| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | USA300TCH1516_ALE | = 278640 | 178 (0.870) | 24 (0.130) | 8/140 | NT | 13.2% | intergenic (+235/+79) | USA300HOU_RS01215/gsiB | hypothetical protein/Glutathione‑binding protein GsiB |
| ? | USA300TCH1516_ALE | = 278661 | 161 (0.900) | intergenic (+256/+58) | USA300HOU_RS01215/gsiB | hypothetical protein/Glutathione‑binding protein GsiB | |||||
Only 100 of 272 total aligned reads displayed.
GTAATAATCATTTTATTGAAATTCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAATCGTTGTTCGATAATCGATTA > USA300TCH1516_ALE/278571‑278750 | gattgaacgataagggagtgtgggacattgatttatgtctcagccctgcttcaactataaAAACCACGCCATAATCATATTGGATGACTTc > 2:1769549‑M1/61‑91 (MQ=255)gattgaacgataagggagtgtgggacattgatttatgtctcagccctgcttcaactataaAAACCACGCCATAATCATATTGGATGACTTc > 2:2156490‑M1/61‑91 (MQ=255)gTAATAATCATTTTATTGAAATTCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTc > 2:3163529/1‑91 (MQ=255)gTAATAATCATTTTATTGAAATTCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTc < 2:492568/91‑1 (MQ=255) taataaTCATTTTATTGAAATTCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCg < 1:3890859‑M2/91‑2 (MQ=255) taataaTCATTTTATTGAAATTCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCg > 1:563034‑M2/1‑90 (MQ=255) attgaacgataagggagtgtgggacattgatttatgtctcagccctgcttcaactataaAAACCACGCCATAATCATATTGGATGACTTCg > 2:728669‑M1/60‑91 (MQ=255) ttgaacgataagggagtgtgggacattgatttatgtctcagccctgcttcaactataaAAACCACGCCATAATCATATTGGATGACTTCgg > 1:3774277‑M1/59‑91 (MQ=255) aataatCATTTTATTGAAATTCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCgg > 1:2326936‑M2/1‑89 (MQ=255) ataatCATTTTATTGAAATTCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCggc > 2:986208‑M2/1‑88 (MQ=255) aatCATTTTATTGAAATTCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCggcgt < 2:3530862‑M2/91‑6 (MQ=255) tCATTTTATTGAAATTCATCAAACAGAAATTAATACAATCATATCAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCggcgtgg > 2:3551719‑M2/1‑84 (MQ=255) tCATTTTATTGAAATTCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCggcgtgg > 2:3311397‑M2/1‑84 (MQ=255) tCATTTTATTGAAATTCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCggcgtgg > 2:2856799‑M2/1‑84 (MQ=255) tCATTTTATTGAAATTCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCggcgtgg > 1:868712‑M2/1‑84 (MQ=255) aTTTTATTGAAATTCATCAGACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCggcgtggtt < 2:3457548‑M2/91‑10 (MQ=255) aTTTTATTGAAATTCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCggcgtggtt < 1:2066889‑M2/91‑10 (MQ=255) aTTTTATTGAAATTCAGCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCggcgtggtt > 2:3781141‑M2/1‑82 (MQ=255) ttttATTGAAATTCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCggcgtggttt > 1:749468‑M2/1‑81 (MQ=255) ttttATTGAAATTCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCggcgtggttt > 1:1211939‑M2/1‑81 (MQ=255) ttATTGAAATTCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGttttt < 2:2122666/91‑1 (MQ=255) aTTGAAATTCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTtat > 1:1718017/1‑91 (MQ=255) tGAAATTCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAg < 2:911491/91‑1 (MQ=255) aaTTCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTg > 2:2037319/1‑91 (MQ=255) aaTTCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTg > 1:797256/1‑91 (MQ=255) aTTCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGa < 1:820069/91‑1 (MQ=255) ttCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGaa > 2:697079/1‑91 (MQ=255) catcaAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGc < 2:1725706/91‑1 (MQ=255) catcaAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGc < 1:924123/91‑1 (MQ=255) atcaAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCa > 1:1290854/1‑91 (MQ=255) caAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAgg < 1:3106393/91‑1 (MQ=255) caAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAgg < 1:2500682/91‑1 (MQ=255) aaaCAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAggg < 1:680708/91‑1 (MQ=255) aaaCAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAggg > 2:2556306/1‑91 (MQ=255) aaaCAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAggg > 2:3150299/1‑91 (MQ=255) aaaCAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAggg > 2:3390024/1‑91 (MQ=255) cAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTg < 2:1137408/91‑1 (MQ=255) cAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTg > 1:2795638/1‑91 (MQ=255) cAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTg > 1:1608148/1‑91 (MQ=255) aaTTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGAc > 1:509440/1‑91 (MQ=255) aaTTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGAc < 1:640936/91‑1 (MQ=255) aTTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACa < 2:2953968/91‑1 (MQ=255) aTTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACa < 1:1013380/91‑1 (MQ=255) ttAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACAt > 2:1274938/1‑91 (MQ=255) aaTACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATaa > 1:2244897/1‑91 (MQ=255) aaTACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATaa > 1:3172346/1‑91 (MQ=255) aaTACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTATTTATAGTTGAAGCAGGGCTGAGACATaa > 1:3172407/1‑91 (MQ=255) aTACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATaaa < 1:2467664/91‑1 (MQ=255) aCAACCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATc < 1:1333650/91‑1 (MQ=255) cAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCa > 2:1290676/1‑91 (MQ=255) tCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATg > 2:3128441/1‑91 (MQ=255) cATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGt < 2:2718652/91‑1 (MQ=255) tataAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTcc < 1:1856186/91‑1 (MQ=255) ataAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTccc > 1:1082313/1‑91 (MQ=255) ataAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTccc > 1:3840274/1‑91 (MQ=255) aaGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCcac > 2:3823023/1‑91 (MQ=255) aGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCcaca > 2:254163/1‑91 (MQ=255) gCATATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCacac < 1:3250614/91‑1 (MQ=255) gCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCacac < 1:455591/91‑1 (MQ=255) aaaTTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTc > 1:1462289/1‑91 (MQ=255) aaTTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTcc < 1:300029/91‑1 (MQ=255) ttAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCt > 1:1379412/1‑91 (MQ=255) AACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTAt < 1:2702798‑M1/91‑1 (MQ=255) AACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTAt < 1:2867‑M1/91‑1 (MQ=255) ACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATc > 2:1493318‑M1/1‑91 (MQ=255) ACCACGCCATAATCATATTGGATGACTTCGGCGTGGCTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATc < 2:3700032‑M1/91‑1 (MQ=255) ccgcGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTGGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCg < 2:578143/88‑1 (MQ=255) CCACGCCATAATCATATTGGATGACTTCGGCGTGGTTGTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCg < 1:3318310‑M1/91‑1 (MQ=255) cACGCCATAATCATATTGGATGACTTCggcgtggtttaatttgcttatatgattgtattaatttctgtttgatgaatttcaataaaatgat < 1:74886‑M2/91‑65 (MQ=255) cGCCATAATCATATTGGATGACTTCggcgtggtttaatttgcttatatgattgtattaatttctgtttgatgaatttcaataaaatgatta < 2:2428801‑M2/91‑67 (MQ=255) cGCCATAATCATATTGGATGACTTCggcgtggtttaatttgcttatatgattgtattaatttctgtttgatgaatttcaataaaatgatta < 2:1117546‑M2/91‑67 (MQ=255) GCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCa > 1:1000711‑M1/1‑91 (MQ=255) GCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCa > 1:962535‑M1/1‑91 (MQ=255) ccATAATCATATTGGACGACTTCggcgtggtttaatttgcttatatgattgtattaatttctgtttgatgaatttcaataaaatgattatt < 2:1248520‑M2/91‑69 (MQ=255) cATAATCATATTGGATGACTTCggcgtggtttaatttgcttatatgattgtattaatttctgtttgatgaatttcaataaaatgattatta < 2:2323631‑M2/91‑70 (MQ=255) cATAATCATATTGGATGACTTCggcgtggtttaatttgcttatatgattgtattaacttctgtttgatgaatttcaataaaatgattatta < 2:2992409‑M2/91‑70 (MQ=255) CATAATCATATTGGATGACTTCGGCGTGGTTTCTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAAt < 2:3879233‑M1/91‑1 (MQ=255) aTAATCATATTGGATGACTTCggcgtggtttaatttgcttatatgattgtattaatttctgtttgatgaatttcaataaaatgattattac < 1:1755168‑M2/91‑71 (MQ=255) aTAATCATATTGGATGACTTCGGCGCGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAATc > 2:1812092/1‑91 (MQ=255) tAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAATCg > 2:1602926/1‑91 (MQ=255) aaTCATATTGGATGACTTCggcgtggtttaatttgcttatatgattgtattaatttctgtttgatgaatttcaataaaatgattattacat < 2:796180‑M2/91‑73 (MQ=255) aaTCATATTGGATGACTTCggcgtggtttaatttgcttatatgattgtattaatttctgtttgatgaatttcaataaaatgattattacat < 2:796151‑M2/91‑73 (MQ=255) aTCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAATCgtt > 2:1706138/1‑91 (MQ=255) cATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAATCgttgt < 2:3365559/91‑1 (MQ=255) atTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAATCGTTGTTCg > 2:2112414/1‑91 (MQ=255) atTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAATCGTTGTTCg < 2:2353590/91‑1 (MQ=255) ttGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAATCGTTGTTCGa < 2:1932029/91‑1 (MQ=255) ggATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAATCGTTGTTCGATa < 1:3937592/91‑1 (MQ=255) ggATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAATCGTTGTTCGATa < 1:2208858/91‑1 (MQ=255) gATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACGTAAATCAATGTCCCACACTCCCTTATCGTTCAATCGTTGTTCGATaa < 1:3949008/91‑1 (MQ=255) gATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAATCGTTGTTCGATaa < 2:2661660/91‑1 (MQ=255) gATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAATCGTTGTTCGATaa < 2:3579572/91‑1 (MQ=255) gATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAATCGTTGTTCGATaa < 1:3526702/91‑1 (MQ=255) aTGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAATCGTTGTTCGATAAt < 1:1807683/91‑1 (MQ=255) aTGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAATCGTTGTTCGATAAt < 1:3647904/91‑1 (MQ=255) tGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAATCGTTGTTCGATAATc > 2:1098022/1‑91 (MQ=255) gACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAATCGTTGTTCGATAATCg < 1:1551502/91‑1 (MQ=255) gACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAATCGTTGTACGATAATCg > 1:3778381/1‑91 (MQ=255) aCTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAATCGTTGTTCGATAATCGa < 2:3060378/91‑1 (MQ=255) cTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAATCGTTGTTCGATAATCGAt > 1:3218601/1‑91 (MQ=255) | GTAATAATCATTTTATTGAAATTCATCAAACAGAAATTAATACAATCATATAAGCAAATTAAACCACGCCATAATCATATTGGATGACTTCGGCGTGGTTTTTATAGTTGAAGCAGGGCTGAGACATAAATCAATGTCCCACACTCCCTTATCGTTCAATCGTTGTTCGATAATCGATTA > USA300TCH1516_ALE/278571‑278750 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |