breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 202,267 C→T P56L (CCT→CTT)  fabZ → (3R)‑hydroxymyristol acyl carrier protein dehydratase
MC JC 257,908 Δ776 bp [crl] [crl]
RA 668,742 C→G E99Q (GAA→CAA)  rsfS ← ribosomal silencing factor
JC JC 1,677,978 IS5 (+) +4 bp intergenic (‑75/‑446) pntA ← / → ydgH pyridine nucleotide transhydrogenase, alpha subunit/DUF1471 family periplasmic protein
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,513,351 C→A L104M (CTG→ATG)  nupC → nucleoside (except guanosine) transporter
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,960,918 T→G I81S (ATC→AGC)  rep → DNA helicase and single‑stranded DNA‑dependent ATPase
RA 4,159,842 T→C S317G (AGC→GGC)  sthA ← pyridine nucleotide transhydrogenase, soluble
RA 4,184,968 A→C K1242Q (AAG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,188,869 C→A R1174S (CGT→AGT)  rpoC → RNA polymerase, beta prime subunit
MC JC 4,233,804 1557 bp→83 bp coding (47‑1603/1650 nt) pgi → glucosephosphate isomerase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1299302 1300828 1527 45 [42] [43] 46 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* * ÷ NC_000913 1978347 1979667 1321 45 [43] [39] 45 insB1–insA insB1, insA
* * ÷ NC_000913 2469086 2470744 1659 46 [40] [44] 48 [gtrB]–gtrS [gtrB], gtrS
* * ÷ NC_000913 3423837–3424234 3424514–3424238 5–678 45 [40] [44] 49 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =6 (0.060)99 (0.980) 68/464 0.2 96.3% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 2 (0.020)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12078056 (0.060)99 (0.980) 70/464 0.1 96.3% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 2 (0.020)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related