breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 450,073 C→T W190* (TGG→TAG)  cyoB ← cytochrome o ubiquinol oxidase subunit I
RA 850,651 T→G Y68D (TAC→GAC)  ompX → outer membrane protein X
RA 1,818,103 A→T V133E (GTA→GAA)  chbF ← phospho‑chitobiase; general 6‑phospho‑beta‑glucosidase activity
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,907,364 A→T L193Q (CTG→CAG)  eno ← enolase
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1896893 1898325 1433 67 [64] [63] 67 sdaA sdaA
* * ÷ NC_000913 2684230 2685556 1327 65 [64] [64] 65 glyA glyA
* * ÷ NC_000913 2929531 2930994 1464 66 [64] [64] 65 sdaB sdaB
* * ÷ NC_000913 3258262 3259703 1442 65 [64] [64] 67 tdcG tdcG
* * ÷ NC_000913 3423741–3424234 3424563–3424238 5–823 65 [64] [64] 66 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 85009139 (0.300)109 (0.850) 89/296 0.4 67.1% coding (7/888 nt) rhtA threonine and homoserine efflux system
?NC_000913 852947 = 68 (0.530)coding (51/129 nt) mntS Mn(2)‑response protein, MntR‑repressed
* ? NC_000913 1207790 =15 (0.120)73 (0.630) 60/266 1.5 82.8% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 17 (0.150)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780515 (0.120)68 (0.590) 59/266 1.6 81.7% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 17 (0.150)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)120 (0.950) 92/290 0.3 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein