New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	= 613929	21 (0.690)	5 (0.180)	3/262	3.6	18.3%	coding (2241/2844 nt)	sdrC	Serine‑aspartate repeat‑containing protein C
	?	USA300TCH1516_ALE	614110 =	25 (0.880)					coding (2422/2844 nt)	sdrC	Serine‑aspartate repeat‑containing protein C
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGAT‑TCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTC  >  USA300TCH1516_ALE/613972‑614249                                                                                                                                            |                                                                                                                                            tcagactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGAT‑TCAGATTCAGACTCa                                                                                                                                           >  1:299393/1‑141 (MQ=255)        cagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGAT‑TCAGATTCAGACTCAGATAGCg                                                                                                                                    >  1:147026/1‑141 (MQ=255)                                gCGACACAGACTCAGATGGTGACTCAGACTCTGGTGGCGACTCAGACGTAGATAGTGATTCAGATGAAGATAGCGATTCGGACTCAGACAGTGAT‑TCAGATTCAGCCTCAGATAGCGACTCAGATTCTGACAGCGATtcag                                                                                                            <  2:270926/141‑1 (MQ=21)                                    ctcagactcagaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGAT‑TCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATtcagactc                                                                                                        >  1:163021/1‑141 (MQ=255)                                     tcagactcagaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGAT‑TCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATtcagactca                                                                                                       <  1:292206/141‑1 (MQ=255)                                             agaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGAT‑TCAGATTCAGACTGAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCCGTCAGCga                                                                                               >  1:94947/1‑141 (MQ=255)                                              gagcgCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGAG‑TCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCgac                                                                                              <  1:343473/137‑1 (MQ=255)                                                tgacGACGCAGATGCGGATAGCGACTCAGACGCAGAGAGCGATGCAGATGCAGATAGCGATTCGGACTCAGACAGTGAT‑TCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCgactc                                                                                            <  2:24003/138‑1 (MQ=25)                                                 aggaagacAGATTAGGATAGCGAAGAAGAAGAAGAGAGCAAGTCAGATGAAGATATCGAATCTGACTAAGACAGCGAC‑TAAGACTAAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCgactca                                                                                           <  2:322421/134‑1 (MQ=12)                                                  gCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGAT‑TCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCgactcag                                                                                          >  1:228259/1‑141 (MQ=255)                                                  gCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGAT‑TCAGATTCAGACTCAGATAGAGATTCAGATGCAGACAGCGATTCAGACACAGATAACgactcaa                                                                                          >  2:154396/1‑140 (MQ=21)                                                              cGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGAT‑TCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTg                                                                              >  1:277336/1‑141 (MQ=255)                                                              cGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGAT‑TCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGAATCAGACCGTg                                                                              >  1:190016/1‑141 (MQ=255)                                                               ggATAGCGACTCAGACTCAGATAGCGATTCAGATGCAGATAGCGATTCGGACTCAGACAGTGAT‑TCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTga                                                                             <  1:191198/141‑1 (MQ=255)                                                                  tggCGACTCAGACTCAGATAGCGACTCAGATTCAGAGTGCGATTTGTACACAGTCAGGCAT‑CCAGATAGAGACTCAGCTAGCGACTCAGTCTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTgattc                                                                          <  2:147026/139‑1 (MQ=18)                                                                              ctcagaTAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGAT‑TCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAg                                                              <  1:325498/141‑1 (MQ=255)                                                                               tcagaTAGCGATTCAGATTCAGATAGCGATTCGGCAAAAGA‑AGGGATGGCTGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGc                                                             <  1:66161/141‑1 (MQ=17)                                                                                        gactcagactcagatAGCGATTCGGACTCAGACAGTGAT‑TCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagat                                                    >  1:351960/4‑141 (MQ=255)                                                                                        gactcagactcagatAGCGATTCGGACTCAGACAGTGAT‑TCAGATTCAGACACAGATAGCGACTCAGATTCTGACAGAGATTCAGACTCAGACAGCGACTCAGACACAGACAGAGATTCAGATTCAGAAAGCGACacacat                                                    >  2:111083/4‑141 (MQ=18)                                                                                          ttcagattcagaTAGCGATTCGGACTCAGACAGTGAT‑TCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagattc                                                  <  1:21104/141‑1 (MQ=255)                                                                                              gattcagacAGCGATTCGGACTCAGACAGTGAT‑TCAGATGCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagattcagat                                              <  1:52813/141‑1 (MQ=255)                                                                                                    tacaccGATTCGGACTCAGACACTGAT‑TCTGATTCAGTCTCAGATCGCGACTCAGAGACTGATAGCGATTCAGTCTCAGACAGCGCCTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgac                                        <  2:228259/136‑1 (MQ=17)                                                                                                                   tCAGACAGTGAT‑TCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACCGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagc                         >  1:83056/1‑141 (MQ=255)                                                                                                                      gACAGTGAT‑TCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGCCTCAGAGTCAGACAGTGATTCAGATTCAGACAGCGACACAGATacagctagcgactcagactcagatacagac                      >  2:215299/1‑136 (MQ=18)                                                                                                                                 cagattcagaCTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAACAACACAAAATCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcagactcag            >  1:8779/1‑141 (MQ=17)                                                                                                                                           cTCAGATAGCGACTCAGATTCTGCCAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcaaatagcgactcagactcagatagcgactccgactccgatcgcgccta  >  2:236699/1‑140 (MQ=14)                                                                                                                                           cTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGGCTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactcagactcagatagcgactc  >  2:345667/1‑141 (MQ=255)                                                                                                                                           cTCAGATACCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCCGATtcagatagcgactcagactcagatagcgactcagactcagatagcgactc  <  2:24094/141‑1 (MQ=14)                                                                                                                                            |                                                                                                                                            TCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGAT‑TCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTC  >  USA300TCH1516_ALE/613972‑614249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.