New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 5178038	37 (0.700)	3 (0.060)	3/236	NT	7.2%	coding (450/486 nt)	PP_4554	hypothetical protein
	?	NC_002947	= 5178060	43 (0.880)					coding (428/486 nt)	PP_4554	hypothetical protein

CTCGGCCAGGTCGAAATCACCCAGCAAGCGGATCAGTGTCGCCAGTATGCGCCGCGACTCCTGCCGGTACACAGCCTCGACTTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCGGTGTAGCGGGTTTTGCCCTGTTCCTCGTCAAAGCTGATCACGGCGGTCATGAAGGCC  >  NC_002947/5177892‑5178182                                                                                                                                                   |                                                                                                                                                 ctcGGCCAGGTCGAAATCACCCAGCAAGCGGATCAGTGTCGCCAGTATGCGCCGCGACTCCTGCCGGTACACAGCCTCGACTTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCg                                                                                                                                                <  4:17208/149‑1 (MQ=255) ctcGGCCAGGTCGAAATCACCCAGCAAGCGGATCAGTGTCGCCAGTATGCGCCGCGACTCCTGCCGGTACACAGCCTCGACTTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCg                                                                                                                                                >  2:17675/1‑149 (MQ=255)   cGGCCAGGTCGAAATCACCCAGCAAGCGGATCAGTGTCGCCAGTATGCGCCGCGACTCCTGCCGGTACACAGCCTCGACTTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAAGCGGt                                                                                                                                              >  2:316153/1‑149 (MQ=255)      ccAGGTCGAAATCACCCAGCAAGCGGATCAGTGTCGCCAGTATGCGCCGCGACTCCTGCCGGTACACAGCCTCGACTTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCa                                                                                                                                           <  2:272954/149‑1 (MQ=255)      ccAGGTCGAAATCACCCAGCAAGCGGATCAGTGTCGCCAGTATGCGCCGCGACTCCTGCCGGTACACAGCCTCGACTTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAAGCGGTCCa                                                                                                                                           <  1:316153/149‑1 (MQ=255)                aTCACCCAGCAAGCGGATCAGTGTCGCCAGTATGCGCCGCGACTCCTGCCGGTACACAGCCTCGACTTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTcc                                                                                                                                 <  4:148204/149‑1 (MQ=255)                        gCAAGCGGATCAGTGTCGCCAGTATGCGCCGCGACTCCTGCCGGTACACAGCCTCGACTTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCa                                                                                                                              <  4:87396/144‑1 (MQ=255)                        gCAAGCGGATCAGTGTCGCCAGTATGCGCCGCGACTCCTGCCGGTACACAGCCTCGACTTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAgagat                                                                                                                         >  3:87396/1‑145 (MQ=255)                              ggATCAGTGTCGCCAGTATGCGCCGCGACTCCTGCCGGTACACAGCCTCGACTTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGgctggggagaaagcctggaccgcctggtgga                                                                                                                   <  4:343809‑M1/149‑32 (MQ=255)                               gATCAGTGTCGCCAGTATGCGCCGCGACTCCTGCCGGTACACAGCCTCGACTTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGaa                                                                                                                  <  4:266905/149‑1 (MQ=255)                                           cAGTATGCGCCGCGACTCCTGCCGGTACACAGCCTCGACTTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCcctg                                                                                                                        <  5:253794‑M2/131‑5 (MQ=255)                                           cAGTATGCGCCGCGACTCCTGCCGGTACACAGCCTCGACTTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCcctg                                                                                                                        >  6:253794‑M2/1‑127 (MQ=255)                                                   gccgcGACTCCTGCCGGTACACAGCCTCGACTTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATttc                                                                                                         >  3:100822/1‑138 (MQ=255)                                                   gccgcGACTCCTGCCGGTACACAGCCTCGACTTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATttc                                                                                                         <  4:100822/138‑1 (MQ=255)                                                       cgACTCCTGCCGGTACACAGCCTCGACTTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCa                                                                                          <  2:73736/149‑1 (MQ=255)                                                           tCCTGCCGGTACACAGCCTCGACTTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGgctggggagaaagcctggaccgcctggtgg                                                                                                                    <  4:236553‑M1/119‑31 (MQ=255)                                                           tCCTGCCGGTACACAGCCTCGACTTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGgctggggagaaagcctggaccgcctggtgg                                                                                                                    >  3:236553‑M1/1‑89 (MQ=255)                                                           tCCTGCCGGTACACAGCCTCGACTTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCg                                                                                      >  5:318828/1‑149 (MQ=255)                                                                    tACACAGCCTCGACTTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTc                                                                             <  1:17675/149‑1 (MQ=255)                                                                          tgcccgactgatggccgcactggcccaggtgcgtgccgaagtcgGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCttt                                                                                                                                     >  7:267555‑M2/45‑87 (MQ=255)                                                                          tgcccgactgatggccgcactggcccaggtgcgtgccgaagtcgGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCttt                                                                                                                                     <  8:267555‑M2/43‑1 (MQ=255)                                                                             ccgactgatggccgcactggcccaggtgcgtgccgaagtcgGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCTCAGCCCtggtgg                                                                                                                    <  3:294853‑M2/60‑1 (MQ=255)                                                                             ccgactgatggccgcactggcccaggtgcgtgccgaagtcgGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCTCAGCCCtggtgg                                                                                                                    >  4:294853‑M2/42‑101 (MQ=255)                                                                             ccgactgatggccgcactggcccaggtgcgtgccgaagtcgGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAg                                                                    >  5:80423‑M2/42‑149 (MQ=255)                                                                                  TTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCCCCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACg                                                               <  2:43245‑M1/149‑1 (MQ=255)                                                                                      gcacgcacCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCg                                                           <  6:318828/149‑1 (MQ=255)                                                                                        actcacCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGAGGTCCAGGCTTTCTCCCCAGCCCTGGTGGACGCCCAGTTCTTCATGGGCCCGGCAGGCGGTGGCAGTCCAGAGCCAGGCACGGGCGgt                                                         >  6:14461/4‑149 (MQ=255)                                                                                        acgcacCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCGgt                                                         <  6:42451/149‑1 (MQ=255)                                                                                            acCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCGGTGTAg                                                     >  3:192469/1‑149 (MQ=255)                                                                                              cTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCGGTGTAGCg                                                   >  4:204146/1‑149 (MQ=255)                                                                                              cTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCAAGGAACGTGCGGTGTAGCg                                                   >  6:260085/1‑149 (MQ=255)                                                                                                 ggCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCCGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCGGTGTAGCgg                                                  <  7:177550/147‑1 (MQ=255)                                                                                                  gCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCGGTGTAGCGGGtt                                               >  1:94614/1‑149 (MQ=255)                                                                                                   ccAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGc                                                                  <  6:160973/129‑1 (MQ=255)                                                                                                    cAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCg                                                           >  8:105548/1‑135 (MQ=255)                                                                                                    cAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCg                                                           <  7:105548/135‑1 (MQ=255)                                                                                                       tgcgtgccgaagtcgGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCGGTGTAGCGGGTTTTGcc                                          <  6:80423‑M2/134‑1 (MQ=255)                                                                                                            ccATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCGGTGTAGCGGGTTTTGCCCTGtt                                     <  3:204146/149‑1 (MQ=255)                                                                                                                  gtcgGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGAGGTGTAGCGGGTTTTGCCCTGTTCCTCGt                               >  5:86807‑M2/5‑149 (MQ=255)                                                                                                                     gGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCGGTGTAGCGGGTTTTGCCCTGTTCCTCGTCa                             >  7:153604‑M2/2‑148 (MQ=255)                                                                                                                      ggCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTg                                                                        <  7:132683/104‑1 (MQ=255)                                                                                                                      ggCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTg                                                                        >  8:132683/1‑104 (MQ=255)                                                                                                                      ggCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCGGTGTAGCGGGTTTTGCCCTGTTCCTCGTCa                             >  1:325121/1‑147 (MQ=255)                                                                                                                        cATCCGCTGGGTCACCACATCCACCAGGCGGTCCTGGCTTTCTCCCCCGCCCTGGTGTAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCGGTGTAGCGGGTTTTGCCCTGTTCCTCGTCAAAGc                         <  8:153604/149‑1 (MQ=255)                                                                                                                         aTCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCAc                                                                >  8:303984/1‑109 (MQ=255)                                                                                                                         aTCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCAc                                                                <  7:303984/109‑1 (MQ=255)                                                                                                                            cGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCGGTGTAGCGGGTTTTGCCCTGTTCCTCGTCAAAGCTGAt                     <  4:163275/149‑1 (MQ=255)                                                                                                                              cTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCGGTGTAGCGGGTTTTGCCCTGTTCCTCGTCAAAGCTGATCa                   <  4:192469/149‑1 (MQ=255)                                                                                                                                 ggTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCGGTGTAGCGGGTTTTGCCCTGTTCCTCGTCAAAGCTGATCAcag                <  2:325121/149‑3 (MQ=255)                                                                                                                                   tCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCACATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCGGTGTAGCGGGTTTTGCCCTGTTCCTCGTCAAAGc                         >  6:44674/1‑138 (MQ=255)                                                                                                                                        accTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCGGTGTAGCGGGTTTTGCCCTg                                       <  2:179401/119‑1 (MQ=255)                                                                                                                                        accTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCAATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCGGTGTAGCGGGTTTTGCCCTg                                       >  1:179401/1‑119 (MQ=255)                                                                                                                                           tCCACCAGGCGGTCCAGGCTTTCTCCCCAGCcctggtggaggtggtgacccagcggatgcccgactgatggccgcactggcccaggtgcgtgccgaagtcgaggctgtgtaccggcaggagtcgcggcgcatactggcgacactgatcc      <  4:343809‑M2/149‑119 (MQ=255)                                                                                                                                           tCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCGGTGTAGCGGGTTTTGCCCTGTTCCTCGTCAAAGCTGATCACGGCGGTCATg        >  6:110032/1‑147 (MQ=255)                                                                                                                                            ccaccaGGCGGTCCAGGCTTTCTCCCCAGCcctggtggaggtggtgacccagcggatgcccgactgatggccgcactggcccaggtgcgtgccgaagtcgaggctgtgtaccggcagga                                   <  4:236553‑M2/119‑90 (MQ=255)                                                                                                                                            ccaccaGGCGGTCCAGGCTTTCTCCCCAGCcctggtggaggtggtgacccagcggatgcccgactgatggccgcactggcccaggtgcgtgccgaagtcgaggctgtgtaccggcagga                                   >  3:236553‑M2/1‑30 (MQ=255)                                                                                                                                             caccaGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTTGCATTAAAGTGCCAGCCACGGGCGGTGTAGCGGGTTTTGCACTGTTCCTCGTCAAAGCTTAAAACGGCGGTCATCAAg     >  4:252338/1‑148 (MQ=255)                                                                                                                                               ccaGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCGGTGTAGCGGGTTTTGCCCTGTTCCTCGTCAAAGCTGATCACGGCGGTCATGAAGGcc  <  1:43245/149‑1 (MQ=255)                                                                                                                                                caGGgctggggagaaagcctggaccgcctggtggaggtggtgacccagcggatgcccgactgatggccgcactggcccaggtgcgtgccgaagtcgaggctgtgtaccggcaggagtcgcggcgcatactg                   >  6:253794‑M1/1‑4 (MQ=255)                                                                                                                                                caGGgctggggagaaagcctggaccgcctggtggaggtggtgacccagcggatgcccgactgatggccgcactggcccaggtgcgtgccgaagtcgaggctgtgtaccggcaggagtcgcggcgcatactg                   <  5:253794‑M1/131‑128 (MQ=255)                                                                                                                                                   |                                                                                                                                                 CTCGGCCAGGTCGAAATCACCCAGCAAGCGGATCAGTGTCGCCAGTATGCGCCGCGACTCCTGCCGGTACACAGCCTCGACTTCGGCACGCACCTGGGCCAGTGCGGCCATCAGTCGGGCATCCGCTGGGTCACCACCTCCACCAGGCGGTCCAGGCTTTCTCCCCAGCCCTGGTGGAAGCCCATTTCTTCATGGGCCCGGCAGTCGGTGGCATTCCAGTGCCAGGCACGGGCGGTGTAGCGGGTTTTGCCCTGTTCCTCGTCAAAGCTGATCACGGCGGTCATGAAGGCC  >  NC_002947/5177892‑5178182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.