mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | ALE-1_S1_L001_R1_001.good.fq | 271,413 | 38,156,867 | 100.0% | 140.6 bases | 149 bases | 93.9% |
| errors | ALE-1_S1_L001_R2_001.good.fq | 271,413 | 37,986,153 | 100.0% | 140.0 bases | 149 bases | 94.6% |
| errors | ALE-1_S1_L002_R1_001.good.fq | 265,439 | 37,244,025 | 100.0% | 140.3 bases | 149 bases | 95.1% |
| errors | ALE-1_S1_L002_R2_001.good.fq | 265,439 | 37,105,564 | 100.0% | 139.8 bases | 149 bases | 95.1% |
| errors | ALE-1_S1_L003_R1_001.good.fq | 277,654 | 38,923,366 | 100.0% | 140.2 bases | 149 bases | 95.0% |
| errors | ALE-1_S1_L003_R2_001.good.fq | 277,654 | 38,763,307 | 100.0% | 139.6 bases | 149 bases | 95.8% |
| errors | ALE-1_S1_L004_R1_001.good.fq | 254,478 | 35,690,116 | 100.0% | 140.2 bases | 149 bases | 94.9% |
| errors | ALE-1_S1_L004_R2_001.good.fq | 254,478 | 35,561,182 | 100.0% | 139.7 bases | 149 bases | 94.9% |
| total | 2,137,968 | 299,430,580 | 100.0% | 140.1 bases | 149 bases | 94.9% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_002947 | 6,181,873 | 46.1 | 2.5 | 100.0% | Pseudomonas putida KT2440 chromosome, complete genome. |
| total | 6,181,873 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 40222 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 520 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.026 |
| reference sequence | pr(no read start) |
|---|---|
| NC_002947 | 0.85850 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.05 |
| Polymorphism minimum variant coverage each strand | 2 |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
| program | version |
|---|---|
| bowtie2 | 2.3.4.1 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 11:58:49 18 Feb 2020 | 11:59:22 18 Feb 2020 | 33 seconds |
| Read alignment to reference genome | 11:59:22 18 Feb 2020 | 12:04:02 18 Feb 2020 | 4 minutes 40 seconds |
| Preprocessing alignments for candidate junction identification | 12:04:02 18 Feb 2020 | 12:04:32 18 Feb 2020 | 30 seconds |
| Preliminary analysis of coverage distribution | 12:04:32 18 Feb 2020 | 12:05:36 18 Feb 2020 | 1 minute 4 seconds |
| Identifying junction candidates | 12:05:36 18 Feb 2020 | 12:08:36 18 Feb 2020 | 3 minutes 0 seconds |
| Re-alignment to junction candidates | 12:08:36 18 Feb 2020 | 12:10:04 18 Feb 2020 | 1 minute 28 seconds |
| Resolving best read alignments | 12:10:04 18 Feb 2020 | 12:10:50 18 Feb 2020 | 46 seconds |
| Creating BAM files | 12:10:50 18 Feb 2020 | 12:11:57 18 Feb 2020 | 1 minute 7 seconds |
| Tabulating error counts | 12:11:57 18 Feb 2020 | 12:12:18 18 Feb 2020 | 21 seconds |
| Re-calibrating base error rates | 12:12:18 18 Feb 2020 | 12:12:21 18 Feb 2020 | 3 seconds |
| Examining read alignment evidence | 12:12:21 18 Feb 2020 | 12:22:09 18 Feb 2020 | 9 minutes 48 seconds |
| Polymorphism statistics | 12:22:09 18 Feb 2020 | 12:22:11 18 Feb 2020 | 2 seconds |
| Output | 12:22:11 18 Feb 2020 | 12:25:48 18 Feb 2020 | 3 minutes 37 seconds |
| Total | 26 minutes 59 seconds | ||
