New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	1472574 =	41 (0.740)	3 (0.060)	3/246	NT	6.8%	coding (253/1491 nt)	alg8	glycosyltransferase Alg8
	?	NC_002947	1472611 =	43 (0.810)					coding (216/1491 nt)	alg8	glycosyltransferase Alg8

AGCCGCAGTTGATCGCCTCGCGGATCACCGAGCTGTACACCTGGGCGGTGGTCAGCGCTTCGATACGGAAGCTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCAT  >  NC_002947/1472463‑1472754                                                                                                                                                     |                                                                                                                                                aGCCGCAGTTGATCGCCTCGCGGATCACCGAGCTGTACACCTGGGCGGTGGTCAGCGCTTCGATACGGAAGCTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTac                                                                                                                                                 <  1:87266/149‑1 (MQ=255)     gCAGTTGATCGCCTCGCGGATCACCGAGCTGTACACCTGGGCGGTGGTCAGCGCTTCGATACGGAAGCTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGc                                                                                                                                             >  3:190651/1‑149 (MQ=255)     actcgcagtcgaagcacttcatcttcctggtcggcgcagtcggcatctggcgctactccatgggcgccacccatttcatccgcggcatgatcttcctctacggcgtgTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGc                                                                                                                                             >  3:284726‑M1/108‑149 (MQ=255)         gcagtcgaagcacttcatcttcctggtcggcgcagtcggcatctggcgctactccatgggcgccacccatttcatccgcggcatgatcttcctctacggcgtgTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTa                                                                                                                                         >  7:162756‑M1/104‑149 (MQ=255)             tCGCCTCGCGGATCACCGAGCTGTACACCTGGGCGGTGGTCAGCGCTTCGATACGGAAGCTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTagag                                                                                                                                      >  5:359236/1‑148 (MQ=255)               gCCTCGCGGATCACCGAGCTGTACACCTGGGCGGTGGTCAGCGCTTCGATACGGAAGCTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGa                                                                                                                                    <  3:251047/148‑1 (MQ=255)                 cTCGCGGATCACCGAGCTGTACACCTGGGCGGTGGTCAGCGCTTCGATACGGAAGCTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGa                                                                                                                                 <  8:46292/149‑1 (MQ=255)                  tCGCGGATCACCGAGCTGTACACCTGGGCGGTGGTCAGCGCTTCGATACGGAAGCTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAgg                                                                                                                                     >  1:241923/1‑144 (MQ=255)                                   gTACACCTGGGCGGTGGTCAGCGCTTCGATACGGAAGCTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAAt                                                                                                               >  5:354533/1‑149 (MQ=255)                                    tACACCTGGGCGGTGGTCAGCGCTTCGATACGGAAGCTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGaaa                                                                                                                <  5:351385/147‑1 (MQ=255)                                    tACACCTGGGCGGTGGTCAGCGCTTCGATACGGAAGCTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATg                                                                                                              >  3:81787/1‑149 (MQ=255)                                       acCTGGGCGGTGGTCAGCGCTTCGATACGGAAGCTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGt                                                                                                           >  8:274063/1‑149 (MQ=255)                                         cTGGGCGGTGGTCAGCGCTTCGATACGGAAGCTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATggg                                                                                                            <  1:151673/146‑1 (MQ=255)                                         cTGGGCGGTGGTCAGCGCTTCGATACGGAAGCTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATggg                                                                                                            >  2:151673/1‑146 (MQ=255)                                              cGGTGGTCAGCGCTTCGATACGGAAGCTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGccc                                                                                                    <  3:73083/149‑1 (MQ=255)                                                    tCAGCGCTTCGATACGGAAGCTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAg                                                                                              >  4:63584/1‑149 (MQ=255)                                                     cAGCGCTTCGATACGGAAGCTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAg                                                                                              >  5:352872/1‑148 (MQ=255)                                                               aTACGGAAGCTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGAt                                                                                   >  7:315491/1‑149 (MQ=255)                                                                  cGGAAGCTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGc                                                                                 <  7:274063/148‑1 (MQ=255)                                                                   ggAAGCTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGcc                                                                                <  6:319638/148‑1 (MQ=255)                                                                   ggAAGCTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGcc                                                                                >  5:319638/1‑148 (MQ=255)                                                                        cttgTGACCATCAGGTAGACATTCGAAGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACttc                                                                          <  6:359236/146‑3 (MQ=255)                                                                        cTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTgc                                                                          >  8:171805/1‑149 (MQ=255)                                                                           gTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTgcg                                                                         <  3:63584/147‑1 (MQ=255)                                                                           gTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGcc                                                                       >  2:358495/1‑149 (MQ=255)                                                                                     gttAGCCATGCGACGGGTCGGTGGCATTACCCAGTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCCTGCCGCGGATTAAATGGGTGGCGCCCATGGAGTTGCGCCAGATGCCGACTGCGCCGACCAGGAAg                                                             <  8:102963/147‑1 (MQ=255)                                                                                     ggTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGa                                                                                               >  7:4064/1‑115 (MQ=255)                                                                                     ggTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGa                                                                                               <  8:4064/115‑1 (MQ=255)                                                                                      gTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAgg                                                                >  8:8712/1‑145 (MQ=255)                                                                                          aCATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGaa                                                        <  8:315491/149‑1 (MQ=255)                                                                                            aTGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGt                                                      >  3:222660/1‑149 (MQ=255)                                                                                                 aCGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTc                                                 <  4:222660/149‑1 (MQ=255)                                                                                                    ggTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGAc                                              <  7:8712/149‑1 (MQ=255)                                                                                                       tacggcgtgtacccgtacctgcgccgcaaggtgcagaaaatgggtaCACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGc                                           >  7:162756‑M2/47‑149 (MQ=255)                                                                                                        ggTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGc                                           <  2:241923/148‑1 (MQ=255)                                                                                                        ggTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGc                                           >  2:312751/1‑148 (MQ=255)                                                                                                          tGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGa                                                            <  4:338044/129‑1 (MQ=255)                                                                                                          tGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGa                                                            >  3:338044/1‑129 (MQ=255)                                                                                                           gcgtgtacccgtacctgcgccgcaaggtgcagaaaatgggtaCACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGt                                       >  3:284726‑M2/43‑149 (MQ=255)                                                                                                            gCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCTGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGaa                                                        <  1:151908/131‑1 (MQ=255)                                                                                                            gCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCTGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGaa                                                        >  2:151908/1‑131 (MQ=255)                                                                                                             cATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTc                                      >  2:154627/1‑148 (MQ=255)                                                                                                               ttACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGaa                                   <  1:257428/149‑1 (MQ=255)                                                                                                                 ACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAg                                  >  4:190651‑M1/1‑148 (MQ=255)                                                                                                                  cccgtacctgcgccgcaaggtgcagaaaatgggtaCACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGat                                <  4:284726‑M2/114‑1 (MQ=255)                                                                                                                  CCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGa                                 <  3:273814‑M1/148‑1 (MQ=255)                                                                                                                  CCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTAGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGTAAGATGAAGTGCTTCGACTGCGAGTCGAAGat                                >  4:81787‑M1/1‑149 (MQ=255)                                                                                                                   CCATTTTCTGCACCTTGCGGCGGAGGTACGGGTACTCGCGGTATAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCTTGGAGTTGCGCCAGATGCCGACTGCGCCGAc                                                                    >  7:221104‑M1/1‑112 (MQ=255)                                                                                                                   CCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGata                               <  1:116583‑M1/149‑1 (MQ=255)                                                                                                                    CATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGatat                              <  8:223937‑M1/149‑1 (MQ=255)                                                                                                                            gCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTCGCAGTCGAAgc                                 <  5:131060/138‑2 (MQ=255)                                                                                                                            gCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTCGCAGTCGAAgc                                 >  6:131060/1‑137 (MQ=255)                                                                                                                             cACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTgg                       >  2:200800/1‑147 (MQ=255)                                                                                                                               ccTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGcagg                   <  7:171805/149‑1 (MQ=255)                                                                                                                               ccTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGcagg                   <  1:312751/149‑1 (MQ=255)                                                                                                                                    cggcgCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGcagggccag              <  6:352872/149‑1 (MQ=255)                                                                                                                                               cGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCa   <  4:273814/149‑1 (MQ=255)                                                                                                                                                gggtaCACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCAt  >  5:367009‑M2/6‑149 (MQ=255)                                                                                                                                                  gtaCACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCAGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCAt  >  5:278209‑M2/4‑147 (MQ=255)                                                                                                                                                     |                                                                                                                                                AGCCGCAGTTGATCGCCTCGCGGATCACCGAGCTGTACACCTGGGCGGTGGTCAGCGCTTCGATACGGAAGCTGGTGACCATCAGGTAGACATGCGACGGGTCGGTGGCATTACCCATTTTCTGCACCTTGCGGCGCAGGTACGGGTACACGCCGTAGAGGAAGATCATGCCGCGGATGAAATGGGTGGCGCCCATGGAGTAGCGCCAGATGCCGACTGCGCCGACCAGGAAGATGAAGTGCTTCGACTGCGAGTCGAAGATATCAGCTGGCAGGGCCAGGGCGATCAGCAT  >  NC_002947/1472463‑1472754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.