BRESEQ :: Evidence

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	= 1205323	102 (0.780)	25 (0.260) +AGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAG	8/218	6.1	24.9%	intergenic (‑204/‑365)	USA300HOU_RS15290/lspA	hypothetical protein/Lipoprotein signal peptidase
*	?	USA300TCH1516_ALE	= 1443739	NA (NA)	25 (0.260) +AGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAG	8/218	6.1	24.9%	intergenic (‑312/+41)	USA300TCH1516_01342/brnQ_3	hypothetical protein/Branched‑chain amino acid transport system 2 carrier protein
Rejected: Coverage evenness skew score above cutoff.

Only 100 of 103 total aligned reads displayed.

AATTTGTCCAATTAAGTGTAGACGATTCATGTCTTATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATAATTTTTAATTAGAAAATATCTTACTACTGTCTTTT  >  USA300TCH1516_ALE/1205176‑1205470

aaTTTGTCCAATTAAGTGTAGACGATTCATGTCTTATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGaa                                                                                                                                                     >  2:789934/1‑148 (MQ=255)

   ttGTCCAATTAAGTTTAGACGATTCATGTCTTATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAggg                                                                                                                                                  <  2:318782/148‑1 (MQ=255)

   gtGTCCCATTAAGTGTAAACGATGCACGTCTTATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAggg                                                                                                                                                  <  1:505653/147‑1 (MQ=255)

     gTCCAATTAAGTGTAGACGATTCATGTCTTATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAAGGAc                                                                                                                                                >  1:359245/1‑148 (MQ=255)

         aaTTAAGTGTAGACGATTCATGTCTTATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCcat                                                                                                                                            >  1:1042512/1‑148 (MQ=255)

         aaTTAAGTGTAGACGATTCATGTCTTATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCcat                                                                                                                                            <  2:974552/148‑1 (MQ=255)

         aaTTAAGTGTAGACGATTCATGTCTTATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCcat                                                                                                                                            >  2:805371/1‑148 (MQ=255)

         aaTTAAGTGTAGACGATTCATGTCTTATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCcat                                                                                                                                            >  1:704619/1‑148 (MQ=255)

         aaTTAAGTGTAGACGATTCATGTCTTATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCcat                                                                                                                                            >  1:454857/1‑148 (MQ=255)

         aaTTAAGTGTAGACGATTCATGTCGTATTTTTTTAAATTTTTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGCGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTCGGAAGGGACCcat                                                                                                                                            >  1:782980/1‑148 (MQ=17)

              aGTGTAGACGATTCATGTCTTATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTTAGACGGCACCCTAGGAAGGGACCCAtcatca                                                                                                                                       >  1:944854/1‑148 (MQ=255)

              aGTGTAGACGATTCATGTCTTATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCAtcatca                                                                                                                                       >  1:45801/1‑148 (MQ=255)

               gtgtAGACGATTCATGTCTTATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCaa                                                                                                                                      >  2:494164/1‑148 (MQ=255)

                   aGACGATTCATGTCTTATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAAt                                                                                                                                  >  1:193044/1‑148 (MQ=255)

                    gACGATTCATGTCTTATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAAtt                                                                                                                                 <  1:687906/148‑1 (MQ=255)

                    caCGACTCATGTCTGATATTTATAAAGTATTGTAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTTAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAAtt                                                                                                                                 <  2:735634/147‑1 (MQ=17)

                     aCGATTCGTGTCTTATTTTTTTAGAGAATTTAACAGTAAAATTACATGTTAATACGTAGTATTAATGGCGGGACGCCTGACGGAGCCGTGCCAGTCGAAGACCGAGGCTGAAACCGCACCCTAGGAAGGGACCCCTCATCCAAAATTc                                                                                                                                <  2:1222864/148‑1 (MQ=14)

                      cGATTCATGTCTTATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCt                                                                                                                               >  1:388559/1‑148 (MQ=255)

                       gATTCATGTCTTATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAATACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTa                                                                                                                              <  2:342481/148‑1 (MQ=255)

                        aTTCATGTCTTATTTTTTTAAAGCATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTAt                                                                                                                             <  2:1414168/148‑1 (MQ=255)

                          tCATGTCTTATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTAttt                                                                                                                           <  2:1251947/148‑1 (MQ=255)

                           cATGTCTTATTTTTTTAAAGAGTTTAAAAGTAACATAAGGTTCTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTta                                                                                                                          >  2:585127/1‑148 (MQ=17)

                             tGTCTTATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTtata                                                                                                                        <  1:789934/148‑1 (MQ=255)

                               tCTTATTTTTTTAAATTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGa                                                                                                                      >  2:1309388/1‑148 (MQ=255)

                               tCTTATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGCCCCATCATCAAAAATTCTATTACTAGa                                                                                                                      >  2:240120/1‑148 (MQ=255)

                                 ttATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAAt                                                                                                                    <  2:1365264/148‑1 (MQ=255)

                                      tttttAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTATTGGCGAGACTCCTGAGGGAGCAGTGTCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGTAGGGACCCACCATGAAAAATTCTATATATAGAATTTTAc                                                                                                               <  1:1038509/148‑1 (MQ=17)

                                      tttttAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTAc                                                                                                               <  1:995809/148‑1 (MQ=255)

                                         ttAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGt                                                                                                            <  2:1376340/148‑1 (MQ=255)

                                         ttAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGt                                                                                                            <  2:732488/148‑1 (MQ=255)

                                            aaGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGGCGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAAt                                                                                                         >  1:718515/1‑148 (MQ=255)

                                             aGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAggagtg                                                                                                        >  1:370252/1‑143 (MQ=255)

                                                aTTTAAAAGTAAAATTAGCTGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGc                                                                                                     <  2:401580/148‑1 (MQ=255)

                                                aTTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGc                                                                                                     <  2:375848/148‑1 (MQ=255)

                                                 tttAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGcc                                                                                                    <  1:1356853/148‑1 (MQ=255)

                                                  ttAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGGCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCa                                                                                                   >  1:766185/1‑148 (MQ=255)

                                                  ttAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCa                                                                                                   >  1:936933/1‑148 (MQ=255)

                                                  ttAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCa                                                                                                   >  1:280900/1‑148 (MQ=255)

                                                    aaaaGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGa                                                                                                 >  2:355029/1‑148 (MQ=255)

                                                     aaaGTAAAATTACATGTTAATAGGTAGTATTAATGGCGAGACTCCTGAGGGAGAAGTGCCAGTCGAAGAACGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGAt                                                                                                >  1:207758/1‑148 (MQ=255)

                                                      aaGGAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGAGAGAGCCGCCATTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATg                                                                                               <  1:323517/148‑1 (MQ=21)

                                                        gtgaaaCTACATGTTATTACGTAGTATTAATGGCGAGCCTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGTCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATggg                                                                                             <  2:943125/145‑1 (MQ=21)

                                                          aaaaTTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCa                                                                                           >  2:897103/1‑148 (MQ=255)

                                                           aaaTTACATGGTAATACGTAGTATTAATGGCGAGACTCCTGGGGGCGCAGTTCCAGTCGAAGACCGAGGCTGAGACGGCAACCTAGGAAGGTGCCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCAt                                                                                          <  2:1280572/148‑1 (MQ=21)

                                                                aCATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGa                                                                                     >  2:400970/1‑148 (MQ=255)

                                                                aCATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGa                                                                                     >  1:971668/1‑148 (MQ=255)

                                                                  aTGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAg                                                                                   >  2:438373/1‑148 (MQ=255)

                                                                   tGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACGGTAATGTGCCAGATGGGCATAGCGAAGc                                                                                  >  1:1270414/1‑148 (MQ=255)

                                                                    gTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGcc                                                                                 >  2:766687/1‑148 (MQ=255)

                                                                     ttAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCa                                                                                <  2:382163/148‑1 (MQ=255)

                                                                           cGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCGACTCAAt                                                                          >  2:762502/1‑148 (MQ=255)

                                                                                taTTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGaa                                                                     <  2:454857/148‑1 (MQ=255)

                                                                                taTTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGaa                                                                     <  1:805371/148‑1 (MQ=255)

                                                                                taTTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGaa                                                                     <  1:1258849/148‑1 (MQ=255)

                                                                                taTTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGaa                                                                     <  2:704619/148‑1 (MQ=255)

                                                                                 aTTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAg                                                                    >  2:1250163/1‑148 (MQ=255)

                                                                                 aTTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAg                                                                    >  2:1265794/1‑148 (MQ=255)

                                                                                  ttAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGt                                                                   <  1:222796/148‑1 (MQ=255)

                                                                                   tAATGGCGAGACTCCTGAGGTAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCGAGGAAGGGACCCATCAGCAAAAATTCTATTTATAGAATTTTACAGTAATTTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTa                                                                  <  2:147518/148‑1 (MQ=255)

                                                                                     aTGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTCTTTATAGAATTTTACAGTAATGTGCCAGATGGACATGGCGAAGCCATTCAATACGAAGTAtt                                                                >  1:222221/1‑148 (MQ=255)

                                                                                     aTGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTAtt                                                                <  2:465728/148‑1 (MQ=255)

                                                                                     aTGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTAtt                                                                <  1:1256763/148‑1 (MQ=255)

                                                                                      tGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTg                                                               >  1:183813/1‑148 (MQ=255)

                                                                                      tGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTg                                                               >  1:163477/1‑148 (MQ=255)

                                                                                      tGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTGTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTg                                                               >  1:959369/1‑148 (MQ=255)

                                                                                       ggCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGCTGGGCATAGCGAAGCCATTCAATACGAAGTATTGt                                                              <  1:138000/148‑1 (MQ=255)

                                                                                       ggCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGt                                                              <  1:496482/148‑1 (MQ=255)

                                                                                           agaCTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATaa                                                          <  1:738998/148‑1 (MQ=255)

                                                                                           agaCTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATaa                                                          <  2:109106/148‑1 (MQ=255)

                                                                                            gaCTCCTGAGGTAGCAGGCCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTCATGTTCCATATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATaaa                                                         <  1:893249/148‑1 (MQ=17)

                                                                                            gaCTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATaaa                                                         <  2:712228/148‑1 (MQ=255)

                                                                                            gaCTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATaaa                                                         <  2:602081/148‑1 (MQ=255)

                                                                                            gaCTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATaaa                                                         <  2:96605/148‑1 (MQ=255)

                                                                                            gaCGCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGTATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATaaa                                                         <  2:862332/148‑1 (MQ=255)

                                                                                                ccTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATaga                                                     >  1:664802/1‑148 (MQ=255)

                                                                                                  tGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAgaga                                                   <  1:1346220/148‑1 (MQ=255)

                                                                                                  tGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCAGCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAgaga                                                   >  2:862066/1‑148 (MQ=255)

                                                                                                     gggAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATCGAGAAca                                                <  2:247463/148‑1 (MQ=255)

                                                                                                     gggAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTGATAGAATGTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAAca                                                <  2:45801/148‑1 (MQ=255)

                                                                                                         gCAGTGCCAGTCGAAGACCGAGGCTGAGACCGCCCCCTAGCAAGGGACCCATCATCAAAAATTCTATTTATCGAATTTGACAGTGAGGGGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAAcagcag                                            <  2:572067/148‑1 (MQ=17)

                                                                                                            gtaccggTCGAAGCTCGAGGCTGAGACGGCACACTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATTAGCATAGCGAAGCCATTAAATACGAAGTATTGTATAAACAGAGAACAGCAGTaa                                         <  2:1028192/142‑1 (MQ=11)

                                                                                                             tGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAg                                        <  1:1144391/148‑1 (MQ=255)

                                                                                                             tGCCAGTAGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAg                                        <  1:766196/148‑1 (MQ=255)

                                                                                                                cAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGGCATTCATTACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATa                                     >  1:457175/1‑148 (MQ=255)

                                                                                                                    cGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGACGTATTGTATAAATAAAGAAAAGCAGTAAGATAAttt                                 >  2:1364248/1‑148 (MQ=255)

                                                                                                                    cGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATAAttt                                 >  2:470145/1‑148 (MQ=255)

                                                                                                                          ccGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATAATTTTTAAtt                           >  1:470982/1‑148 (MQ=255)

                                                                                                                           ggtagcggagaCGGCCCCCTAGGAAGGGACCCGTCATCAAAAATTCTTTCTATATAATTTGACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGCGAACATCAGTAAGATAATTTTCAATTa                          <  1:750193/141‑1 (MQ=11)

                                                                                                                             aGGCTGAGACTGCACTCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAAGGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATAATTTTTAATTAGa                        <  2:382351/148‑1 (MQ=255)

                                                                                                                                     aCGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATAATTTTTAATTAGAAAATATCt                <  1:1066675/148‑1 (MQ=255)

                                                                                                                                     aCGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATAATTTTTAATTAGAAAATATCt                <  1:1362531/148‑1 (MQ=255)

                                                                                                                                       ggCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATAATTTTTAATTAGAAAATATCTta              <  1:494164/148‑1 (MQ=255)

                                                                                                                                         cACCATAGGAAGGGACCCGTCATCCAAAATTCTATTTACAGAATTGTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACTAAGTATTGTATAAATAGAGAACAGCAGTAAGATAATTTTCAATTAGAAAATATCTtact            <  1:469487/148‑1 (MQ=14)

                                                                                                                                             cTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATAATTTTTAATTAGAAAATATCTTACTACTg        >  1:567042/1‑148 (MQ=255)

                                                                                                                                              tAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATAATTTTTAATTAGAAAATATCTTACTACTGt       <  2:1301417/148‑1 (MQ=255)

                                                                                                                                                ggAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATAATTTTTAATTAGAAAATATCTTACTACTGTCt     >  2:1125065/1‑148 (MQ=255)

                                                                                                                                                ggAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATAATTTTTAATTAGAAAATATCTTACTACTGTCt     >  2:1208965/1‑148 (MQ=255)

                                                                                                                                                  aaGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATAATTTTTAATTAGAAAATATCTTACTACTGTCttt   <  1:1045348/148‑1 (MQ=255)

                                                                                                                                                   aGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATAATTTTTAATTAGAAAATATCTTACTACTGTCtttt  <  2:1371139/148‑1 (MQ=255)

                                                                                                                                                   aGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATAATTTTTAATTAGAAAATATCTTACTACTGTCtttt  <  2:1369476/148‑1 (MQ=255)

AATTTGTCCAATTAAGTGTAGACGATTCATGTCTTATTTTTTTAAAGTATTTAAAAGTAAAATTACATGTTAATACGTAGTATTAATGGCGAGACTCCTGAGGGAGCAGTGCCAGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAGGGACCCATCATCAAAAATTCTATTTATAGAATTTTACAGTAATGTGCCAGATGGGCATAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATAATTTTTAATTAGAAAATATCTTACTACTGTCTTTT  >  USA300TCH1516_ALE/1205176‑1205470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.