Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	CP000731	6,552	+TCTCCAGGCACTTGTGCATATTCTGAAA	intergenic (+1069/‑419)	cadX → / → USA300HOU_pUSA300HOUMR0010	cadmium efflux regulator/MarR family transcriptional regulator

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000731	= 6552	0 (0.000)	37 (0.840) +TCTCCAGGCACTTGTGCATATTCTGAAA	30/200	0.5	100%	intergenic (+1069/‑419)	cadX/USA300HOU_pUSA300HOUMR0010	cadmium efflux regulator/MarR family transcriptional regulator
	?	CP000731	6553 =	0 (0.000)					intergenic (+1070/‑418)	cadX/USA300HOU_pUSA300HOUMR0010	cadmium efflux regulator/MarR family transcriptional regulator

GGTTGTGATTTGATATTCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP000731/6427‑6552 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACCAGGAATCATAGGATAATCT  >  CP000731/6553‑6680                                                                                                                               ||||||||||||||||||||||||||||                                                                                                                                 GGTTGGGATTTGATATTCATTGCATATTTTTCGAGTGCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCT                                                                                                                                                           <  2:222220/129‑1      TGATTTGATATTCATTACATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCTATATACCATAGACTCTTTAATAGACATTTCTTAGGGAATAGAAACAATACACTCTCCAaa                                                                                                                                                      >  2:85606/1‑127          TTGATATTCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGACTATGCCATATACCATAAACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACT                                                                                                                                                  >  1:157208/1‑129            GATATTCATTCCATATTTTGCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGTGGAATAGGAACAATCCACTCTCCAGGCACTTG                                                                                                                                                <  2:95342/129‑1                       CATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTG                                                                                                                                     >  2:150448/1‑129                       CATATTTTTCTAGATCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTCATAAACCTTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTG                                                                                                                                     >  2:39619/1‑129                           TTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACAATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAA                                                                                                                                 >  1:61644/1‑129                                 TAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACC                                                                                                                           >  1:152582/1‑129                                  AGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAAAAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCT                                                                                                                          >  2:213706/1‑129                                     TCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATACACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTA                                                                                                                       >  2:109676/1‑129                                             TGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATTCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCAC                                                                                                               <  1:197972/129‑1                                               ATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGC                                                                                                             <  2:182498/129‑1                                               ATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTCTGCATATTCTGAAAATCCACCTGTATGTGTCACGC                                                                                                             <  2:5363/129‑1                                                  ATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCAAGACACTTGTGCATATTATGAAAATCCACCTGTATGTGTAACGCCAA                                                                                                          >  2:132096/1‑129                                                       GCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATATGAACAATTCTCTCTCTTTGTATTTGTGTATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCA                                                                                                     <  1:199685/129‑1                                                                     TGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAA                                                                                       <  1:135139/129‑1                                                                      GTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTTACGCCAACATCATAACCAGTAACTAAT                                                                                      <  2:87877/129‑1                                                                       TGCCATATATCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATA                                                                                     <  2:144965/129‑1                                                                        GCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGTATAGGAACAATCCACTCTACAGAAACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCAAGCCAACATCATAACCAGTAACTAATAC                                                                                    >  1:101380/1‑129                                                                            TATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCTGGCTCTTGTGTATATTTTGATATTTCTCTTTTATTTGTCACGCCAACATCATAACCAGTAACTAATACATTA                                                                                <  1:64384/129‑1                                                                             ATACCATAGACTCTTTAATAGACATTTCTTGGGGACTGCTAACAATCTTCTCTCCAGGCACTTTTGCATATTCTGAAAATCCATCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTAT                                                                               <  1:222339/129‑1                                                                             ATACCATAGACTCTGTAATAGACATTTCTTGTGGAATAGGAACAATGCCCGCTCCAGGCACTCGTTCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTAT                                                                               <  2:229310/129‑1                                                                                   TAGTCTCTTTAATTGCAATATCTTGGTTAATAGGAACAATCCTCTCTCCAGGCACTTTTTCTTATTTTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTT                                                                         <  1:221054/129‑1                                                                                      gCTCTGTAGTAGACGTTTGTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTT                                                                      <  1:56823/128‑1                                                                                         CTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGGAAATCTACGTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAA                                                                   >  2:105290/1‑129                                                                                         CTTTAATAGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTAAAAATCCACATGTATGTGTCACGATAACATAGTAACCAGAAACTAATACATTATCGCCTTCTTTAA                                                                   >  1:42849/1‑129                                                                                                AGACATTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTACATATTCTAAAAATACACCTATATATGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATC                                                            >  1:90701/1‑129                                                                                                 GACAGTTCTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCA                                                           <  2:46495/129‑1                                                                                                   tatgTCTTGGCGAATAGGAACAAGCCACTCTCCAGGAACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCTAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATT                                                         <  2:77170/125‑1                                                                                                        CTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAAC                                                    >  1:156229/1‑129                                                                                                        CTTGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCAACCTGTATGTGTCACGACAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAAC                                                    >  1:134710/1‑129                                                                                                          tgtgtAATAGGAATAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTT                                                  <  1:82226/124‑1                                                                                                          TGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTT                                                  >  2:11319/1‑129                                                                                                          TGGGGAATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTT                                                  >  2:131552/1‑129                                                                                                           GGGGTATAGGAACAATCCACTCTCAAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCATGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTG                                                 <  1:85606/129‑1                                                                                                               AATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTAACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTC                                             >  1:169115/1‑129                                                                                                               AATAGGAACAATCCAATCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAATAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTacttt                                             >  2:11845/1‑124                                                                                                                ATAGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCA                                            >  2:135445/1‑129                                                                                                                  AGGAACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAAC                                          >  1:129092/1‑129                                                                                                                     AACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAAT                                       <  2:235159/129‑1                                                                                                                     AACAATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAAT                                       <  1:191824/129‑1                                                                                                                      ACAATCCCCTCTCCAGGCATTTGTGCATTTTCTGAAAAGCCACCTGTATGTGTCACGCCAACATCATATCCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATA                                      <  2:1405/129‑1                                                                                                                         ATCCACTCTCCAGGCACTTGTGCATATTCTGAAAATCAACTTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAAATTGATTCAACAATACCT                                   >  2:174255/1‑129                                                                                                                                   CAGGCACTTGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATTTCCGATCTGGAAAACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTA                         <  1:109676/129‑1                                                                                                                                           TGTGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACCAGGA                 <  2:17294/129‑1                                                                                                                                             TGCATATTCTGAAAATCCCCCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACCAGGAAT               <  1:150448/129‑1                                                                                                                                             TGCATATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAATAACTAATACATTAAAACCTTATTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACTAGGAAT               >  1:213915/1‑129                                                                                                                                                  ATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACCAGGAATCATAG          >  1:196672/1‑129                                                                                                                                                  ATTCTGAAAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACCAGGAATCATAG          >  1:113087/1‑129                                                                                                                                                   TTCTGATAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACCAGGAATCATAGG         <  2:171052/129‑1                                                                                                                                                         AAATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACCAGGAATCATAGGATAATC   <  2:129092/129‑1                                                                                                                                                          AATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACCAGGAATCATAGGATAATCT  <  1:213706/129‑1                                                                                                                                                          AATCCACCTGTATGTGTCACGCCAACATCATAACCACTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAACTCTATACCAGCAACCATAGCATCATCT  >  2:67224/1‑129                                                                                                                               ||||||||||||||||||||||||||||                                                                                                                                 GGTTGTGATTTGATATTCATTCCATATTTTTCTAGTTCAAAAATTGATAATGCTGCCGTAAAACCGGCTGTGCCATATACCATAGACTCTTTAATAGACATTTCTTGGGGAATAGGAACAATCCAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP000731/6427‑6552 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATCCACCTGTATGTGTCACGCCAACATCATAACCAGTAACTAATACATTATCGCCTTCTTTAAACTTATCATTCGAACTTGATTCAACAATACCTGCAAAATCTATACCAGGAATCATAGGATAATCT  >  CP000731/6553‑6680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.