New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	222422 =	53 (1.050)	3 (0.060)	3/246	NT	5.9%	coding (277/1020 nt)	PP_0170	ABC transporter substrate‑binding protein
	?	NC_002947	222477 =	46 (0.960)					coding (332/1020 nt)	PP_0170	ABC transporter substrate‑binding protein

AGGAGCAGGGCATCGACATCGAAGTCGATTGGGCGCAGCTGTCGGGTGGCTCGGCCGTCAACGATGCATTGTTGTCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCGCCGTTCCGGCGGTGGGTGTTTCGGTGCAGTCGCGCTTCCTGC  >  NC_002947/222330‑222623                                                                                                                                                    |                                                                                                                                                   gtcggagatgtcggcgatggtcttcacgttggggttgctgctgaccaggtagtacgggaagttgcccagcgaagccacggccttcacgttctGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCa                                                                                                                                                    >  7:164737‑M1/93‑148 (MQ=255)        gggCATCGACATCGAAGTCGATTGGGCGCAGCTGTCGGGTGGCTCGGCCGTCAACGATGCATTGTTGTCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGATCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGa                                                                                                                                            <  4:243700/149‑1 (MQ=255)        gggCATCGACATCGAAGTCGATTGGGCGCAGCTGTCGGGTGGCTCGGCCGTCAACGATGCATTGTTGTCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGa                                                                                                                                            <  2:37692/149‑1 (MQ=255)                        gTCGATTGGGCGCAGCTGTCGGGTGGCTCGGCCGTCAACGATGCATTGTTGTCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCt                                                                                                                            >  4:119630/1‑149 (MQ=255)                          cGATTGGGCGCAGCTGTCGGGTGGCTCGGCCGTCAACGATGCATTGTTGTCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTgg                                                                                                                          >  8:83355/1‑149 (MQ=255)                            aTTGGGCGCAGCTGTCGGGTGGCTCGGCCGTCAACGATGCATTGTTGTCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTggg                                                                                                                         >  4:196016/1‑148 (MQ=255)                            aTTGGGCGCAGCTGTCGGGTGGCTCGGCCGTCAACGATGCATTGTTGTCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGc                                                                                                                        <  7:83355/149‑1 (MQ=255)                                   gcAGCTGTCGGGTGGCTCGGCCGTCAACGATGCATTGTTGTCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTcc                                                                                                                 <  6:75438/149‑1 (MQ=255)                                   gcAGCTGTCGGGTGGCTCGGCCGTCAACGATGCATTGTTGTCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTcc                                                                                                                 >  2:284032/1‑149 (MQ=255)                                      gCTGTCGGGTGGCTCGGCCGTCAACGATGCTTTGTTGTCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGt                                                                                                              <  3:161394/149‑1 (MQ=255)                                        tGTCGGGTGGCTCGGCCGTCAACGATGCATTGTTGTCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGta                                                                                                             >  8:267202/1‑148 (MQ=255)                                          tCGGGTGGCTCGGCCGTCAACGATGCATTGTTGTCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGta                                                                                                             <  7:267202/146‑1 (MQ=255)                                                  cTCGGCCGTCAACGATGCATTGTTGTCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTg                                                                                                                                             <  1:166686/106‑1 (MQ=255)                                                  cTCGGCCGTCAACGATGCATTGTTGTCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTg                                                                                                                                             >  2:166686/1‑106 (MQ=255)                                                     ggCCGTCAACGATGCATTGTTGTCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAg                                                                                                                                          >  5:182103/1‑106 (MQ=255)                                                     ggCCGTCAACGATGCATTGTTGTCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAg                                                                                                                                          <  6:182103/106‑1 (MQ=255)                                                     ggCCGTCAACGATGCATTGTTGTCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTcagca                                                                                               <  1:4944/149‑1 (MQ=255)                                                     ggCCGTCAACGATGCATTGTTGTCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTcag                                                                                                 <  3:196016/147‑1 (MQ=255)                                                                 tGCATTGTTGTCCGGTGCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAAc                                                                                    <  8:217222/148‑1 (MQ=255)                                                                    aTTGTTGTCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTcagc                                                                                                >  2:151722/1‑133 (MQ=255)                                                                    aTTGTTGTCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTcagc                                                                                                <  1:151722/133‑1 (MQ=255)                                                                          gtCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAAc                                                                                    >  7:899/1‑139 (MQ=255)                                                                          gtCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAAc                                                                                    <  8:899/139‑1 (MQ=255)                                                                               gTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAg                                                                              >  6:215110/1‑140 (MQ=255)                                                                               gTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAg                                                                              <  5:215110/140‑1 (MQ=255)                                                                               gTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGcc                                                                     >  7:178551/1‑149 (MQ=255)                                                                                ttCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGcc                                                                     >  4:214554/1‑148 (MQ=255)                                                                                   ggTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGCCCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCACCCCCAACGTGAAGACCCTCGCCGACa                                                                 >  5:261927/1‑149 (MQ=255)                                                                                   ggTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGAc                                                                  <  1:284032/148‑1 (MQ=255)                                                                                          aTCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGcc                                                                     >  4:251452/1‑138 (MQ=255)                                                                                          aTCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGcc                                                                     <  3:251452/138‑1 (MQ=255)                                                                                            tgccggcccttggtgcggtcccagacggtcaacagcggtccgacaccggcgccggcAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGAc                                                         >  7:164737‑M2/57‑148 (MQ=255)                                                                                             gccggcccttggtgcggtcccagacggtcaacagcggtccgacaccggcgccggcAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAAc                                                                                    >  1:188196‑M2/56‑120 (MQ=255)                                                                                             gccggcccttggtgcggtcccagacggtcaacagcggtccgacaccggcgccggcAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAAc                                                                                    <  2:188196‑M2/65‑1 (MQ=255)                                                                                             GCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACa                                                        <  3:102995‑M1/148‑1 (MQ=255)                                                                                                    ccggTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCg                                                 >  6:215936/1‑148 (MQ=255)                                                                                                          tCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTccc                                                                                                                >  4:269505/1‑79 (MQ=255)                                                                                                          tCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTccc                                                                                                                <  3:269505/79‑1 (MQ=255)                                                                                                            ggACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCGCCGt                                        >  1:124583/1‑149 (MQ=255)                                                                                                               ccGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCGCCGTTcc                                     >  3:87109/1‑149 (MQ=255)                                                                                                                cGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCGCCGTTCcg                                    >  7:104040/1‑149 (MQ=255)                                                                                                                 gCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCGCCGTTCcgg                                   <  3:119630/149‑1 (MQ=255)                                                                                                                  cTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCGCCGTTCcggc                                  >  2:135434/1‑149 (MQ=255)                                                                                                                       gACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGaa                                                                               <  4:253942/99‑1 (MQ=255)                                                                                                                       gACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGaa                                                                               >  3:253942/1‑99 (MQ=255)                                                                                                                         ccGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCGCCGTTCCGGCggtgggt                           <  4:102995/149‑1 (MQ=255)                                                                                                                          cGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCGCCGTTCCGGCggtgggtg                          <  8:6985/149‑1 (MQ=255)                                                                                                                                ggACCGCACCAAGGGCCGGCAGACCGTGACCGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGGCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCGCCGTTCCGGCGGTGGGTGTTTCgg                    <  6:261927/149‑1 (MQ=255)                                                                                                                                 gACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCGCCGTTCCGGCGGTGGGTGTTTCGGt                   <  3:214554/149‑1 (MQ=255)                                                                                                                                 gACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCGCCGTTCCGGCGGTGGGTGTTTCGGt                   <  3:19895/149‑1 (MQ=255)                                                                                                                                 gACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCGCCGTTCCGGCGGTGGGTGTTTCGGt                   <  8:178551/149‑1 (MQ=255)                                                                                                                                         cAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCGCCGTTCCGGCGGTGGGTGTTTCGGTGCAGTcgc           >  7:124656/1‑149 (MQ=255)                                                                                                                                         cAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCGCCGTTCCGGCGGTGGGTGTTTCGGTGCAGTcgc           <  1:135434/149‑1 (MQ=255)                                                                                                                                          cggcgccggcAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCGCCGTTCCGGCGGTGGGTGTTTCGGTGCAGTcgcg          <  8:164737‑M2/139‑1 (MQ=255)                                                                                                                                            gggCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCg                                            <  3:250162/113‑1 (MQ=255)                                                                                                                                            gggCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCg                                            >  4:250162/1‑113 (MQ=255)                                                                                                                                            gggCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCGCCGTTCCGGCGGTGGGTGTTTCTGTGCAGTCGCGCt        <  8:104040/149‑1 (MQ=255)                                                                                                                                             ggCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCGCCGTTCCGGCGGTGGGTGTTTCGGt                   <  7:65727/137‑1 (MQ=255)                                                                                                                                             ggCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCGCCGTTCCGGCGGTGGGTGTTTCGGt                   >  8:65727/1‑137 (MQ=255)                                                                                                                                                  gcAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCGCCGTTCCGGCGGTGGGTGTTTCGGTGCAGTCGCGCTTCCTGc  <  5:215936‑M2/147‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   AGGAGCAGGGCATCGACATCGAAGTCGATTGGGCGCAGCTGTCGGGTGGCTCGGCCGTCAACGATGCATTGTTGTCCGGTTCGGTGGACATCGCCGGCGCCGGTGTCGGACCGCTGTTGACCGTCTGGGACCGCACCAAGGGCCGGCAGAACGTGAAGGCCGTGGCTTCGCTGGGCAACTTCCCGTACTACCTGGTCAGCAGCAACCCCAACGTGAAGACCATCGCCGACATCTCCGACAAAGACCGTATCGCCGTTCCGGCGGTGGGTGTTTCGGTGCAGTCGCGCTTCCTGC  >  NC_002947/222330‑222623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.