New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	= 2748533	180 (0.880)	4 (0.020)	4/136	NT	2.3%	coding (140/444 nt)	hmoB	Heme‑degrading monooxygenase HmoB
	?	USA300TCH1516_ALE	= 2748555	190 (1.100)					coding (118/444 nt)	hmoB	Heme‑degrading monooxygenase HmoB
Rejected: Frequency below/above cutoff threshold.

TTAATGCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAAATGATTTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  USA300TCH1516_ALE/2748432‑2748533 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ataaatgatttaTCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACGTAATAAG  <  USA300TCH1516_ALE/2748555‑2748466                                                                                                                                                                                                  TTAATGCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATA                                                                                                       >  1:140541/1‑91 TTAATGCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATA                                                                                                       <  2:2888736/91‑1 TTAATGCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATA                                                                                                       <  2:3874957/91‑1 TTAATGCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATA                                                                                                       >  2:3290103/1‑91 TTAATGCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAAGGTTTCTTCTTGTCCTTCGTTAATATA                                                                                                       >  1:2999049/1‑91  TAATGCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATAT                                                                                                      >  2:579288/1‑91  TAATGCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATAT                                                                                                      <  2:829995/91‑1  TAATGCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATAT                                                                                                      >  2:332849/1‑91   AATGCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATA                                                                                                     >  1:697870/1‑91   AATGCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATA                                                                                                     >  1:1094733/1‑91   AATGCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATA                                                                                                     >  2:590132/1‑91   AATGCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGACGAAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATA                                                                                                     <  2:2063518/91‑1    tTGCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAA                                                                                                    <  2:3143451/90‑1    ATGCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCGAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAA                                                                                                    <  1:3140060/91‑1    ATGCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAA                                                                                                    >  2:3017074/1‑91    ATGCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAA                                                                                                    >  1:3487553/1‑91     tgtTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAAA                                                                                                   <  1:1545385/88‑1     TGCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAAA                                                                                                   >  1:2751094/1‑91     TGCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAAA                                                                                                   <  2:2049910/91‑1      GCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAAAT                                                                                                  <  2:3532713/91‑1      GCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAAAT                                                                                                  <  1:990720/91‑1       CTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTCTCTTCTTGTCCTTCGTTAATATATAAATG                                                                                                 >  1:2188959/1‑91       CTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTCTCTTCTTGTCCTTCGTTAATATATAAATG                                                                                                 >  1:2186759/1‑91       CTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTATCTTCTTGTCCTTCGTTAATATATAAATG                                                                                                 >  1:2186445/1‑91        TTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAAATGA                                                                                                >  1:1722121/1‑91         TTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAAATGAT                                                                                               >  2:2786481/1‑91         TTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAAATGAT                                                                                               >  1:326889/1‑91         TTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAAATGAT                                                                                               >  1:2782199/1‑91         TTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAAATGAT                                                                                               >  1:2781350/1‑91           AAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAACATTTTTGCTCGAATGTTTCTTCTTGTACTTCGTTAATATATAAATCATTT                                                                                             >  2:3668388/1‑91           AAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAAATGATTT                                                                                             <  2:2019419/91‑1            AATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAAATGATTTA                                                                                            >  1:648643/1‑91            AATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAAATGATTTA                                                                                            >  1:3088086/1‑91                                     TATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAAATGATTTATCAAATGATTCATTATGTGTGTTAA                                                                   <  1:2663468/91‑1                                                AAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAAATGATTTATCAAATGATTCATTATGTGTGTTAAATCATTTATAT                                                        <  2:1171608/91‑1                                                 AAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAAATGATTTATCAAATGATTCATTATGTGTGTTAAATCATTTATATA                                                       <  1:2517661/91‑1                                                  AAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAAATGATTTATCAAGTGATTCATTATGTGTGTTAAATCATTTATATAT                                                      <  1:3925719/91‑1                                                                                           ATAAATGATTTATGAAATGATTCATTATGTGTGTTAAATCATTGATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGC             <  1:3956349/91‑1                                                                                           ATAAATGATTTATCAAATGATTCATTATGTGTGTTAAATCATTGATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGC             <  1:3956335/91‑1                                                                                            TAAATGATCTATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACATGAAGAAACATTCGAGCAAAAATTTTTGCG            <  2:3437816/91‑1                                                                                             AAATGATTTATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGA           >  1:2468251/1‑91                                                                                              AATGATTTATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGAC          <  2:1160648/91‑1                                                                                              AATGATTTATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGAC          <  2:2361844/91‑1                                                                                              AATGATTTATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGAC          <  1:1809374/91‑1                                                                                               ATGATTTATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACG         >  2:3297854/1‑91                                                                                               ATGATTTATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACG         <  2:2564897/91‑1                                                                                               ATGATTTATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACG         >  2:3297790/1‑91                                                                                               ATGATTTAACAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGAAAAAATTTTTGCGACG         <  2:2566628/91‑1                                                                                                 GATTTATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACGTA       <  2:859887/91‑1                                                                                                  ATTTATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACGTAA      <  1:2189378/91‑1                                                                                                  ATTTATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACGTAA      >  2:34826/1‑91                                                                                                   TTTATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACGTAAT     <  2:3818277/91‑1                                                                                                   TTTATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACGTAAT     >  1:1939003/1‑91                                                                                                   TTTATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACGTAAT     >  2:536049/1‑91                                                                                                   TTTATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACGTAAT     <  1:3074344/91‑1                                                                                                   TTTATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACGTAAT     >  2:756830/1‑91                                                                                                    TTATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACGTAATA    <  2:365852/91‑1                                                                                                    TTATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACGTAATA    >  1:3570307/1‑91                                                                                                     TATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACGTAATAA   >  2:1625543/1‑91                                                                                                     TATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACGTAATAA   >  2:1575947/1‑91                                                                                                     TATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACGTAATAA   >  1:405239/1‑91                                                                                                     TATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACGTAATAA   <  1:599253/91‑1                                                                                                      ATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACGTAATAAG  <  1:845219/91‑1                                                                                                      ATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACGTAATAAG  >  2:555219/1‑91                                                                                                      ATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACGTAATAAG  <  1:2910265/91‑1                                                                                                      ATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACGTAATAAG  <  1:2910235/91‑1                                                                                                      ATCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACGTAATAAG  <  1:1344001/91‑1                                                                                                                                                                                                  TTAATGCTTTAAATCCATCAACGTTTTTTAAATGCTTATTACGTCGCAAAAATTTTTGCTCGAATGTTTCTTCTTGTCCTTCGTTAATATATAAATGATTTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  USA300TCH1516_ALE/2748432‑2748533 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ataaatgatttaTCAAATGATTCATTATGTGTGTTAAATCATTTATATATTAACGAAGGACAAGAAGAAACATTCGAGCAAAAATTTTTGCGACGTAATAAG  <  USA300TCH1516_ALE/2748555‑2748466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.