New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE 633406 =116 (0.890)6 (0.050) 6/282 8.3 4.8% intergenic (+130/‑414) proP/yhfT Proline/betaine transporter/putative acyl‑‑CoA ligase YhfT
?USA300TCH1516_ALE 633436 = 128 (1.030)intergenic (+160/‑384) proP/yhfT Proline/betaine transporter/putative acyl‑‑CoA ligase YhfT
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 117 total aligned reads displayed.

GTAAGAATTAGAGATTTTAATAAAAAGTATAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACT  >  USA300TCH1516_ALE/633266‑633553
                                                                                                                                            |                                                                                                                                                   
gTAAGAATTAGAGATTTTAATAAAAAGTATAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACt                                                                                                                                              <  2:1096471/148‑1 (MQ=255)
 tAAGAATTAGAGATTTTAATAAAAAGTATAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTg                                                                                                                                             >  1:545184/1‑148 (MQ=255)
 tAAGAATTAGAGATTTTAATAAAAAGTATAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTg                                                                                                                                             >  2:1401886/1‑148 (MQ=255)
 tAAGAATTAGAGATTTTAATAAAAAGTATAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTg                                                                                                                                             >  1:1134603/1‑148 (MQ=255)
 tAAAAATTAGAGATTTTAATAAAAAGTATAAATCAATCGTCTATAGGTAATTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTg                                                                                                                                             <  1:1321016/148‑1 (MQ=255)
  aaGAATTAGAGATTTTAATAAAAAGTATAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGc                                                                                                                                            <  2:106662/148‑1 (MQ=255)
      aTTAGAGATTTTAATAAAAAGTATAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATg                                                                                                                                        <  2:393400/148‑1 (MQ=255)
          gagaTTTTAATAAAAAGTATAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTa                                                                                                                                    <  2:1395891/148‑1 (MQ=255)
          gagaTTTTAATAAAAAGTATAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTa                                                                                                                                    <  1:926815/148‑1 (MQ=255)
           agaTTTTAATAAAAAGTATAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTAt                                                                                                                                   >  2:1138530/1‑148 (MQ=255)
           agaTTTTAATAAAAAGTATAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTGTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTAt                                                                                                                                   <  2:1205137/148‑1 (MQ=255)
           agaTTGTAATAAAAAGTATAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGCGAACTGTAATTAGCCGTAATAACGTTTTTGTATATACATAAACCCCCACTGCAATGATTAt                                                                                                                                   <  2:859088/148‑1 (MQ=255)
                   ataaAAAGTATAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATgg                                                                                                                           >  2:292399/1‑148 (MQ=255)
                   ataaAAAGTATAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTATAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATgg                                                                                                                           <  2:713490/148‑1 (MQ=255)
                   ataaAAAGTATAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTATGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCTACTGCAATGATTATCGCAATgg                                                                                                                           <  2:961727/148‑1 (MQ=255)
                   ataaAAAATATAAACCAATCGTATCTAAGAACTTTAAAGATAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATACAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCCATgg                                                                                                                           >  2:354700/1‑148 (MQ=255)
                    taaAAAGTATAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATggg                                                                                                                          <  1:1021022/148‑1 (MQ=255)
                     aaaaaGTATAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATgggg                                                                                                                         >  1:1154120/1‑148 (MQ=255)
                     aaaaaGTATAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATgggg                                                                                                                         <  1:341995/148‑1 (MQ=255)
                             taAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATCAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAgg                                                                                                                 >  1:1241943/1‑148 (MQ=255)
                             taAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAGTGGGGGGAagatg                                                                                                                 >  1:581026/1‑146 (MQ=255)
                               aatcaatcGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAgggg                                                                                                               >  2:245193/1‑148 (MQ=255)
                               aatcaatcGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAgggg                                                                                                               >  2:245471/1‑148 (MQ=255)
                                atcaatcGTATATATGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGa                                                                                                              <  1:868067/148‑1 (MQ=255)
                                atcaatcGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATAAGCCGTAATAAAGTTTTTGTATATACATAAACCCCAACTGCAATGATTATACCACTGGGGGAAAGAgaggc                                                                                                              >  2:741954/1‑147 (MQ=255)
                                      cGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTaaa                                                                                                        <  1:734014/148‑1 (MQ=255)
                                       gTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAg                                                                                                       <  2:1277832/148‑1 (MQ=255)
                                       gTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACGGTAATTAGCCGTAATAAAGTTTTCGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAg                                                                                                       <  2:738034/148‑1 (MQ=255)
                                        tatataAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGc                                                                                                      <  2:401460/148‑1 (MQ=255)
                                         atataAGCCCTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTACAGCa                                                                                                     <  2:171807/148‑1 (MQ=255)
                                             aaGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGCCTTAAAACATATg                                                                                                 >  1:1070984/1‑148 (MQ=255)
                                              aGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGt                                                                                                >  2:599995/1‑148 (MQ=255)
                                               gCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGtt                                                                                               >  1:293397/1‑148 (MQ=255)
                                               gCACTTTAAAGCTAGTAGGTTCTGCTAAATTTAAAGAGCTTTTTAAATTGAGAACTGTAATTTGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGtt                                                                                               >  1:911312/1‑148 (MQ=255)
                                                cACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGACACGTCATTCACTGTAATAACGTTTGGTTCAATTCATAGAACCCTACTGCAGTGATTATCGCTATGGGGGAAAGAGGGGAGTTAAAGTATGTGttt                                                                                              >  1:1030803/1‑148 (MQ=255)
                                                 aCTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTa                                                                                             >  1:801875/1‑148 (MQ=255)
                                                       aaGCGAGGAGGTTCTGCTAACTTGAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATCCATAAACCCCCACTGCAATGATTATCGCAATGGGGTAAGGAGGGGACTTAAAGCATATGTTTAGCTTTg                                                                                       <  2:221861/148‑1 (MQ=255)
                                                        aGCTAGTAGGTTCTGCTAACTTTACAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGa                                                                                      >  1:702825/1‑148 (MQ=255)
                                                        aGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACTCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGa                                                                                      <  1:116430/148‑1 (MQ=255)
                                                        aGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGa                                                                                      <  2:782862/148‑1 (MQ=255)
                                                        aGCTAGGAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATGATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGa                                                                                      <  2:1077929/148‑1 (MQ=255)
                                                              tagGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACtt                                                                                >  1:557820/1‑148 (MQ=255)
                                                              tagGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACtt                                                                                >  2:521131/1‑148 (MQ=255)
                                                              tagGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACACCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACtt                                                                                >  2:1206385/1‑148 (MQ=255)
                                                               agGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGGTTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTa                                                                               >  1:385450/1‑148 (MQ=255)
                                                               agGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTa                                                                               >  2:257243/1‑148 (MQ=255)
                                                                      gCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATtct                                                                        <  1:1057291/148‑1 (MQ=255)
                                                                      gCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATtct                                                                        <  1:1056723/148‑1 (MQ=255)
                                                                      gCTAACTTTAAAGGGCTTTTTAAATTGAGAACTGTAATTAGTCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATtct                                                                        <  1:415052/148‑1 (MQ=255)
                                                                       cTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAACCCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATtctc                                                                       <  2:669845/148‑1 (MQ=255)
                                                                       cTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTTCGTCATATGTTTAGCTTTGAATACTTAAAATtctc                                                                       <  1:1036482/148‑1 (MQ=255)
                                                                       cTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGTATACTTAAAATtctc                                                                       <  1:883986/148‑1 (MQ=255)
                                                                        tAACTTTAAAGTGCATTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTctct                                                                      >  2:954432/1‑148 (MQ=255)
                                                                         aaCTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCtt                                                                     >  1:511191/1‑148 (MQ=255)
                                                                           cTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGc                                                                   <  1:245471/148‑1 (MQ=255)
                                                                           cTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGc                                                                   <  1:245193/148‑1 (MQ=255)
                                                                           cTTCAAAGTGCTTTTTAAATCGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGc                                                                   <  1:119200/148‑1 (MQ=255)
                                                                            tttAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCt                                                                  >  1:256118/1‑148 (MQ=255)
                                                                               aaaGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTAtt                                                               >  1:1155487/1‑148 (MQ=255)
                                                                               aaaGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTAtt                                                               >  1:1405258/1‑148 (MQ=255)
                                                                                  gTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGaa                                                            <  2:1401672/148‑1 (MQ=255)
                                                                                  gTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGaa                                                            <  2:1401556/148‑1 (MQ=255)
                                                                                  gTGCTTGTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTAGATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGaa                                                            <  2:66670/148‑1 (MQ=255)
                                                                                   tGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGaaa                                                           <  1:1104757/148‑1 (MQ=255)
                                                                                    gCTTTTTAATTTTTGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCCATGGGGGAAAGAGGGTATTTAAAGCATATGTTTAGCTTTGAATACTTCAAATTCTCTTGCTATTGAAAt                                                          >  2:784103/1‑148 (MQ=255)
                                                                                    gCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTgccat                                                          >  1:1012099/1‑144 (MQ=255)
                                                                                    gCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAAt                                                          >  2:1235267/1‑148 (MQ=255)
                                                                                    gCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAAt                                                          >  1:1145577/1‑148 (MQ=255)
                                                                                       ttttAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGtt                                                       >  1:828302/1‑148 (MQ=255)
                                                                                        tttAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATTTACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTa                                                      <  1:955892/148‑1 (MQ=255)
                                                                                        tttAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCTATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTa                                                      <  2:1205016/148‑1 (MQ=255)
                                                                                        tttAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTa                                                      <  1:1132851/148‑1 (MQ=255)
                                                                                         ttAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCTCAATGGGGGAAAGAGGGTACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAg                                                     <  1:1344886/148‑1 (MQ=255)
                                                                                         ttAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAg                                                     <  2:1135499/148‑1 (MQ=255)
                                                                                           aaaTTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATACACCCCGTCTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGa                                                   <  1:1093457/148‑1 (MQ=255)
                                                                                                   aaCTGCAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGTACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAata                                           <  2:140050/148‑1 (MQ=255)
                                                                                                     cTGTACTTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTTAAGCATATGTTTAGCTTTGAATACTTAAAACTCTCTTGCTATTGAAATGTTAGGATGTaataact                                         <  2:873632/148‑6 (MQ=255)
                                                                                                         aaTTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCCTATTTTGAGCGCAGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCtt                                     >  1:1385425/1‑148 (MQ=255)
                                                                                                              gCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGt                                >  2:63246/1‑148 (MQ=255)
                                                                                                                cGTAATAAAGTTTTTGTATATACATAAAGCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTAt                              >  1:881778/1‑148 (MQ=255)
                                                                                                                     taaAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGt                         >  1:449426/1‑148 (MQ=255)
                                                                                                                              tGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCaa                >  1:1204115/1‑148 (MQ=255)
                                                                                                                              tGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCaa                <  2:1161558/148‑1 (MQ=255)
                                                                                                                               gTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAAt               >  2:824721/1‑148 (MQ=255)
                                                                                                                                 atataCATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTa             <  2:292670/148‑1 (MQ=255)
                                                                                                                                  tataCATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTaa            >  1:751259/1‑148 (MQ=255)
                                                                                                                                  tataCATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGAACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCGATTGAAATGTTAGGACGTATATATATCTTAGAGTATTTTGTCCAACGCAATTaa            >  2:21219/1‑148 (MQ=255)
                                                                                                                                     aCATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAatat         >  2:150700/1‑148 (MQ=255)
                                                                                                                                      cATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTCGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTCTTTTGTCCAACGCAATTAATAtt        >  2:911479/1‑148 (MQ=255)
                                                                                                                                       aTAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTg       <  1:1271343/148‑1 (MQ=255)
                                                                                                                                       aTAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTg       >  2:1110908/1‑148 (MQ=255)
                                                                                                                                       aTAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTg       >  1:429359/1‑148 (MQ=255)
                                                                                                                                        tAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGATGGGACTTAAAGCATAAGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTGTTAGAGTATTTTGTCCAATGAAATTAATTTTga      >  2:741963/1‑148 (MQ=255)
                                                                                                                                         aaaCCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCTATTAATATTgag     <  1:963150/148‑1 (MQ=255)
                                                                                                                                           aCCCCCACTGCAATTATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGAc   >  1:1107655/1‑148 (MQ=255)
                                                                                                                                           aCCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGCAATGTTTGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGAc   >  1:895814/1‑148 (MQ=255)
                                                                                                                                           aCCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGAc   >  1:218020/1‑148 (MQ=255)
                                                                                                                                           aCCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGAc   >  1:30529/1‑148 (MQ=255)
                                                                                                                                           aCCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGAc   >  2:1025441/1‑148 (MQ=255)
                                                                                                                                           aCCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGAc   >  2:374409/1‑148 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
GTAAGAATTAGAGATTTTAATAAAAAGTATAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACT  >  USA300TCH1516_ALE/633266‑633553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.