             tATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGAc                                                                                                           >  5:189316/1‑97 (MQ=255)                                                                                             tATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCAACCTGCGTAACcac                                                        >  3:220703/1‑148 (MQ=255)                                                                                                 tCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCAACCTGCGTAACCACAAggg                                                   >  1:206239/1‑149 (MQ=255)                                                                                                           ccGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCAACCTGCGTAACCACAAGGGCGa                                                <  7:215557/142‑1 (MQ=255)                                                                                                           ccGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCAACCTGCGTAACCACAAGGGCGa                                                >  8:215557/1‑142 (MQ=255)                                                                                                           ccGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCAACCTGCGTAACCACAAGGGCGATACCctg                                         <  8:303104/149‑1 (MQ=255)                                                                                                           ccGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCAACCTGCGTAACCACAAGGGCGATACCctg                                         >  7:303104/1‑149 (MQ=255)                                                                                                             gCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCAACCTGCGTAACCACAAGGGCGATACCctgct                                       >  6:51815/1‑149 (MQ=255)                                                                                                             gCACCCCCCACCATGACCGATGAGCAAACGGCGGCGTTCGCGGTGCTGGTGTTCGACGGTCCCCGCCAGGGGGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCATCCTGCGTAACCACAAGGGCGATACCctgct                                       <  2:356360/149‑1 (MQ=255)                                                                                                                 ccGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCAACCTGCGTAACCACAAGGGCGATACCCTGCTGATg                                   >  2:310968/1‑149 (MQ=255)                                                                                                                       ccaTGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCgg                                                                                 <  1:108674/97‑1 (MQ=255)                                                                                                                       ccaTGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCgg                                                                                 >  2:108674/1‑97 (MQ=255)                                                                                                                        caTGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCAACCTGCGTAACCACAAGGGCGATACCCTGCTGATGCTGGc                              >  8:189912/1‑147 (MQ=255)                                                                                                                            aCCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCAACCTGCGTAACCACAAGGGCGATACCCTGCTGATGCTGGCCAGCt                         <  3:62351/148‑1 (MQ=255)                                                                                                                                 tGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCAACCTGCGTAACCACAAGGGCGATACCCTGCTGATGCTGGCCAGCTACCACg                   >  5:212429/1‑149 (MQ=255)                                                                                                                                   aCGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCAACCTGCGTAACCACAAGGGCGATACCCTGCTGATGCTGGCCAGCTACCACGGt                 <  1:310968/149‑1 (MQ=255)                                                                                                                                    cGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCAACCTGCGTAACCACAAGGGCGATACCCTGCTGATGCTGGCCAGCTACCACGGt                 >  4:193719/1‑148 (MQ=255)                                                                                                                                       aaCCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCGAGCGGCCTGCCGGCCAACCTGCGTAACCACAAGGGCGATACCCTGCTGATGCTGGc                              >  6:289581/1‑132 (MQ=255)                                                                                                                                       aaCCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCAACCTGCGTAACCACAAGGGCGATACCCTGCTGATGCTGGc                              <  5:289581/132‑1 (MQ=255)                                                                                                                                                gTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCAACCTGCGTAACCACAAGGGCGATACCCTGCTGATGCTGGCCAGCTACCACGGTCACCACGAGGCgg    <  2:26500/149‑1 (MQ=255)                                                                                                                                                   cGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCAACCTGCGTAACCACAAGGGCGATACCCTGCTGATGCTGGCCAGCTACCACGGTCACCACGAGGCGGTg  <  7:189912/148‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GGTTTGCCTATCGGCTTCATAGAATCTGAACCTTTGACCGCAATGGCATGCCTGACGAACACTTGGGCCATCAATTATGCCAGGAGGTTCCCTATGTCCAGCCAAGCCGCACCCGCCACCATGACCGATGACGAAACCGCTGCGTTCGCCGAGCAGGTGTTCGAACGTGCCCGCCAGGGCGACGCCGACATGCTCGGGCGCCTGCTTGCCAGCGGCCTGCCGGCCAACCTGCGTAACCACAAGGGCGATACCCTGCTGATGCTGGCCAGCTACCACGGTCACCACGAGGCGGTG  >  NC_002947/2058358‑2058651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.