New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 4553242	57 (0.920)	3 (0.050)	3/260	NT	5.4%	coding (1684/1797 nt)	PP_4041	glycoside hydrolase
	?	NC_002947	= 4553296	50 (0.830)					coding (1630/1797 nt)	PP_4041	glycoside hydrolase

GCGCGCCGGGCACTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑GCCCTCTCCCGGCGTCAGGCCGTCGCTGCAGCTGTCACTGTCATAGC  >  NC_002947/4553150‑4553430                                                                                                                                                   |                                                                                                                                        gcgcgcCGGGCACTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGt                                                                                                                                         >  3:22940/1‑147 (MQ=255)      ccGGGCACTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGgtgt                                                                                                                                  >  5:262693/1‑149 (MQ=255)        gggCACTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTg                                                                                                                                <  3:313725/149‑1 (MQ=255)         ggCACTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTcag                                                                                                                                      <  7:263315‑M2/142‑4 (MQ=255)          gCACTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGt                                                                                                                              <  2:343355/149‑1 (MQ=255)             cTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACa                                                                                                                           <  4:119353/149‑1 (MQ=255)                tgCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAg                                                                                                                          <  4:158591/147‑1 (MQ=255)                         cAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCtt                                                                                                               <  6:45634/149‑1 (MQ=255)                         cAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCtt                                                                                                               <  3:63559/149‑1 (MQ=255)                           gATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCGGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTg                                                                                                             >  4:379488/1‑149 (MQ=255)                           gATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTg                                                                                                             <  4:22940/149‑1 (MQ=255)                            aTTCAGCGCGGTGTTGATGATGCCTTTCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTgg                                                                                                            <  7:285295/149‑1 (MQ=255)                             ttCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTgg                                                                                                            <  5:281301/148‑1 (MQ=255)                                      gtgtTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGccc                                                                                                  <  6:318831/149‑1 (MQ=255)                                            ataatgCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTa                                                                                             <  3:374458/145‑1 (MQ=255)                                             tgatgCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAac                                                                                           <  2:324872/149‑1 (MQ=255)                                                 gCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCAGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGACGGTCAGGTCTTGGTACAGCGCTTCGGCTTTGGACTGGCGCCCCAGTAAcaca                                                                                        >  8:333205/1‑148 (MQ=255)                                                          gCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTca                                                                               <  7:187695/148‑1 (MQ=255)                                                            tGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGccc                                                                                                  >  5:162101/1‑127 (MQ=255)                                                            tGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGccc                                                                                                  <  6:162101/127‑1 (MQ=255)                                                            tGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTcagc                                                                             >  2:210364/1‑148 (MQ=255)                                                            tGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTc                                                                                <  6:316595/145‑1 (MQ=255)                                                            tGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTc                                                                                >  5:316595/1‑145 (MQ=255)                                                              aaGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAaca                                                                                          >  6:15350/1‑133 (MQ=255)                                                              aaGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTcagcca                                                                           <  6:262693/148‑1 (MQ=255)                                                                   cTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGccagccag                                                                      >  8:317180/1‑148 (MQ=255)                                                                      ggggAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCTGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAgaac                                                                   <  3:379488/148‑1 (MQ=255)                                                                         gAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGATCGTTGCACAGGCCGGTCAGGTGTAGGTACAGCGCTTCCGCTTTGGCCTGGCGCCCCAGTAACACATAAACCTCAGCCAGCCAgaacgaac                                                               >  6:248148/1‑149 (MQ=255)                                                                                  gAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAgg                                                       >  6:168710/1‑148 (MQ=255)                                                                                        gcgcTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCAAGCCAGAACGAACACACCAGGAAGGT‑Gc                                               >  5:163074/1‑149 (MQ=255)                                                                                         cgcTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑Gc                                               <  6:166924/148‑1 (MQ=255)                                                                                          gcTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTcagcgcatgctcggcaacttcccccaggccttcagccagataattgggacatcaacaccgcgctgaatctgcaccgcgcacagtgcccggc                                              <  1:131105‑M2/149‑92 (MQ=255)                                                                                          gcTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑Gccc                                             >  5:42721/1‑149 (MQ=255)                                                                                          gcTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑Gccc                                             >  2:269803/1‑149 (MQ=255)                                                                                            tGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTcagcgcatgctcggcaacttcccccaggccttcagccagataggcatcatcaacaccgcgctgaatctgcaccgcgcacagtgcccggcgcgc                                            <  1:150427‑M2/149‑94 (MQ=255)                                                                                                 ggccggGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑GCCCTCTCCCg                                      >  2:107135/1‑149 (MQ=255)                                                                                                   ccggGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑GCCCTCTCCCGGc                                    <  6:42721/149‑1 (MQ=255)                                                                                                   ccggGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑GCCCTCTCCCGGc                                    <  7:317180/149‑1 (MQ=255)                                                                                                    cggGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTcagcgcatgctcggcaacttcccccaggccttcagccagataggcatcatcaacaccgcgctgaatctgcaccgcgcacagtgcccggcgcgcgaccgggc                                    >  2:150427‑M2/1‑48 (MQ=255)                                                                                                           tACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑GCCCTCTCCCGGCGTCAGGcc                            >  6:207989/1‑149 (MQ=255)                                                                                                           tACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑GCCCTCTCCCGGCGTCAGGcc                            >  1:327480/1‑149 (MQ=255)                                                                                                           tACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑GCCCTCTCCCGGCGTCAGGcc                            >  7:4819/1‑149 (MQ=255)                                                                                                               gTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑GCCCTCTCCCGGCGTCAGGCCGTCg                        >  7:162616/1‑149 (MQ=255)                                                                                                               cctCGGCCTGCTGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑GCCCTCTCCCGGCGTCAGGCCGTCg                        >  4:360302/3‑149 (MQ=255)                                                                                                                ttCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAACGT‑GCCCTCTCCCGGCGTCAGCCCGTCg                        >  7:336610/1‑148 (MQ=255)                                                                                                                   ggCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑GCCCTCTCCCGGCGTc                                 <  2:337219/136‑1 (MQ=255)                                                                                                                   ggCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑GCCCTCTCCCGGCGTc                                 >  1:342713/1‑136 (MQ=255)                                                                                                                   ggCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑GCCCTCTCCCGGCGTc                                 >  1:337219/1‑136 (MQ=255)                                                                                                                    ggcagcagGCCGAGGTCGTTGCACAGTCCTGTGAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑GCCCTCTCCCGGCGTCAGGCCGTCGCTGCa                   <  8:146250/147‑1 (MQ=255)                                                                                                                      cagcagGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑GCCCTCTCCCGGCGTCAGGCCGTCGCTGCAGc                 >  7:51390/1‑149 (MQ=255)                                                                                                                            gCCGAGGTCGTTGCACAGGCCGGTCAGCTGTAGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑GCCCTCTCCCGGCGTCACGCCGTCGCTGCAGcggtcac           >  8:98456/1‑149 (MQ=255)                                                                                                                             ccGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑GCCCTCTCCCGGCGTCAGGCCGTCGCTGCAGctgtcact          <  1:210364/149‑1 (MQ=255)                                                                                                                              cGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑GCCCTCTCCCGGCGTCAGGCCGTCGCTGCAGctgtcactg         <  2:327480/149‑1 (MQ=255)                                                                                                                              cGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑GCCCTCTCCCGGCGTCAGGCCGTCGCTGCAGctgtcactg         >  6:224999/1‑149 (MQ=255)                                                                                                                                  gTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGTCGCCCTCTCCCGGCGTCAGGCCGTCGCTGCAGctgtcactgtca      <  1:269803/149‑1 (MQ=255)                                                                                                                                     gTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑GCCCTCTCCCGGCGTCAGGCCGTCGCTGCAGCTGTCACTGTCATAgc  >  6:309527/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                        GCGCGCCGGGCACTGTGCGCGGTGCAGATTCAGCGCGGTGTTGATGATGCCTATCTGGCTGAAGGCCTGGGGGAAGTTGCCGAGCATGCGCTGGCCGGCCGGGTCGTACTGTTCGGCCAGCAGGCCGAGGTCGTTGCACAGGCCGGTCAGGTGTTGGTACAGCGCTTCGGCTTTGGCCTGGCGCCCCAGTAACACATAAACGTCAGCCAGCCAGAACGAACACACCAGGAAGGT‑GCCCTCTCCCGGCGTCAGGCCGTCGCTGCAGCTGTCACTGTCATAGC  >  NC_002947/4553150‑4553430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.