breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation annotation gene description
RA CP000731 81 N→G intergenic (–/‑151)  / → repA –/replication protein A
JC CP000731 6,552 +28 bp intergenic (+1069/‑419) cadX → / → USA300HOU_pUSA300HOUMR0010 cadmium efflux regulator/MarR family transcriptional regulator
RA CP000731 25,669 (A)7→6 coding (322/333 nt) USA300HOU_pUSA300HOUMR0031 → hypothetical protein
JC USA300TCH1516_ALE 62,818 +CAATTT intergenic (‑285/+228) USA300TCH1516_00053 ← / ← speG hypothetical protein/Spermidine N(1)‑acetyltransferase
RA USA300TCH1516_ALE 742,735 C→T intergenic (+572/‑125) ybhL → / → melR_1 Inner membrane protein YbhL/Melibiose operon regulatory protein
RA USA300TCH1516_ALE 849,203 A→G Y110C (TAT→TGT)  USA300HOU_RS04145 → Nucleotide‑binding protein
RA USA300TCH1516_ALE 1,416,458 A→T I216N (ATT→AAT)  oppD_4 ← Oligopeptide transport ATP‑binding protein OppD
RA USA300TCH1516_ALE 1,513,930 A→T L413F (TTA→TTT USA300HOU_RS07375 → hypothetical protein
RA USA300TCH1516_ALE 1,646,189 G→C A155G (GCA→GGA)  ypdF ← Aminopeptidase YpdF
RA USA300TCH1516_ALE 1,774,221 G→T A27D (GCC→GAC)  hemA ← Glutamyl‑tRNA reductase
RA USA300TCH1516_ALE 1,891,904 (T)6→5 coding (4/402 nt) rot ← HTH‑type transcriptional regulator rot
RA USA300TCH1516_ALE 1,902,155 C→T A105A (GCC→GCT arsB → Arsenical pump membrane protein
RA USA300TCH1516_ALE 2,010,363 C→T C146Y (TGT→TAT)  USA300HOU_RS10125 ← Putative multidrug export ATP‑binding/permease protein
RA USA300TCH1516_ALE 2,279,705 G→A A943V (GCA→GTA)  ebh_3 ← Extracellular matrix‑binding protein ebh
RA USA300TCH1516_ALE 2,373,019 G→A Q253* (CAG→TAG)  pbuG ← Guanine/hypoxanthine permease PbuG
RA USA300TCH1516_ALE 2,532,298 A→T T15T (ACA→ACT femA_3 → Aminoacyltransferase FemA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ USA300TCH1516_ALE 2176703–2181102 2181102 1–4400 9 [6] [7] 8 ilvA/USA300TCH1516_02069 L‑threonine dehydratase biosynthetic IlvA/tRNA‑Gly

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE 512620 =34 (0.950)24 (0.940)
+40 bp
16/200 0.3 49.8% intergenic (+688/‑6075) recR/speA_2 Recombination protein RecR/Arginine decarboxylase
?USA300TCH1516_ALE 1998278 = NA (NA)intergenic (‑6/+3329) USA300TCH1516_01882/USA300TCH1516_01883 tRNA‑Val/tRNA‑Ala
* ? USA300TCH1516_ALE 2085296 =38 (1.060)15 (0.470) 8/252 1.6 35.2% coding (173/201 nt) hlb_1 Phospholipase C
?USA300TCH1516_ALE = 2128343 21 (0.650)coding (1/825 nt) hlb_2 Phospholipase C
* ? USA300TCH1516_ALE 2476329 =30 (0.840)9 (0.260) 5/268 2.6 23.5% intergenic (‑74/+166) tcaA/tcaR Membrane‑associated protein TcaA/HTH‑type transcriptional regulator TcaR
?USA300TCH1516_ALE = 2476325 30 (0.880)intergenic (‑70/+170) tcaA/tcaR Membrane‑associated protein TcaA/HTH‑type transcriptional regulator TcaR