New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	34173 =	73 (1.250)	4 (0.070)	3/254	NT	5.1%	coding (462/480 nt)	rlmH	Ribosomal RNA large subunit methyltransferase H
	?	USA300TCH1516_ALE	= 88831	77 (1.350)					intergenic (+204/‑561)	nusG_1/USA300TCH1516_00080	Transcription termination/antitermination protein NusG/hypothetical protein

AACTTATTATTGTTAAGGAGGATATTTTTTAGTGTGTAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCACGTTCATTCAATATATAAGATATATCACGATAATTGCGCATATAACTTAAGTAGT  >  USA300TCH1516_ALE/88693‑88970                                                                                                                                           |                                                                                                                                            aaCTTATTATTGTTAAGGAGGATATTTTTTAGTGTGTAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCag                                                                                                                                           >  2:499714‑M2/1‑139 (MQ=255) aaCTTATTATTGTTAAGGAGGATATTTTTTAGTGTGTAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCag                                                                                                                                           >  1:287073‑M2/1‑139 (MQ=255)       ttattGTTAAGGAGGATATTTTTTAGTGTGTAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCTCATCATTAACCGATACTCAGAGTCGt                                                                                                                                     >  2:609550/1‑141 (MQ=255)       ttattGATAAGGAGGATATTTTTTAGTGTGTAAATTAAAAAGAATTTTAGAAGAAAAACATTTATCAAAAAACTGTTCATTACCTTATCAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGcta                                                                                                                                     >  2:437891/1‑139 (MQ=255)        tattGTTAAGGAGGATATTTTTTAGTGTGTAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTa                                                                                                                                    <  1:575031/141‑1 (MQ=255)          ttGTTAAGGATGTTATTTTTTAGTGTTTAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTTACCGATACGCagaagcatatc                                                                                                                                  >  2:476795‑M2/1‑130 (MQ=255)          ttGTTAAGGAGGATATTTTTTAGTGTGTAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCagaagcatatc                                                                                                                                  >  1:6128‑M2/1‑130 (MQ=255)            gTTAAGGAGGATATTTTTTAGTGTGTAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCAt                                                                                                                                >  2:165853/1‑141 (MQ=255)            gTTAAGGAGGATATTTTTTAGTGTGTAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCAt                                                                                                                                >  2:26889/1‑141 (MQ=255)            gTTAAGGAGGATATTTTTTAGTGTGTAAATTAAAAAGAATTTTACAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGTGGCGAATCAt                                                                                                                                >  2:371106/1‑141 (MQ=255)                  gaggaTATTTTTTAGTGTGTAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTaa                                                                                                                          <  1:365935/141‑1 (MQ=255)                  atagaTATTTTATAGTGTTTAAATTAAAAAGAATTTAATAATAATAAAATTTAAAAAAAAACTGTTCATTACCTTATAAATTGAAATTATATAATTAAAAACAGAATCATTAACCGATACGCCGTGGCGTATCATaaaaaa                                                                                                                          <  2:354132/138‑5 (MQ=255)                   aggaTATTTTTTAGTGTGTAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTaaa                                                                                                                         <  1:55837/141‑1 (MQ=255)                      atatTTTTTAGTGTGTAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTACAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACt                                                                                                                      >  1:646535/1‑141 (MQ=255)                        atTTTTTAGTGTGTAAATTAAAAAGAATTTTAGAAGAACAAAATTTAACAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTaa                                                                                                                    <  2:259116/141‑1 (MQ=255)                         ttttttAGTGTGTAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGTTAAGTAGATGCGTATCATAAGTAAAACTaaa                                                                                                                   <  1:686269/141‑1 (MQ=255)                          tttttAGTGTGTAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTaaaa                                                                                                                  >  1:10628/1‑141 (MQ=255)                          tttttAGTGTGTAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTaaaa                                                                                                                  >  1:10615/1‑141 (MQ=255)                           ttttAGTGTGTAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTATTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTaaaaa                                                                                                                 >  2:423029/1‑141 (MQ=255)                              tAGTGTGTAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAAtt                                                                                                              <  2:459298/141‑1 (MQ=255)                              aagagtgtAAATTAAAAAGAATTTTAGAAGAATAACATTTAACAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGCAAAACTAAAAAAtt                                                                                                              <  2:474582/137‑1 (MQ=255)                               aGTGTGTAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTACAAAATTc                                                                                                             <  2:440694/141‑1 (MQ=255)                                gtgtgtAAATTAAAAAGAATTTTATAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCt                                                                                                            <  2:341640/141‑1 (MQ=255)                                  tttttttttAAAAAGAATTTTAGAAGAATAACATTTATCAGAAAACTCTTCATTACCTTATTAATTGAAATTATATTTTTAAAAATTGCATCATTAACCGATACGCAGAGGCGTATGATAAGTAAAACTAAAAAATTATGt                                                                                                          >  2:435008/8‑141 (MQ=255)                                  gtgtAAATTAAAAATAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACGGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGt                                                                                                          <  1:462379/141‑1 (MQ=255)                                  gtgtAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGt                                                                                                          <  1:462302/141‑1 (MQ=255)                                  gtgtAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGt                                                                                                          >  2:187671/1‑141 (MQ=255)                                   tgtAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTa                                                                                                         >  2:682168/1‑141 (MQ=255)                                   tgtAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTa                                                                                                         <  2:217597/141‑1 (MQ=255)                                      aaaTTAAAAAGAATTTTAGAATAATCACATCTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATGAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATga                                                                                                      <  1:204842/141‑1 (MQ=255)                                      aaaTTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACTGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAGACTAAAAAATTCTGTATga                                                                                                      <  1:417468/141‑1 (MQ=255)                                      aaaTTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATga                                                                                                      <  1:602408/141‑1 (MQ=255)                                            aaaaGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGat                                                                                                <  1:121268/141‑1 (MQ=255)                                              aaGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGataa                                                                                              <  1:654705/141‑1 (MQ=255)                                               aGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCagaagcatatcataaatgatgcggttttttcagccgcttcataaaggg                                                                                             >  2:413873‑M2/1‑93 (MQ=255)                                                gAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAATTCATGTTCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGataata                                                                                            <  1:163246/141‑1 (MQ=255)                                                aaaTTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGataata                                                                                            <  1:35700/140‑1 (MQ=255)                                                aaaTTTTAGAAGAATAACAATTATAAAAAAACTGTTCATTACCTTATTAATTGAAAGTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAAAAAAAAAAAAAAATTCTGCATGAGGAGataata                                                                                            <  2:643518/140‑1 (MQ=255)                                                 aaTTTTCGAAGAATAACATTTATCAAAAAACTCTTCAATACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGAtaataa                                                                                           <  2:553546/141‑1 (MQ=255)                                                 aaTTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGAtaataa                                                                                           <  2:451984/141‑1 (MQ=255)                                                 aaTTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTAACTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTAGCATAAGTAAAACTAAAAAATTCTGTATGAGGAGAtaataa                                                                                           <  2:683955/141‑1 (MQ=255)                                                  aTTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACTCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATaataat                                                                                          <  2:664271/141‑1 (MQ=255)                                                  aTTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATaataat                                                                                          <  2:400339/141‑1 (MQ=255)                                                     ttAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTg                                                                                       <  2:429983/141‑1 (MQ=255)                                                         aagaaTAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAgg                                                                                   >  2:585653/1‑141 (MQ=255)                                                           aaaTAACATTTATCAAAAAACCTTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGgt                                                                                 <  2:646833/140‑1 (MQ=255)                                                                 cATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTaaa                                                                           <  2:409849/141‑1 (MQ=255)                                                                  aTTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAAt                                                                          >  1:324352/1‑141 (MQ=255)                                                                  aTTAATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAAt                                                                          <  2:363241/141‑1 (MQ=255)                                                                   tttATCAAAAAACCTTTCATTACCTTATTAATTTAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAAtg                                                                         <  2:103476/141‑1 (MQ=255)                                                                        cAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGa                                                                    <  2:520448/141‑1 (MQ=255)                                                                         aaaaaaCTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGAc                                                                   <  1:266252/141‑1 (MQ=255)                                                                          aaaaaCTGTTCATTACCTTATTAATTGACATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACa                                                                  <  1:393066/141‑1 (MQ=255)                                                                           aaaaCTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCagaagcatatcataaatgatgcggttttttcagccgcttcataaagggattttgaatgtatcagaacatatgaggt                                                                 <  2:356490‑M2/141‑77 (MQ=255)                                                                           aaaaCTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACAt                                                                 >  1:528815/1‑141 (MQ=255)                                                                              acggatCATTACATGATGAAATGAAATTATAGAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTaa                                                              <  2:605102/136‑1 (MQ=255)                                                                               cTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTaaa                                                             <  2:687569/141‑1 (MQ=255)                                                                               cTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTaaa                                                             <  2:623763/141‑1 (MQ=255)                                                                                tGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAAt                                                            >  1:623240/1‑141 (MQ=255)                                                                                tGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAAt                                                            >  1:176704/1‑141 (MQ=255)                                                                                   tCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTTAATGGTGGACATTAAATCCa                                                         <  1:423241/141‑1 (MQ=255)                                                                                   tCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCa                                                         <  2:64338/141‑1 (MQ=255)                                                                                   tCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCa                                                         <  2:640496/141‑1 (MQ=255)                                                                                   tCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGATAATTGGTGGACATTAAATCCa                                                         <  2:493928/141‑1 (MQ=255)                                                                                   tCATGACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCa                                                         <  1:79841/141‑1 (MQ=255)                                                                                            tattaATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCACGttcattc                                                <  1:682168/141‑1 (MQ=255)                                                                                             attaATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCACGttcattca                                               >  1:688240/1‑141 (MQ=255)                                                                                                   tGAAATTATATAATTAAAAACCGTAAAGTTAACCTATACGCAGCGGATTGAAAAGAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCACGTTCATTCAatatat                                         <  1:677412/141‑1 (MQ=255)                                                                                                   tGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCACGTTCATTCAatatat                                         <  2:229010/141‑1 (MQ=255)                                                                                                   tGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCACGTTCATTCAatatat                                         <  1:533977/141‑1 (MQ=255)                                                                                                   tGAAAATATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCACGTTCATTCAatatat                                         <  2:19882/141‑1 (MQ=255)                                                                                                                 gaaaaaCCGCATCATTAACCGAAACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCACGTTCATTCAATATATAAGATATATCACGa                           <  2:398024/140‑1 (MQ=255)                                                                                                                    aaaCCGCATCATGAACCGATCCGCAGAGGCGTACCATAAGTAAAACTAAGAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCACGTTCATTCAATATATAAGATATATCACGATaa                        <  2:421769/141‑1 (MQ=255)                                                                                                                       ccgactcatTACCCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACACTAAATCCACGTTCATTCAATATATTAGATATATCACGATAATTg                     <  2:521422/136‑1 (MQ=255)                                                                                                                       ccGAATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCACGTTCATTCAATATATAAGATATATCACGATAATTg                     <  2:561866/141‑1 (MQ=255)                                                                                                                        cGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCACGTTCCTTCTATATATAAGATATATCACGATAATTgc                    <  1:165853/141‑1 (MQ=255)                                                                                                                        cGCATCATTAACCGATACGCAGAGGCCTATCATAAGTAAAACTACAAAATTCTGTATGAGGAGATAATAATTTGGAGAGTGTTAAATTGTGGACATTAAATCCACGTTCATTCAATATATAAGATATATCACGATAATTgc                    <  1:26889/141‑1 (MQ=255)                                                                                                                        cGCATAATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCACGTTCATTCAATATATAAGATATATCACGATAATTgc                    <  1:371106/141‑1 (MQ=255)                                                                                                                         gCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCACGTTCATTCAATATATATGATATATCACGATAATTgcg                   <  1:423029/141‑1 (MQ=255)                                                                                                                         ccatcacTAACCGATACGCAGAGGCGTATCATAAATAAAACTAAAAAATACTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCACGTTCATTCAATATATTAGATATATCACGATAATTgcg                   <  1:656253/140‑1 (MQ=255)                                                                                                                             catTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCACGTTCATTCAATATATAAGATATATCACGATAATTGCGCata               <  1:202068/141‑1 (MQ=255)                                                                                                                             catTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCACGTTCATTCAATATATAAGATATATCACGATAATTGCGCata               <  2:646535/141‑1 (MQ=255)                                                                                                                                tAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCACGTTCATTCAATATATAAGATATATCACGATAATTGCGCATATaa            <  2:338250/141‑1 (MQ=255)                                                                                                                                  aCCGATACGCAGAGGCGTCTCCTTCCAGGAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCACGTTCATTCAATATATAAGATATATCACGATAATTGCGCATATAACt          >  1:338250/1‑141 (MQ=255)                                                                                                                                    cGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCACGTTCATTCAATATATAAGATATATCACGATAATTGCGCATATAACTTa        >  1:145446/1‑141 (MQ=255)                                                                                                                                       tACGCAAAGGCGTATCATAAGTAAAACGAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCACGTTCATTCAATATATAAGATATATCACGATAATTGCGCATATAACTTAagt     <  2:87370/141‑1 (MQ=255)                                                                                                                                          gCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATTGTGGACATTAAATCCACGTTCATTCAATATATAAGATATACCACGCTAATTGCGCTTCTACATTAagtagt  >  1:66160/1‑141 (MQ=255)                                                                                                                                           |                                                                                                                                            AACTTATTATTGTTAAGGAGGATATTTTTTAGTGTGTAAATTAAAAAGAATTTTAGAAGAATAACATTTATCAAAAAACTGTTCATTACCTTATTAATTGAAATTATATAATTAAAAACCGCATCATTAACCGATACGCAGAGGCGTATCATAAGTAAAACTAAAAAATTCTGTATGAGGAGATAATAATTTGGAGGGTGTTAAATGGTGGACATTAAATCCACGTTCATTCAATATATAAGATATATCACGATAATTGCGCATATAACTTAAGTAGT  >  USA300TCH1516_ALE/88693‑88970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.