breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 757 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,850,0070GA50.5% 79.5 / 163.7 107P44S (CCG→TCG) uhpBsensory histidine kinase in two‑component regulatory sytem with UhpA
*NC_000913501,5750AC48.5% 225.9 / 23.7 132V555G (GTG→GGG) ybaLinner membrane putative NAD(P)‑binding transporter
*NC_00091329,4960TA46.3% 135.5 / 85.1 134intergenic (+301/‑155)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
*NC_0009132,603,3420AC45.3% 133.8 / 113.4 117T509P (ACC→CCC) hyfBhydrogenase 4, membrane subunit
*NC_00091329,4860CA44.9% 185.6 / 76.6 136intergenic (+291/‑165)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
*NC_0009132,434,8820TG44.6% 189.4 / 30.7 121Y252S (TAC→TCC) truAtRNA pseudouridine(38‑40) synthase
*NC_0009133,926,2100TG44.0% 111.8 / 315.4 200A82A (GCA→GCCmioCFMN‑binding protein MioC
*NC_000913298,9600AC43.9% 192.1 / 47.4 131Y659D (TAT→GAT) paoCPaoABC aldehyde oxidoreductase, Moco‑containing subunit
*NC_000913213,3410AG43.8% 125.1 / 32.4 90G337G (GGA→GGGtilStRNA(Ile)‑lysidine synthetase
*NC_000913357,8900CA43.8% 125.9 / 56.7 105L267F (TTG→TTTcynRtranscriptional activator of cyn operon; autorepressor
*NC_0009131,982,5230AG43.8% 222.6 / 43.6 137intergenic (‑136/+31)otsB/araHtrehalose‑6‑phosphate phosphatase, biosynthetic/L‑arabinose ABC transporter permease
*NC_0009134,538,1090AC43.7% 201.3 / 36.7 119G219G (GGT→GGGnanMN‑acetylneuraminic acid mutarotase
*NC_0009132,386,8620GA43.5% 135.0 / 112.1 138intergenic (+31/‑72)yfbL/yfbMputative M28A family peptidase/DUF1877 family protein
*NC_0009133,705,6610TC43.5% 215.4 / 42.6 138I44V (ATC→GTC) dppBdipeptide/heme ABC transporter permease
*NC_0009131,622,8160GA43.3% 110.3 / 132.3 134intergenic (+75/+144)yneM/dgcZinner membrane‑associated protein/diguanylate cyclase, zinc‑sensing
*NC_000913501,5640AC43.1% 267.2 / 18.8 138*559E (TAA→GAA) ybaLinner membrane putative NAD(P)‑binding transporter
*NC_0009131,004,6250TG42.4% 220.4 / 20.1 125L227V (TTG→GTG) ycbFputative periplasmic pilin chaperone
*NC_0009134,332,7910AC42.0% 217.6 / 24.0 112V161G (GTG→GGG) basSsensory histidine kinase in two‑component regulatory system with BasR
*NC_000913357,8800TC41.8% 153.3 / 30.8 103K271E (AAA→GAA) cynRtranscriptional activator of cyn operon; autorepressor
*NC_000913366,5260TG41.6% 230.9 / 21.4 114T329P (ACC→CCC) lacIlactose‑inducible lac operon transcriptional repressor

Marginal new junction evidence (lowest skew 10 of 50 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 925901 =129 (0.990)42 (0.330)
+C
29/296 7.2 27.1% noncoding (71/88 nt) serW tRNA‑Ser
?NC_000913 = 1097399 99 (0.760)intergenic (+570/+166) ycdU/serX putative inner membrane protein/tRNA‑Ser
* ? NC_000913 = 3950349117 (0.900)29 (0.230) 25/286 8.0 22.8% coding (28/99 nt) ilvL ilvG operon leader peptide
?NC_000913 3952022 = 84 (0.680)pseudogene (481/663 nt) ilvG pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted
* ? NC_000913 1411899 =116 (0.890)7 (0.070) 7/246 13.9 6.5% coding (50/936 nt) ttcA tRNA s(2)C32 thioltransferase, iron sulfur cluster protein
?NC_000913 = 1434958 105 (0.980)pseudogene (51/51 nt) ttcC pseudogene, prophage Rac integration site ttcA duplication;Phage or Prophage Related
* ? NC_000913 925901 =129 (0.990)21 (0.160)
+C
8/296 15.2 19.3% noncoding (71/88 nt) serW tRNA‑Ser
?NC_000913 = 1097218 48 (0.370)intergenic (+389/+347) ycdU/serX putative inner membrane protein/tRNA‑Ser
* ? NC_000913 381258 =NA (NA)20 (0.160) 8/296 15.2 NA pseudogene (3/417 nt) yaiX pseudogene, interrupted by IS2A; hexapeptide transferase superfamily; putative transferase
?NC_000913 = 382589 NA (NA)noncoding (1330/1331 nt) IS2 repeat region
* ? NC_000913 4498181 =NA (NA)19 (0.150) 8/298 15.3 NA noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 4499512 NA (NA)intergenic (+12/+81) insD1/yjgX IS2 transposase TnpB/pseudogene fragment
* ? NC_000913 2882234 =168 (1.300)5 (0.040) 5/262 15.9 3.3% coding (404/483 nt) casB CRISP RNA (crRNA) containing Cascade antiviral complex protein
?NC_000913 2882252 = 145 (1.270)coding (386/483 nt) casB CRISP RNA (crRNA) containing Cascade antiviral complex protein
* ? NC_000913 = 2518231137 (1.060)4 (0.040)
+23 bp
4/252 16.3 3.3% noncoding (1/76 nt) alaW tRNA‑Ala
?NC_000913 = 3427077 NA (NA)noncoding (76/77 nt) ileU tRNA‑Ile
* ? NC_000913 225456 =12 (0.090)4 (0.040)
+23 bp
4/252 16.3 6.0% noncoding (76/77 nt) ileV tRNA‑Ile
?NC_000913 = 2518231 137 (1.060)noncoding (1/76 nt) alaW tRNA‑Ala
* ? NC_000913 = 2518117145 (1.120)4 (0.040)
+22 bp
4/254 16.4 3.1% intergenic (‑1/+39) alaX/alaW tRNA‑Ala/tRNA‑Ala
?NC_000913 = 3427077 NA (NA)noncoding (76/77 nt) ileU tRNA‑Ile