mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | glucose-4_S38_L001_R1_001.good.fq | 183,714 | 26,844,304 | 100.0% | 146.1 bases | 148 bases | 97.3% |
| errors | glucose-4_S38_L001_R2_001.good.fq | 183,714 | 26,827,947 | 100.0% | 146.0 bases | 148 bases | 96.5% |
| errors | glucose-4_S38_L002_R1_001.good.fq | 164,438 | 24,020,024 | 100.0% | 146.1 bases | 148 bases | 97.2% |
| errors | glucose-4_S38_L002_R2_001.good.fq | 164,438 | 24,008,476 | 100.0% | 146.0 bases | 148 bases | 96.0% |
| errors | glucose-4_S38_L003_R1_001.good.fq | 169,473 | 24,760,065 | 100.0% | 146.1 bases | 148 bases | 97.2% |
| errors | glucose-4_S38_L003_R2_001.good.fq | 169,473 | 24,744,930 | 100.0% | 146.0 bases | 148 bases | 96.2% |
| errors | glucose-4_S38_L004_R1_001.good.fq | 156,677 | 22,884,005 | 100.0% | 146.1 bases | 148 bases | 97.2% |
| errors | glucose-4_S38_L004_R2_001.good.fq | 156,677 | 22,873,050 | 100.0% | 146.0 bases | 148 bases | 95.7% |
| total | 1,348,604 | 196,962,801 | 100.0% | 146.0 bases | 148 bases | 96.7% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_002947 | 6,181,873 | 30.7 | 1.8 | 100.0% | Pseudomonas putida KT2440 chromosome, complete genome. |
| total | 6,181,873 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 17514 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 1020 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.050 |
| reference sequence | pr(no read start) |
|---|---|
| NC_002947 | 0.90827 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.3.4.1 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 07:37:40 18 Feb 2020 | 07:38:01 18 Feb 2020 | 21 seconds |
| Read alignment to reference genome | 07:38:01 18 Feb 2020 | 07:41:01 18 Feb 2020 | 3 minutes 0 seconds |
| Preprocessing alignments for candidate junction identification | 07:41:01 18 Feb 2020 | 07:41:20 18 Feb 2020 | 19 seconds |
| Preliminary analysis of coverage distribution | 07:41:20 18 Feb 2020 | 07:42:06 18 Feb 2020 | 46 seconds |
| Identifying junction candidates | 07:42:06 18 Feb 2020 | 07:42:14 18 Feb 2020 | 8 seconds |
| Re-alignment to junction candidates | 07:42:14 18 Feb 2020 | 07:43:18 18 Feb 2020 | 1 minute 4 seconds |
| Resolving best read alignments | 07:43:18 18 Feb 2020 | 07:43:48 18 Feb 2020 | 30 seconds |
| Creating BAM files | 07:43:48 18 Feb 2020 | 07:44:29 18 Feb 2020 | 41 seconds |
| Tabulating error counts | 07:44:29 18 Feb 2020 | 07:44:44 18 Feb 2020 | 15 seconds |
| Re-calibrating base error rates | 07:44:44 18 Feb 2020 | 07:44:46 18 Feb 2020 | 2 seconds |
| Examining read alignment evidence | 07:44:46 18 Feb 2020 | 07:47:54 18 Feb 2020 | 3 minutes 8 seconds |
| Polymorphism statistics | 07:47:54 18 Feb 2020 | 07:47:54 18 Feb 2020 | 0 seconds |
| Output | 07:47:54 18 Feb 2020 | 07:48:15 18 Feb 2020 | 21 seconds |
| Total | 10 minutes 35 seconds | ||
