New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE 418185 =170 (1.000)5 (0.030) 4/280 13.6 2.8% intergenic (+38/+48) ychF/USA300HOU_RS01930 Ribosome‑binding ATPase YchF/hypothetical protein
?USA300TCH1516_ALE 418213 = 186 (1.160)intergenic (+66/+20) ychF/USA300HOU_RS01930 Ribosome‑binding ATPase YchF/hypothetical protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 193 total aligned reads displayed.

AAGAAGCGGGCAGACAACGATTAGAAGGTAAAGAATATATTATGCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCTTTGCACAATAAAGCCATTCAGGATGAAT  >  USA300TCH1516_ALE/418065‑418361
                                                                                                                                                    |                                                                                                                                                    
aagaagCGGGCAGACCACGATTAGAAGGTAAAGAATATATTATGCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCttt                                                                                                                                                      <  1:599377/149‑1 (MQ=255)
    agCGGGCAGACAACGATTAGAAGGTAACGAAGACAATATGCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAAt                                                                                                                                                  >  2:1657326/1‑149 (MQ=255)
    agCGGGCAGACAACGATTAGAAGGTAAAGAATATATTATGCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAAt                                                                                                                                                  >  2:1638986/1‑149 (MQ=255)
        ggCAGACAACGATTAGAAGGTAAAGAATATATTATGCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATg                                                                                                                                              <  2:954015/149‑1 (MQ=255)
           aGACAACGATTAGAAGGTAAAGAATATATTATGCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGt                                                                                                                                           <  2:337482/149‑1 (MQ=255)
           aGACAACGATTAGAAGGTAAAGAATATATTATGCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTATTCCAACTATTCGt                                                                                                                                           <  2:1488602/149‑1 (MQ=255)
           aGACAACGATTAGAAGGTAAAGAATATATTATGCAAGATGGCGATATCGGTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGt                                                                                                                                           <  1:1009519/149‑1 (MQ=255)
                atgATTAGAAGGTAAAGAAGATATTATGCAAGGTGGCGATAGCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAAt                                                                                                                                      <  1:352992/147‑1 (MQ=255)
                aCGATTAGAAGGTAAAGAATATATTATGCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAAt                                                                                                                                      >  2:1061580/1‑149 (MQ=255)
                aCGATTAGAAGGTAAAAAATATATTATGCAAGATGGCGATATCGTTAATTTAAGATTTGATGTATAAACGAAAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAAATATGCGTATAAt                                                                                                                                      >  2:1270561/1‑149 (MQ=255)
                 cGATTAGAAGGTAAAGAATATATTATGCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAAtt                                                                                                                                     <  1:1411005/149‑1 (MQ=255)
                 cGATTAGAAGGTAAAGAATATATTATGCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAAtt                                                                                                                                     <  2:217305/149‑1 (MQ=255)
                 cGATTAGAAGGTAAAGAATATATTATGCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAAtt                                                                                                                                     <  1:607458/149‑1 (MQ=255)
                 cGATAAGAAAGGAAAGAATATATTGAGGAAGAGTGCGATATCGTTGATTTCCAATTTAATGTATAAAAGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAAtt                                                                                                                                     <  1:392081/149‑1 (MQ=255)
                   aTTAGAAGGTAAAGAATATATTATGCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATtta                                                                                                                                   <  1:958058/149‑1 (MQ=255)
                         aGGTAAAGAATATATTATGCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTaaaa                                                                                                                             <  1:1424661/149‑1 (MQ=255)
                             aaaGAATATATTATGCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAAt                                                                                                                         <  1:1435611/149‑1 (MQ=255)
                              gaGAATATATTATGCAAGATGGCGATATCGTTCATTCCAGATTTAATGTGTAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGTCGGAATCAATTGTTCGCCGCTTTTAATTATGCGTATAATTAATTAAAAGAATg                                                                                                                        <  2:1047730/148‑1 (MQ=255)
                              aaGAATATATTATGCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATg                                                                                                                        <  2:1126640/149‑1 (MQ=255)
                              aaGAATATATTATGCAAGATGGCGATATCGTTCATTTCAGATTTAATGGATGAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGTAATCAATTGGTCGCCTCTTTTAAATATGCGTATAATTTATTAAAAGAATg                                                                                                                        <  2:450613/149‑1 (MQ=255)
                              aaGAATATATTAGGCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATg                                                                                                                        <  2:745045/149‑1 (MQ=255)
                                gAATATATTATGCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGa                                                                                                                      <  2:177983/149‑1 (MQ=255)
                                  atatatTATGCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATCCGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATACGTATAATTTATTAAAAAAATGGaat                                                                                                                    >  1:482002/1‑149 (MQ=255)
                                      attatGCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAAtt                                                                                                                <  2:942379/149‑1 (MQ=255)
                                        tatGCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAAtttt                                                                                                              >  1:466473/1‑149 (MQ=255)
                                           gCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGCGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATCATTTTACt                                                                                                           >  2:830288/1‑149 (MQ=255)
                                           gCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACt                                                                                                           >  1:444376/1‑149 (MQ=255)
                                           gCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATATAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAAAATGGAATAATTTTACt                                                                                                           >  2:99134/1‑149 (MQ=255)
                                            cAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTc                                                                                                          >  1:699273/1‑149 (MQ=255)
                                            cAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTc                                                                                                          >  1:1287413/1‑149 (MQ=255)
                                            cAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTc                                                                                                          >  1:1406261/1‑149 (MQ=255)
                                             aaGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAACTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTcc                                                                                                         >  1:67516/1‑148 (MQ=255)
                                              aGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTcgc                                                                                                        >  2:1262924/1‑149 (MQ=255)
                                               gATGGGGATATCGTTCATTTCAGATTTAATGCAAAAACGATAGAGTGAAGTTAATTAAATAGTATAAATGTAGACGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTcgcg                                                                                                       <  1:109815/149‑1 (MQ=255)
                                                            ttCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCtt                                                                                          >  1:264181/1‑149 (MQ=255)
                                                            ttCATTTCAGATTTAATGTATAAACGATAGAGGGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCtt                                                                                          >  1:1673137/1‑149 (MQ=255)
                                                              cATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGAGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGa                                                                                        >  2:307943/1‑149 (MQ=255)
                                                                 ttCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTg                                                                                     >  1:840917/1‑149 (MQ=255)
                                                                   cAGATTTAATGTATAAACGATAGAGAGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGATTGCt                                                                                   >  2:1008750/1‑149 (MQ=255)
                                                                        ttAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGaaaa                                                                              >  1:285006/1‑149 (MQ=255)
                                                                             gTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGt                                                                         <  2:66424/149‑1 (MQ=255)
                                                                             gTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGt                                                                         <  2:554727/149‑1 (MQ=255)
                                                                             gTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAAGGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGt                                                                         <  2:270636/149‑1 (MQ=255)
                                                                                taAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTg                                                                      >  1:483867/1‑149 (MQ=255)
                                                                                 taaCGCTAAAGTTAAGTTAATTAAATAGTATATATGTAGAAGCGGCGTAATCAATTGTTCGGCTCTTTTAATTATGCGTATAATTTAATAAAAGAATGGAATAATTTTTCTCGCCTTAAGAATATCCTGAGTGCTGAAAAATCGTTTGc                                                                     <  2:1398015/148‑1 (MQ=255)
                                                                                 aaaCGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGc                                                                     >  2:152272/1‑149 (MQ=255)
                                                                                 aaaCGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGc                                                                     >  1:63943/1‑149 (MQ=255)
                                                                                    cGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCtt                                                                  <  1:1547612/149‑1 (MQ=255)
                                                                                           gTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGt                                                           <  2:1372215/149‑1 (MQ=255)
                                                                                            tGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGta                                                          >  2:894929/1‑149 (MQ=255)
                                                                                             gAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGtaa                                                         >  2:224738/1‑148 (MQ=255)
                                                                                                  taattaaATAGTCTATATGTAGAAGAGGCGGAATCAATTGATCGACTCCTTTAATTATGCGTATAATTTATTAAAAGAATGGAACAATGGTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCa                                                    <  1:1430069/149‑1 (MQ=255)
                                                                                                  taattaaATAGTATATATGTAGAAGAGGCAGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAAAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCa                                                    <  1:235050/149‑1 (MQ=255)
                                                                                                  taattaaATAATAGATATGTAGAAGAGGCGGAAACAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCa                                                    <  1:774257/149‑1 (MQ=255)
                                                                                                  taatgaaATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATTCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCa                                                    <  2:85919/149‑1 (MQ=255)
                                                                                                     ttaaATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAGTATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGc                                                 >  2:499578/1‑149 (MQ=255)
                                                                                                           aGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTaaa                                           >  2:382993/1‑149 (MQ=255)
                                                                                                              atatatGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATACTTTTACTCGCGTTAATAATACCTTGAGTGCTGAAAAATTGTTTGCCATAGCCAGTATAAGCATGCTCTacaaca                                        >  1:934546/1‑149 (MQ=255)
                                                                                                                 tatGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACgaga                                     >  1:506607/1‑149 (MQ=255)
                                                                                                                  atGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACACGAt                                    >  1:684868/1‑149 (MQ=255)
                                                                                                                     tAGAATAGGCCGAAGCAATTGTTCGCCTCTTTTAATTATGCGTATAACTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAg                                 <  2:1330084/149‑1 (MQ=255)
                                                                                                                     tAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATATTATTGTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTACAACAAGATTAg                                 >  1:823294/1‑149 (MQ=255)
                                                                                                                     tAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAGCAAGAtttg                                 >  1:1129183/1‑147 (MQ=255)
                                                                                                                     tAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACa                                        <  1:125571/142‑1 (MQ=255)
                                                                                                                     tAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAg                                 >  2:257699/1‑149 (MQ=255)
                                                                                                                     tAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAg                                 >  2:1348162/1‑149 (MQ=255)
                                                                                                                     tAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAg                                 >  2:925117/1‑149 (MQ=255)
                                                                                                                     tAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAg                                 >  1:51558/1‑149 (MQ=255)
                                                                                                                     gagaagaGGCGGAATCAATTGTTCGCCTCTTGTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAg                                 <  2:1216477/148‑1 (MQ=255)
                                                                                                                      agaagaGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTCCTCGCGTTAATAATATCTTGAGTGCTGAAAACTTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGAGTAGc                                >  1:1577531/1‑149 (MQ=255)
                                                                                                                      agaagaGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGc                                >  2:143664/1‑149 (MQ=255)
                                                                                                                      agaagaGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGc                                >  1:1523650/1‑149 (MQ=255)
                                                                                                                       gaagaGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAGCAAGATTAGcc                               >  2:705815/1‑149 (MQ=255)
                                                                                                                        aagaGGCGGAATCAATTGTTCGCCTCTTTTAATTATGTGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCCAAACAACTGGTGTCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCt                              >  1:560209/1‑149 (MQ=255)
                                                                                                                        aagaGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAGACAAGATTAGCCt                              >  1:1503541/1‑149 (MQ=255)
                                                                                                                        aagaGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCt                              >  1:550349/1‑149 (MQ=255)
                                                                                                                        aagaGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCt                              >  1:220827/1‑149 (MQ=255)
                                                                                                                        aagaGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCGCTCAACCAAGATTAGCCt                              >  1:1471915/1‑149 (MQ=255)
                                                                                                                         agagGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCtt                             >  1:1049003/1‑149 (MQ=255)
                                                                                                                           agGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCTTTg                           >  1:370857/1‑149 (MQ=255)
                                                                                                                           agGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCTTTg                           >  1:322432/1‑149 (MQ=255)
                                                                                                                           agGCGGAATCAAATGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAATATAAGCAGGCTCTAAAACAAGATTAGCCTTTg                           >  1:1169957/1‑149 (MQ=255)
                                                                                                                            ggCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCTTTGc                          <  2:1479716/149‑1 (MQ=255)
                                                                                                                              cGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCTTTGcac                        >  2:169026/1‑149 (MQ=255)
                                                                                                                              cGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCTGTGcac                        >  1:418887/1‑149 (MQ=255)
                                                                                                                              cGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTACCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCTTTGcac                        >  1:539454/1‑149 (MQ=255)
                                                                                                                                 aaTCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCTTTGCACAAt                     >  1:981712/1‑149 (MQ=255)
                                                                                                                                   tCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCTTTGCACAATaa                   >  2:1239484/1‑149 (MQ=255)
                                                                                                                                   tCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCTTTGCACAATaa                   <  1:1638986/149‑1 (MQ=255)
                                                                                                                                   tCAATTGTTCGCCTCTTGTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCTTTGCACAATaa                   <  1:1657326/149‑1 (MQ=255)
                                                                                                                                   tCAAGTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTGCTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCTTTGCACAATaa                   <  2:482002/149‑1 (MQ=255)
                                                                                                                                    cAATTGTTCGCCTCTTTTAATTATGCGAATAAATGGTGGAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGAATAGCCATTGCACaaaaaa                  >  2:794099/1‑149 (MQ=255)
                                                                                                                                          ttCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCTTTGCACAATAAAGCCAtt            >  1:1229303/1‑149 (MQ=255)
                                                                                                                                          ttCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCTTTGCACAATAAAGCCAtt            >  1:70677/1‑149 (MQ=255)
                                                                                                                                          ttCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCTTTGCACAATAAAGCCAtt            >  1:1482572/1‑149 (MQ=255)
                                                                                                                                          ttCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCTTTGCACAATAAAGCCAtt            >  1:1419518/1‑149 (MQ=255)
                                                                                                                                          ttCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCTTTGCACAATAAAGCCAtt            >  1:1437498/1‑149 (MQ=255)
                                                                                                                                           tCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCTTTGCCCAATAAAGCCATTc           >  1:107290/1‑149 (MQ=255)
                                                                                                                                                  ttttAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCTTTGCACAATAAAGCCATTCAGGATGa    <  1:174330/149‑1 (MQ=255)
                                                                                                                                                  ttttAAGTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCTTTGCACAATAAAGCCATTCAGGATGa    <  1:1591621/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AAGAAGCGGGCAGACAACGATTAGAAGGTAAAGAATATATTATGCAAGATGGCGATATCGTTCATTTCAGATTTAATGTATAAACGATAGAGTGAAGTTAATTAAATAGTATATATGTAGAAGAGGCGGAATCAATTGTTCGCCTCTTTTAATTATGCGTATAATTTATTAAAAGAATGGAATAATTTTACTCGCGTTAATAATATCTTGAGTGCTGAAAAATTGTTTGCCTTCGCCAGTATAAGCAGGCTCTAAAACAAGATTAGCCTTTGCACAATAAAGCCATTCAGGATGAAT  >  USA300TCH1516_ALE/418065‑418361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.