breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE512,6430AG53.3% 20.5 / 104.1 75intergenic (+711/‑6052)recR/speA_2Recombination protein RecR/Arginine decarboxylase
*USA300TCH1516_ALE2,845,6890GA31.6% 23.0 / 11.6 19A77A (GCC→GCTUSA300HOU_RS14575hypothetical protein
*USA300TCH1516_ALE2,845,7070AG30.0% 22.6 / 12.1 20F71F (TTT→TTCUSA300HOU_RS14575hypothetical protein
*USA300TCH1516_ALE872,4730TC28.6% 48.6 / 24.0 35N57N (AAT→AACUSA300HOU_RS04265hypothetical protein
*USA300TCH1516_ALE2,845,7220AG28.6% 24.7 / 13.8 21H66H (CAT→CACUSA300HOU_RS14575hypothetical protein
*USA300TCH1516_ALE872,5200AG27.3% 30.8 / 10.8 22E73G (GAG→GGG) USA300HOU_RS04265hypothetical protein
*USA300TCH1516_ALE247,3810CA26.1% 46.5 / 11.0 23I72I (ATC→ATAcycBCyclodextrin‑binding protein
*USA300TCH1516_ALE2,845,6680GA26.1% 35.8 / 10.9 23C84C (TGC→TGTUSA300HOU_RS14575hypothetical protein
*USA300TCH1516_ALE1,646,1290GA25.7% 65.6 / 15.3 35A175V (GCA→GTA) ypdFAminopeptidase YpdF
*USA300TCH1516_ALE2,845,6320TG25.0% 31.6 / 16.4 24G96G (GGA→GGCUSA300HOU_RS14575hypothetical protein
*USA300TCH1516_ALE2,845,6650CT25.0% 40.0 / 12.1 24S85S (TCG→TCAUSA300HOU_RS14575hypothetical protein
*USA300TCH1516_ALE795,6420AG22.9% 59.7 / 18.6 35I188V (ATC→GTC) recQ_1ATP‑dependent DNA helicase RecQ
*USA300TCH1516_ALE115,8230CT21.6% 74.2 / 15.9 37intergenic (+90/‑61)btr/yxeP_1HTH‑type transcriptional activator Btr/putative hydrolase YxeP
*USA300TCH1516_ALE111,5160CA20.6% 179.0 / 29.3 68Q196K (CAA→AAA) USA300HOU_RS00520putative lipoprotein
*USA300TCH1516_ALE2,263,6570TC20.4% 98.1 / 23.9 49N286N (AAT→AACczcD_2Cadmium, cobalt and zinc/H(+)‑K(+) antiporter
*USA300TCH1516_ALE111,5120AG20.3% 139.0 / 35.3 69S194S (TCA→TCGUSA300HOU_RS00520putative lipoprotein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000731 = 1610101 (0.840)4 (0.010)
+22 bp
3/250 10.5 0.83% intergenic (+395/‑461) repA/USA300HOU_pUSA300HOUMR0003 replication protein A/hypothetical protein
?NC_012417 = 1057 1030 (0.950)coding (138/192 nt) USA300HOU_RS15665 hypothetical protein
* ? CP000731 = 4399130 (1.080)4 (0.010) 3/272 11.1 0.66% intergenic (+364/‑101) USA300HOU_pUSA300HOUMR0006/cadR hypothetical protein/cadmium binding protein
?NC_012417 = 1963 1077 (1.070)intergenic (+196/+130) USA300HOU_RS14895/USA300HOU_RS14900 hypothetical protein/hypothetical protein
* ? CP000731 = 2352165 (1.370)4 (0.010) 3/272 11.1 0.65% intergenic (+132/+36) USA300HOU_pUSA300HOUMR0003/USA300HOU_pUSA300HOUMR0004 hypothetical protein/hypothetical protein
?NC_012417 = 1963 1077 (1.070)intergenic (+196/+130) USA300HOU_RS14895/USA300HOU_RS14900 hypothetical protein/hypothetical protein
* ? NC_012417 = 10571030 (0.950)4 (0.000) 4/250 52.6 0.45% coding (138/192 nt) USA300HOU_RS15665 hypothetical protein
?NC_012417 = 1103 879 (0.960)coding (92/192 nt) USA300HOU_RS15665 hypothetical protein
* ? NC_012417 = 19741162 (1.070)5 (0.000) 4/272 54.7 0.48% intergenic (+207/+119) USA300HOU_RS14895/USA300HOU_RS14900 hypothetical protein/hypothetical protein
?NC_012417 = 2041 989 (0.990)intergenic (+274/+52) USA300HOU_RS14895/USA300HOU_RS14900 hypothetical protein/hypothetical protein