Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_002947	5,681,415	+CGGG	intergenic (‑133/+71)	PP_4986 ← / ← PP_4987	hemolysin III family channel protein/putative Chemotaxis protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 5681415	1 (0.020)	39 (0.660) +CGGG	32/276	1.3	97.6%	intergenic (‑133/+71)	PP_4986/PP_4987	hemolysin III family channel protein/putative Chemotaxis protein
	?	NC_002947	5681416 =	1 (0.020)					intergenic (‑134/+70)	PP_4986/PP_4987	hemolysin III family channel protein/putative Chemotaxis protein

CGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTT‑TGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5681273‑5681415 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCTGCTGCGCAGCCCTAT‑‑CGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTC  >  NC_002947/5681416‑5681545                                                                                                                                                 ||||                                                                                                                                     CGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTT‑TGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCAACTGCATGAACCGCCGGGGC                                                                                                                                    >  4:57466/1‑149  GTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTT‑TGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGG                                                                                                                                       >  7:185306/1‑145  GTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTT‑TGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGG                                                                                                                                       <  8:185306/145‑1       ACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTT‑TGTGGGAGCGGCCTTGTGTAGAGAAAGGGGCGCCAAGCGGCCCCCGGCTTTCAATGCCACAGCATGAACCGCCCGGGCAGCAGC                                                                                                                              >  8:267094/1‑149        CATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGGGATGCTGATGGCTTCGTTCTT‑TGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGC                                                                                                                              <  2:85548/148‑1         ATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTT‑TGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGC                                                                                                                            >  7:261667/1‑149          TGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTT‑TGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCA                                                                                                                           <  5:206801/149‑1             AAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTT‑TGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTGATTTCCACTGCATGAACCGCCGGGGCTGCTGCGCCGCC                                                                                                                        >  5:126466/1‑149             AAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTT‑TGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCC                                                                                                                        >  7:202561/1‑149              AACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTT‑TGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCAAGAACCGCCGGGGCTGCTGCGCAGCC                                                                                                                        >  8:242478/1‑148                 ACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTT‑TGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCGCAGCAGCCCc                                                                                                                      >  2:347787/1‑146                 ACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTT‑TGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCGCAGCAGCCCc                                                                                                                      <  1:347787/147‑2                      CCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTT‑TGTGGGAGCGGCCTTGTGTCGCGAATGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCTGGGCTGCTGCGCAGCCCTAT‑‑CGCGA                                                                                                             <  7:169257/149‑1                       CTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTTGTGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCGCAGCAGCCCcggcggt                                                                                                                >  1:83950/1‑141                            GAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTT‑TGTGGGAGCGGCCGTGTGTCGCGAAACGGGCGCGAAGCGGGCCCAGGCTTTAATAGCCACAGCATGAACCGCCGGCGCTGCTAGGCAGCACCAT‑‑GGCGgcgcacg                                                                                                       >  2:355355/1‑142                                                  TGATGCTGATGGCTTCGTTCTT‑TGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCCGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTAT‑‑CGCGACACAAGGCCGCTCCCACTCCAGACCACC                                                                                 >  6:74918/1‑149                                                               TTCGTTCTT‑TGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCCGCCCTAT‑‑AGCCACAAAAGGCCGCTCCCACACCAGACCACCAGGCCCAGCGCC                                                                     >  2:75889/1‑148                                                               TTCGTTCTT‑TGTGGGAGCGGCCTTGTGTAGCGAAAGGGGCGCGAAGCGGCCCCACGCTTTAATTTCCCCTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTAT‑‑AGCGACACAACGCCCCACCCACAACAGACCACCACGCCCAGCGCCg                                                                    >  4:285382/1‑148                                                                      TT‑TGGGGCAGCGGCCTTGTGGCGCTAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATTAACCCCCGGGGCTGCTGCGCAGCCCTAT‑‑CGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATC                                                              <  3:288234/148‑1                                                                         TGTGGGAGCGGCGTTGTGTCGCGAACGGGGCGCGACGCGGCCCCAGGGTTTCATTGCCACTGCATGAACCGCCGGGGGTGCGGCGCAGCCCTAT‑‑CCCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATC                                                              <  7:324261/146‑1                                                                            GGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTAT‑‑AGCGCCACCAGGCCGGGGCCACTCCAGACCACCCGGCCCTGCGCCGCACCTACTAAC                                                         >  7:210969/1‑148                                                                                    CCTTGTGTCGCTAATGGGGCGCTAAGCGGCCCAAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTAT‑‑CGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAG                                                <  3:285382/149‑1                                                                                    CCTTGTGTCGCGAATGGGGCGCTAAGCGGCCCCAGGCTTTAATTCCCACTGAATTAACCGCCGGGGCTGCTGCGCAGCCCTAT‑‑CGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAG                                                <  1:75889/149‑1                                                                                     gtgtgggCGCGAAAGGGGCGCGAAGCGGCCCCTGGATTTCATTGCCACTGAATTAACCGCCGGGGCTGCTGCGCAGCCCTAT‑‑CGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGG                                               <  7:242478/142‑1                                                                                     CTTGTGTCGCGAAAGGGGCGCGTAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTATCCCTCTAAAAAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCA                                                 <  1:285773/149‑1                                                                                           TCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTAT‑‑CGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTG                                            >  3:253021/1‑146                                                                                               GAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTAT‑‑CGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGC                                                                        <  8:318164/114‑1                                                                                               GAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTAT‑‑CGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGC                                                                        >  7:318164/1‑114                                                                                                        GCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCACTAA‑‑CGCGACACAAGGCCGCTACCCCTCCACACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTAAGGTTTCAGATCCCCGTacacc                            >  8:264418/1‑144                                                                                                          GAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTAT‑‑CGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCAC                          <  4:253021/149‑1                                                                                                             GCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTAT‑‑CGCGACACAAGGCCGCTCACACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTAAGGTTGCAGATCCGCGTCACGCACCAG                       >  2:75329/1‑149                                                                                                                 CCCCAGGCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTAT‑‑CGCGACACAAGGCCGCTCCCACTCCAGACCACCCCGCCCTGCGCCTCAAATACAAACCAAGGTCAGTTAGCAGATCCGCCTGCAGCACCACCCTT                   >  8:62605/1‑149                                                                                                                       GCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTAT‑‑CGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAAT             <  7:117166/149‑1                                                                                                                       GCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTAT‑‑CGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGT                                                   <  6:361262/111‑1                                                                                                                       GCTTTCATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTAT‑‑CGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGT                                                   >  5:361262/1‑111                                                                                                                             ATTGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTAT‑‑CGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGAC        >  7:213860/1‑148                                                                                                                              tgGCCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCATAT‑‑CGCTACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCA      <  3:57466/147‑1                                                                                                                                 CCACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTAT‑‑CGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGT   <  7:267094/149‑1                                                                                                                                  CACTGCATGAACCGCCGGGGCTGCTTCGCAGCCCTAT‑‑CGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTC  <  7:264418/149‑1                                                                                                                                  CACTGCATGAACCGCCGGGGCTGCTGCGCAGCCCTAT‑‑CGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTC  <  8:213860/149‑1                                                                                                                                             CCGCCGGGGCTGCTGCGCAGCCCTAT‑‑CGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGATGCAGATCCGCGTCACGCACCAGCCTTT                  <  1:203996/122‑1                                                                                                                                                 ||||                                                                                                                                     CGTAATACATGCAAACACAGACCTCCTGAGGTGGGCTGGGTTCCTGTTGTGATGCTGATGGCTTCGTTCTT‑TGTGGGAGCGGCCTTGTGTCGCGAAAGGGGCGCGAAGCGGCCCCAGGCTTTCATTGCCACTGCATGAACCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5681273‑5681415 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCTGCTGCGCAGCCCTAT‑‑CGCGACACAAGGCCGCTCCCACTCCAGACCACCAGGCCCTGCGCCTCACATCCTAACCAAGGTCAGGTTGCAGATCCGCGTCACGCACCAGCCTTTCCAATGCGACCAGGTC  >  NC_002947/5681416‑5681545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.