New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE 633406 =116 (0.890)6 (0.050) 6/282 8.3 4.8% intergenic (+130/‑414) proP/yhfT Proline/betaine transporter/putative acyl‑‑CoA ligase YhfT
?USA300TCH1516_ALE 633436 = 128 (1.030)intergenic (+160/‑384) proP/yhfT Proline/betaine transporter/putative acyl‑‑CoA ligase YhfT
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 132 total aligned reads displayed.

TAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCTTCAATATTATTATAGAGAA  >  USA300TCH1516_ALE/633295‑633579
                                                                                                                                             |                                                                                                                                               
taAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATCAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAgg                                                                                                                                           >  1:1241943/1‑148 (MQ=255)
 aaaTCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAggg                                                                                                                                          >  2:832428/1‑148 (MQ=255)
  aatcaatcGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAgggg                                                                                                                                         >  2:245471/1‑148 (MQ=255)
   atcaatcGTATATATGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGa                                                                                                                                        <  1:868067/148‑1 (MQ=255)
   atcaatcGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATAAGCCGTAATAAAGTTTTTGTATATACATAAACCCCAACTGCAATGATTATACCACTGGGGGAAAGAgaggc                                                                                                                                        >  2:741954/1‑147 (MQ=255)
         cGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTaaa                                                                                                                                  <  1:734014/148‑1 (MQ=255)
          gTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAg                                                                                                                                 <  2:1277832/148‑1 (MQ=255)
          gTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACGGTAATTAGCCGTAATAAAGTTTTCGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAg                                                                                                                                 <  2:738034/148‑1 (MQ=255)
                aaGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGCCTTAAAACATATg                                                                                                                           >  1:1070984/1‑148 (MQ=255)
                 aGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGt                                                                                                                          >  2:599995/1‑148 (MQ=255)
                  gCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGtt                                                                                                                         >  1:293397/1‑148 (MQ=255)
                  gCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGtt                                                                                                                         >  2:1375734/1‑148 (MQ=255)
                   cACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGACACGTCATTCACTGTAATAACGTTTGGTTCAATTCATAGAACCCTACTGCAGTGATTATCGCTATGGGGGAAAGAGGGGAGTTAAAGTATGTGttt                                                                                                                        >  1:1030803/1‑148 (MQ=255)
                          aaGCGAGGAGGTTCTGCTAACTTGAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATCCATAAACCCCCACTGCAATGATTATCGCAATGGGGTAAGGAGGGGACTTAAAGCATATGTTTAGCTTTg                                                                                                                 <  2:221861/148‑1 (MQ=255)
                           aGCTAGTAGGTTCTGCTAACTTTACAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGa                                                                                                                >  1:702825/1‑148 (MQ=255)
                           aGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACTCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGa                                                                                                                <  1:116430/148‑1 (MQ=255)
                           aGCTAGGAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATGATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGa                                                                                                                <  2:1077929/148‑1 (MQ=255)
                                 tagGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACtt                                                                                                          >  1:557820/1‑148 (MQ=255)
                                 tagGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACtt                                                                                                          >  2:521131/1‑148 (MQ=255)
                                  agGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGGTTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTa                                                                                                         >  1:385450/1‑148 (MQ=255)
                                  agGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTa                                                                                                         >  2:257243/1‑148 (MQ=255)
                                         gCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATtct                                                                                                  <  1:1056723/148‑1 (MQ=255)
                                         gCTAACTTTAAAGGGCTTTTTAAATTGAGAACTGTAATTAGTCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATtct                                                                                                  <  1:415052/148‑1 (MQ=255)
                                          cTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAACCCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATtctc                                                                                                 <  2:669845/148‑1 (MQ=255)
                                          cTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTTCGTCATATGTTTAGCTTTGAATACTTAAAATtctc                                                                                                 <  1:1036482/148‑1 (MQ=255)
                                          cTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGTATACTTAAAATtctc                                                                                                 <  1:883986/148‑1 (MQ=255)
                                           tAACTTTAAAGTGCATTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTctct                                                                                                >  2:954432/1‑148 (MQ=255)
                                            aaCTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCtt                                                                                               >  1:511191/1‑148 (MQ=255)
                                              cTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGc                                                                                             <  1:245471/148‑1 (MQ=255)
                                              cTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGc                                                                                             <  1:245193/148‑1 (MQ=255)
                                              cTTCAAAGTGCTTTTTAAATCGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGc                                                                                             <  1:119200/148‑1 (MQ=255)
                                                ttAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTa                                                                                           >  1:162508/1‑148 (MQ=255)
                                                  aaaGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTAtt                                                                                         >  1:1062736/1‑148 (MQ=255)
                                                  aaaGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTAtt                                                                                         >  1:1155487/1‑148 (MQ=255)
                                                     gTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGaa                                                                                      <  2:1401672/148‑1 (MQ=255)
                                                       gCTTTTTAATTTTTGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCCATGGGGGAAAGAGGGTATTTAAAGCATATGTTTAGCTTTGAATACTTCAAATTCTCTTGCTATTGAAAt                                                                                    >  2:784103/1‑148 (MQ=255)
                                                       gCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTgccat                                                                                    >  1:1012099/1‑144 (MQ=255)
                                                       gCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAAt                                                                                    >  2:1235267/1‑148 (MQ=255)
                                                       gCTTTTTAAATTGAAAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAAt                                                                                    <  1:1226386/148‑1 (MQ=255)
                                                        cTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATg                                                                                   <  1:1138530/148‑1 (MQ=255)
                                                          ttttATATTGGGAACTGTAATTAGCCGTAATAAATTTTATATATATACGTAAGTCTCCACTGTAATTATTATCGCAATCGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTTTTGCTATTGAAATGtt                                                                                 <  2:427602/148‑1 (MQ=255)
                                                          ttttAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGtt                                                                                 >  1:828302/1‑148 (MQ=255)
                                                           tttAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATTTACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTa                                                                                <  1:955892/148‑1 (MQ=255)
                                                           tttAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCTATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTa                                                                                <  2:1205016/148‑1 (MQ=255)
                                                           tttAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTa                                                                                <  1:1132851/148‑1 (MQ=255)
                                                           tttAAAGTGAGAACTGGAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTa                                                                                <  2:164455/148‑1 (MQ=255)
                                                            ttAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCTCAATGGGGGAAAGAGGGTACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAg                                                                               <  1:1344886/148‑1 (MQ=255)
                                                            ttAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAg                                                                               <  2:1135499/148‑1 (MQ=255)
                                                              aaaTTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATACACCCCGTCTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGa                                                                             <  1:1093457/148‑1 (MQ=255)
                                                                      aaCTGCAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGTACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAata                                                                     <  2:140050/148‑1 (MQ=255)
                                                                        cTGTACTTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTTAAGCATATGTTTAGCTTTGAATACTTAAAACTCTCTTGCTATTGAAATGTTAGGATGTaataact                                                                   <  2:873632/148‑6 (MQ=255)
                                                                            aaTTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCCTATTTTGAGCGCAGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCtt                                                               >  1:1385425/1‑148 (MQ=255)
                                                                            aaTTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCtt                                                               >  1:788986/1‑148 (MQ=255)
                                                                                        taaAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGt                                                   >  1:449426/1‑148 (MQ=255)
                                                                                                 tGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCaa                                          <  2:1161558/148‑1 (MQ=255)
                                                                                                    atataCATAAACCCCCATTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAACTATGTCTTAGAGTATTTTGTCCAACGCAATTa                                       <  1:899607/148‑1 (MQ=255)
                                                                                                    atataCATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTa                                       <  2:292670/148‑1 (MQ=255)
                                                                                                     tataCATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGAACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCGATTGAAATGTTAGGACGTATATATATCTTAGAGTATTTTGTCCAACGCAATTaa                                      >  2:21219/1‑148 (MQ=255)
                                                                                                        aCATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAatat                                   >  2:150700/1‑148 (MQ=255)
                                                                                                          aTAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTg                                 >  2:1110908/1‑148 (MQ=255)
                                                                                                          aTAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTg                                 >  1:429359/1‑148 (MQ=255)
                                                                                                           tAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGATGGGACTTAAAGCATAAGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTGTTAGAGTATTTTGTCCAATGAAATTAATTTTga                                >  2:741963/1‑148 (MQ=255)
                                                                                                            aaaCCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCTATTAATATTgag                               <  1:963150/148‑1 (MQ=255)
                                                                                                              aCCCCCACTGCAATTATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGAc                             >  1:1107655/1‑148 (MQ=255)
                                                                                                              aCCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGCAATGTTTGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGAc                             >  1:895814/1‑148 (MQ=255)
                                                                                                              aCCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGAc                             >  1:30529/1‑148 (MQ=255)
                                                                                                              aCCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGAc                             >  1:218020/1‑148 (MQ=255)
                                                                                                              aCCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGAc                             >  2:374409/1‑148 (MQ=255)
                                                                                                                ccccACTGCAATGATTATCGCATTGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGActc                           >  2:957830/1‑148 (MQ=255)
                                                                                                                  ccACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTa                         <  1:139662/148‑1 (MQ=255)
                                                                                                                  ccACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTa                         <  1:832428/148‑1 (MQ=255)
                                                                                                                  ccACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTa                         <  2:511191/148‑1 (MQ=255)
                                                                                                                   cACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTaa                        >  1:793759/1‑148 (MQ=255)
                                                                                                                      tggaaTGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGTAATTAATATTGAGACTCTAACCt                     <  1:203883/145‑1 (MQ=255)
                                                                                                                       ggaaTGATTATCGCAATGGGGGAAAGAGGGGACTTGAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCtt                    <  2:70288/146‑1 (MQ=255)
                                                                                                                       gCAATGATTATCGCCTGGGGGTAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCtt                    <  2:271254/148‑1 (MQ=255)
                                                                                                                       gCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGGATATGTTTAGCTTTGAATACTTAATATTCTCTTGCTATTGAAATGTTAGGATGTAATTTTGTCTTAGAGTATTTTGTCCAACGCAATTAATGTTGAGTCTTTAACCtt                    <  2:324599/148‑1 (MQ=255)
                                                                                                                       gCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCtt                    <  2:801631/148‑1 (MQ=255)
                                                                                                                       gCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTGGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGCATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATCTTGAGACTCTAACCtt                    <  2:1039487/148‑1 (MQ=255)
                                                                                                                         aaTGATTATCGCGATGGCGGTAATATGGGACTGAAAGCATATGGGTAGTTTTGAATACTCAACATCCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCCTCa                  <  2:218728/148‑1 (MQ=255)
                                                                                                                         aaTGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCCTATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCTTCa                  >  1:200224/1‑148 (MQ=255)
                                                                                                                         aaTGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCTTCa                  <  1:544909/148‑1 (MQ=255)
                                                                                                                         aaTGATTATCGAAATGGGGGCAAGAGGGGACTTAAAGCATATGTTTAGCTTTGCATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTACAAGGACTTAAAAATTGAGACTCTAACCTTCa                  >  2:1344702/1‑148 (MQ=255)
                                                                                                                          aggATTATCGCAATGGGGGAAAGAGGGGACTTCAGACTTAGGTGTATCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCCACGCAATTGATTTTGAGACCCTCACCTTCac                 <  2:636691/146‑2 (MQ=255)
                                                                                                                          aTGATTATCGCAATGGGGGANANAGGNGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCTTCaa                 <  1:741954/148‑1 (MQ=255)
                                                                                                                          aTGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCTTCaa                 <  2:63452/148‑1 (MQ=255)
                                                                                                                           tGATTCTCGCAATGGGGGAAAGAGGGGACTTAAAGCAGATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCTTCAat                <  2:968237/148‑1 (MQ=255)
                                                                                                                           tGATTAGCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCTTCAat                <  2:808637/148‑1 (MQ=255)
                                                                                                                            gATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAATATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCTTCAata               <  2:1010821/148‑1 (MQ=255)
                                                                                                                            gATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCTTCAata               >  2:608776/1‑148 (MQ=255)
                                                                                                                             aTTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCTTCAatat              <  1:1208523/148‑1 (MQ=255)
                                                                                                                             aTTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTGTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCTTCAatat              <  2:843112/148‑1 (MQ=255)
                                                                                                                                agcGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCTTCAAtattat           <  2:137702/146‑1 (MQ=255)
                                                                                                                                  cGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCTTCAAtattatta         >  1:786341/1‑148 (MQ=255)
                                                                                                                                  cGCAATGGGGGAAAGAGGGGACTCAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCTTCAAtattatta         <  1:354700/148‑1 (MQ=255)
                                                                                                                                   gCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCTTCAAttttattat        >  2:483282/1‑148 (MQ=255)
                                                                                                                                        gggggAAAGAGGGGACTTAGAGCATATGTTTAGCTTTGAATACTTAAACTTCTATTGCTATTTTATTGTTAGGATTTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCTTCAATATTATTATAgaga   <  2:1205596/148‑1 (MQ=255)
                                                                                                                                         ggggAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGCGCATTTTATCCAACGCAAGTAATATTGAGACTCTAACCTTCAATATTATTATAGaaaa  >  2:639962/1‑148 (MQ=255)
                                                                                                                                         ggggAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCTTCAATATTCTTATAGCGaa  >  2:595781/1‑148 (MQ=255)
                                                                                                                                         ggggAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCTTCAATATTATTATAGAGaa  >  2:7019/1‑148 (MQ=255)
                                                                                                                                             |                                                                                                                                               
TAAATCAATCGTATATAAGCACTTTAAAGCTAGTAGGTTCTGCTAACTTTAAAGTGCTTTTTAAATTGAGAACTGTAATTAGCCGTAATAAAGTTTTTGTATATACATAAACCCCCACTGCAATGATTATCGCAATGGGGGAAAGAGGGGACTTAAAGCATATGTTTAGCTTTGAATACTTAAAATTCTCTTGCTATTGAAATGTTAGGATGTAAATATGTCTTAGAGTATTTTGTCCAACGCAATTAATATTGAGACTCTAACCTTCAATATTATTATAGAGAA  >  USA300TCH1516_ALE/633295‑633579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.