breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
read file | reads | bases | passed filters | average | longest | mapped | |
---|---|---|---|---|---|---|---|
errors | SNFM_15_54_1_S209_S1992_L006_R1_001.good.fq | 1,015,680 | 137,104,696 | 100.0% | 135.0 bases | 141 bases | 90.8% |
errors | SNFM_15_54_1_S209_S1992_L006_R2_001.good.fq | 1,015,680 | 137,104,696 | 100.0% | 135.0 bases | 141 bases | 89.5% |
errors | SNFM_15_54_1_S209_S1992_L008_R1_001.good.fq | 440,968 | 59,278,116 | 100.0% | 134.4 bases | 141 bases | 90.7% |
errors | SNFM_15_54_1_S209_S1992_L008_R2_001.good.fq | 440,968 | 59,278,116 | 100.0% | 134.4 bases | 141 bases | 88.5% |
total | 2,913,296 | 392,765,624 | 100.0% | 134.8 bases | 141 bases | 89.9% |
seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
---|---|---|---|---|---|---|---|
coverage | distribution | USA300TCH1516_ALE | 2,872,915 | 114.1 | 2.2 | 93.6% | Staphylococcus species strain strain. |
coverage | distribution | CP000731 | 27,041 | 385.6 | 3.9 | 2.9% | Staphylococcus aureus subsp. aureus USA300_TCH1516 plasmid |
coverage | distribution | NC_012417 | 3,125 | 4068.3 | 13.7 | 3.4% | Staphylococcus aureus subsp. aureus USA300_TCH1516 plasmid |
total | 2,903,081 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
option | limit | actual |
---|---|---|
Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 100000 |
Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 404 |
Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.040 |
reference sequence | pr(no read start) |
---|---|
USA300TCH1516_ALE | 0.69570 |
CP000731 | 0.37257 |
NC_012417 | 0.04021 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
option | value |
---|---|
Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
option | value |
---|---|
Mode | Consensus/Mixed Base |
Ploidy | 1 (haploid) |
Consensus mutation E-value cutoff | 10 |
Consensus frequency cutoff | 0.75 |
Consensus minimum variant coverage each strand | OFF |
Consensus minimum total coverage each strand | OFF |
Consensus minimum variant coverage | OFF |
Consensus minimum total coverage | OFF |
Polymorphism E-value cutoff | 10 |
Polymorphism frequency cutoff | 0.2 |
Polymorphism minimum variant coverage each strand | OFF |
Polymorphism minimum total coverage each strand | OFF |
Polymorphism minimum variant coverage | OFF |
Polymorphism minimum total coverage | OFF |
Polymorphism bias cutoff | OFF |
Predict indel polymorphisms | YES |
Skip indel polymorphisms in homopolymers runs of | OFF |
Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
program | version |
---|---|
bowtie2 | 2.3.4.1 |
R | 3.4.4 |
step | start | end | elapsed |
---|---|---|---|
Read and reference sequence file input | 07:24:37 24 May 2019 | 07:25:20 24 May 2019 | 43 seconds |
Read alignment to reference genome | 07:25:20 24 May 2019 | 07:32:20 24 May 2019 | 7 minutes 0 seconds |
Preprocessing alignments for candidate junction identification | 07:32:20 24 May 2019 | 07:33:03 24 May 2019 | 43 seconds |
Preliminary analysis of coverage distribution | 07:33:03 24 May 2019 | 07:34:21 24 May 2019 | 1 minute 18 seconds |
Identifying junction candidates | 07:34:21 24 May 2019 | 07:37:01 24 May 2019 | 2 minutes 40 seconds |
Re-alignment to junction candidates | 07:37:01 24 May 2019 | 07:39:15 24 May 2019 | 2 minutes 14 seconds |
Resolving best read alignments | 07:39:15 24 May 2019 | 07:40:18 24 May 2019 | 1 minute 3 seconds |
Creating BAM files | 07:40:18 24 May 2019 | 07:41:24 24 May 2019 | 1 minute 6 seconds |
Tabulating error counts | 07:41:24 24 May 2019 | 07:41:54 24 May 2019 | 30 seconds |
Re-calibrating base error rates | 07:41:54 24 May 2019 | 07:41:56 24 May 2019 | 2 seconds |
Examining read alignment evidence | 07:41:56 24 May 2019 | 07:47:26 24 May 2019 | 5 minutes 30 seconds |
Polymorphism statistics | 07:47:26 24 May 2019 | 07:47:28 24 May 2019 | 2 seconds |
Output | 07:47:28 24 May 2019 | 07:47:45 24 May 2019 | 17 seconds |
Total | 23 minutes 8 seconds |