New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE = 2031791102 (1.180)4 (0.050) 3/286 7.7 4.0% intergenic (+257/+233) USA300HOU_RS10250/USA300HOU_RS10255 hypothetical protein/hypothetical protein
?USA300TCH1516_ALE = 2031829 96 (1.150)intergenic (+295/+195) USA300HOU_RS10250/USA300HOU_RS10255 hypothetical protein/hypothetical protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 103 total aligned reads displayed.

TTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATAAATATTCAGACTCAAATATAGCCACACGCCTAAACTACGGAGG  >  USA300TCH1516_ALE/2031683‑2031976
                                                                                                                                                  |                                                                                                                                                    
ttGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGTGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAg                                                                                                                                                    <  1:443547/149‑1 (MQ=255)
ttGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAg                                                                                                                                                    <  2:717079/149‑1 (MQ=255)
ttGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAg                                                                                                                                                    <  2:716878/149‑1 (MQ=255)
   tCTGTAGAAATTGGGTATCCACTTTCTCTTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTTTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACCAGCGCATTTTCGTTCAGTCa                                                                                                                                                 <  1:135834/149‑1 (MQ=255)
   tCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCa                                                                                                                                                 >  1:864735/1‑149 (MQ=255)
   tCTGTAGAAAGTGGGAATCCAATTTCTCTTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCCATTTCTCTTGGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCa                                                                                                                                                 <  1:444444/149‑1 (MQ=255)
    cTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCGTTTTCGTTCAGtaca                                                                                                                                                >  1:809240/1‑146 (MQ=255)
       tAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAac‑ta                                                                                                                                            >  2:623942/1‑149 (MQ=255)
          aaaTTGGGAATCCAATTTCTCTTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTg                                                                                                                                         <  2:381814/149‑1 (MQ=255)
          aaaTTGGGAATCCAATTTCTCTTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTg                                                                                                                                         >  1:505933/1‑149 (MQ=255)
          aaaTTGGGAATCCAATTTCTCTTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTg                                                                                                                                         <  1:838386/149‑1 (MQ=255)
          aaaTTGGGAATCCAATTTCTCTTTGTTGGGGCCCACACCCCAACTTGCATTGTCGGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTg                                                                                                                                         >  1:728361/1‑149 (MQ=255)
           aaTTGGGAATCGAATTTCTCTTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGc                                                                                                                                        <  2:443905/149‑1 (MQ=255)
           aaTTGGGAATCCAATTTCTTTTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTTTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGc                                                                                                                                        <  1:206035/149‑1 (MQ=255)
           aaTTGGGAATCCAATTTCTCTTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGc                                                                                                                                        <  1:525343/149‑1 (MQ=255)
            aTTGGGACTCCAATTTCTCTTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGcc                                                                                                                                       <  2:86847/149‑1 (MQ=255)
            aTTGGGACTCCAATTTCTCTTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATGTTCGTTCAGTCAAC‑TACTGcc                                                                                                                                       <  2:865570/149‑1 (MQ=255)
             ttGGGAATCCAATTTCTCTTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGTAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCa                                                                                                                                      <  2:4300/149‑1 (MQ=255)
             ttGGGAATCCAATTTCTCTTTGGTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCa                                                                                                                                      <  1:414835/149‑1 (MQ=255)
                ggAATCCAATTTCTCTTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGCATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCGAata                                                                                                                                   >  2:151835/1‑149 (MQ=255)
                ggAATCCAATTTCTCTTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAata                                                                                                                                   <  2:779106/149‑1 (MQ=255)
                    tCCAATTTCTCTTTGTTGGTGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTGCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAAc                                                                                                                               <  1:216656/149‑1 (MQ=255)
                       aaTTTCTTCTTGGTGGGGCCCACCCCCAAAGTTGCATTATCGGTAGAAATGTGGAATGCAATTTCTCTTTGTTGGTGCCCTTTACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTc                                                                                                                            <  2:277553/149‑1 (MQ=17)
                           tctcTTTGTTGGGGCCCACACCCCAACTTTGATTGTCTGTAGAAATTGGGAATCCAATTGCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTag                                                                                                                        <  2:847491/149‑1 (MQ=255)
                             ttcttgctgGGGCCCACCCCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAACCTCTTTATCCTGTTGCCCCTGACTAGAATTGAAAAAAGCTTGTTCCAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTagag                                                                                                                      <  1:442312/146‑1 (MQ=17)
                             tctTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTagag                                                                                                                      <  2:898584/149‑1 (MQ=255)
                             tctTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAACATAACTTCGTagcg                                                                                                                      <  2:450425/149‑3 (MQ=255)
                             tcggtgtcgGGGCCCACACCCCAACTCGCATTGTCGGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGGACTAGAATTGTAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTagag                                                                                                                      <  1:66215/145‑1 (MQ=17)
                              ctTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGc                                                                                                                     >  1:730107/1‑149 (MQ=255)
                                 ttttgCGGCCCACACCCCAACTTGCATTGTGTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTACTATTGAAAAATGCGTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATa                                                                                                                  <  1:404496/147‑1 (MQ=18)
                                 tgttgGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATa                                                                                                                  <  1:387286/149‑1 (MQ=255)
                                  gttgGGGCCCACCCCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAg                                                                                                                 <  1:665645/149‑1 (MQ=255)
                                  ggtgGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATGTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGCGCATAg                                                                                                                 >  2:35029/3‑149 (MQ=255)
                                          ccACACACCAACCTGGCTTGTCTCCAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGAAAAAAAAAAAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGa                                                                                                         <  2:555276/149‑1 (MQ=17)
                                           cacaCCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAACGCTAAAAACGTGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGAt                                                                                                        <  1:875347/149‑1 (MQ=18)
                                           cacaCCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGAt                                                                                                        <  2:163409/149‑1 (MQ=255)
                                           cacaCCCCAACTTGCATTGGCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGAt                                                                                                        <  1:239453/149‑1 (MQ=255)
                                              acCCCAACTTGCACTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACGGCCAATATAACTTCGTAGAGCATAGAACATTGATTTa                                                                                                     <  1:757410/149‑1 (MQ=255)
                                                        gCATTGTCTGTAGACATTGGGAAGCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TCCTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGcc                                                                                           <  2:717211/149‑1 (MQ=255)
                                                         cATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCct                                                                                          <  1:623942/149‑1 (MQ=255)
                                                         cATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATGGCAACAAGCTTGTTACAAGCGCATAATCGTTAAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCAGAGAACATTGATGTATGTCTCCGccc                                                                                          >  2:152873/1‑148 (MQ=17)
                                                          aTTGTCTGTAGAAACTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGGTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCctc                                                                                         <  1:876637/149‑1 (MQ=255)
                                                             gTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCttt                                                                                      <  2:300967/149‑1 (MQ=255)
                                                              tCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCtttt                                                                                     >  2:725739/1‑149 (MQ=255)
                                                              tCTGTAGAAATTGGGAATCCAATTTCTCTTTGCCGCCAACCCTGACTAGAATTCCTAATAGCATGTTACAAGCGCCGTTTCTGTCAGGCAAC‑TACTGCCAATATAACTTCGTGGAGCATAGAACATTGATTTATGTCCCCGCCTCtttt                                                                                     >  2:35906/1‑149 (MQ=17)
                                                               cTGGAGACATTGGCAATCCAATTTCTCTTCGTTGTGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCttttt                                                                                    <  1:504160/149‑1 (MQ=17)
                                                                tGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTa                                                                                   <  1:824733/149‑1 (MQ=255)
                                                                tGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAACCTTCATCCACTCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTa                                                                                   <  2:330744/149‑1 (MQ=17)
                                                                 gTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTAt                                                                                  <  1:794403/149‑1 (MQ=255)
                                                                    gAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGt                                                                               <  1:45907/149‑1 (MQ=255)
                                                                    gAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGt                                                                               <  2:810455/149‑1 (MQ=255)
                                                                    gAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGt                                                                               <  1:201666/149‑1 (MQ=255)
                                                                     aaaTTGGGAATCCAATTTCTCTTTGTTGGGGCCACTGACTCAAATTGAAAAAAGATAGTTAGAATCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGta                                                                              <  1:213731/149‑1 (MQ=17)
                                                                         tGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATTTCCCCGCCTCTTTTTATCGtatata                                                                          >  1:376228/1‑149 (MQ=255)
                                                                         tGGGAATCCAATTCCTCTTCGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTCCAAGCGCATTTTCGTTCAGCCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCACCGCCACTCTTTATCGtatata                                                                          >  2:3539/1‑149 (MQ=17)
                                                                          gggAATCCAATTTTTCTTTGTTAGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAg                                                                         <  2:404917/149‑1 (MQ=255)
                                                                          gggAATCCAATTTCTATTTGTTGGGGGCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TAATGCCACTATAACTGCGTAGAGCATAGAACATTGATTTTTGTCCCCGCCTCTTTTTATCGTATATAg                                                                         <  1:565516/149‑1 (MQ=17)
                                                                          ggaaaTCTAAGTTCTCTTTGTTGTGGCCCTTGACAAAAATTGAAACAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAg                                                                         <  2:451186/146‑1 (MQ=17)
                                                                            gAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCa                                                                       <  2:442832/149‑1 (MQ=255)
                                                                             aaTCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCAt                                                                      >  2:390879/1‑149 (MQ=255)
                                                                             aaTCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTAAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATGGATTTATGTCCCCGCCTATTTTTACCGTAGATAgact                                                                      >  2:702483/1‑146 (MQ=14)
                                                                                 cAATTTCTCTTTGTTGGGCCCCCTGACTAGAATTGAAAAACGCCTGTTCAAAGAGCATATTAAATAAGTAAAA‑AACTGTGAATTTAATTTCGTAGATCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTcc                                                                  <  2:331852/149‑1 (MQ=17)
                                                                                     ttCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCttt                                                              >  1:114869/1‑149 (MQ=255)
                                                                                       ctctTTGTTGGGGCCCCTGCATAGCATTGAAACGAGGTTGTCTCTGGCGCATTTTCGTTCAGTCAAC‑TATTTCCAATAGAGCTTCGTATAGCATATAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCttttt                                                            <  2:539613/149‑1 (MQ=18)
                                                                                         ctTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTaa                                                          >  2:102849/1‑149 (MQ=255)
                                                                                              ttgGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTa                                                     >  2:266086/1‑149 (MQ=255)
                                                                                              ttgGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGCCCCCGCCTCTTTTTACCGTATATAGCATGTCCCTTTTTAAAAttt                                                     <  1:735445/149‑2 (MQ=255)
                                                                                               tgGGGCCCCTGACTAGAATTGAAAACAGCTTGTTACAAGCGCAGTTTCGTTCAGTCAAC‑TAATGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCCTGTCCCTTTTTAACATTaa                                                    >  2:83155/1‑148 (MQ=17)
                                                                                               tgGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACACGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTGACATTaa                                                    >  2:385629/1‑148 (MQ=255)
                                                                                                 ggtccccTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACaa                                                  <  2:434344/146‑1 (MQ=255)
                                                                                                 ggggCCCTGACTAGAATTGAAAAAAGCTTGTTTCTAGCGTATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACaa                                                  <  2:462106/149‑1 (MQ=255)
                                                                                                 gggCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TGCTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACaa                                                  <  2:778041/149‑1 (MQ=255)
                                                                                                 gggCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATGGCATGTCCCTTTTTAAAATTACaa                                                  >  2:892353/1‑149 (MQ=255)
                                                                                                 gggCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAAAATACCTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACaa                                                  >  2:148942/1‑149 (MQ=255)
                                                                                                    ccccTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCa                                               <  1:393207/149‑1 (MQ=255)
                                                                                                     tccTGAATAGAAATGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAT‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCAt                                              <  2:263150/148‑1 (MQ=255)
                                                                                                        tGACTAGAATTGAAAAAATCTGGTTACAGGCGCCTTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATaaa                                           <  1:233216/149‑1 (MQ=255)
                                                                                                        tGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATaaa                                           <  1:782941/149‑1 (MQ=255)
                                                                                                        tGACTAGAAGTTACAAAAGCGTGTTACAGGCGCATTTTCGTTCCGTCAAC‑TCCTGCCAATATAACTTCGTAGAGCATAGAACATTGCTTTCTGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTTAAATTACAAGCATaaa                                           <  2:307656/149‑1 (MQ=18)
                                                                                                         gACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATCGCATGTCCCTTTTTAAAATTACAAGCATAAat                                          >  2:651156/1‑149 (MQ=255)
                                                                                                           cTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATAAatat                                        <  2:682772/149‑1 (MQ=255)
                                                                                                           cTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATAAatat                                        >  1:848587/1‑149 (MQ=255)
                                                                                                           cTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATAAatat                                        <  1:331873/149‑1 (MQ=255)
                                                                                                           cTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTAAG‑CATCTTGTTACCAATCTGATGTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATAAatat                                        >  1:445950/1‑149 (MQ=21)
                                                                                                            tcgAATTGAAAAAAGCTTGTCACCAGCGCATTTTCGTTCAGTCAAC‑TTCTGCTCATTTAACTTCGTAGGGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGTATGTCCCTTTTTAAAATTACAAGCATAAATAtt                                       <  2:747117/147‑1 (MQ=21)
                                                                                                            tAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATAAATAtt                                       <  1:97983/149‑1 (MQ=255)
                                                                                                                aTTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATAAATATTCAGa                                   <  2:152311/149‑1 (MQ=255)
                                                                                                                aTTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCACAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATAAATATTCAGa                                   <  2:479543/149‑1 (MQ=255)
                                                                                                                   gAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATAAATATTCAGACTc                                <  1:616876/149‑1 (MQ=255)
                                                                                                                              tttACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATCAATATTCAGACTCAAATATAGCca                     <  1:463026/148‑1 (MQ=255)
                                                                                                                              tttACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATAAATATTCAGACTCAAATATAGCca                     <  2:161001/148‑1 (MQ=255)
                                                                                                                                tACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATAAATATTCAGACTCAAATATAGCcaca                   >  1:307350/1‑149 (MQ=255)
                                                                                                                                  cacgcgcATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATAAATATTCAGACTCAAATATAGCCACACg                 <  2:815294/146‑1 (MQ=255)
                                                                                                                                  cAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATAAATATTCAGACTCAAATATAGCCacacc                 >  2:827769/1‑148 (MQ=255)
                                                                                                                                   aatcgcATTTTCGTTCAGTCAAC‑TACTGACAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATAAATATTCAGACTCAAATATAGCCACACGc                <  2:628865/146‑1 (MQ=255)
                                                                                                                                    atctcATTTTCGTTCATCCAAC‑TGCTGCCAATATAAGTTCGTAGAGCATAGAACGTTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATAAATATTCAGACTCAAATATAGCCACACGcc               <  1:520414/145‑1 (MQ=255)
                                                                                                                                    aGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATAACTATTCAGACTCAAATATAGCCACACGcc               <  1:563291/149‑1 (MQ=255)
                                                                                                                                       gctttttCGTTCAGTCAAC‑TACTGCCAATCTAACTTCGTAGAGCATAGAACATTGCTTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATAAATATTCAGACTCAAATATAGCCACACGCCTaa            <  2:69697/146‑1 (MQ=255)
                                                                                                                                            ttCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATCTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATAAATATTCAGACTCAAATATAGCCACACGCCTAAACTAc       <  2:541698/149‑1 (MQ=255)
                                                                                                                                                 tCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATAAATATTCTTACTCAAAGATAGCCACACGCCTAAACTACGGAgg  <  2:199699/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCACACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGTCAAC‑TACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCCGCCTCTTTTTATCGTATATAGCATGTCCCTTTTTAAAATTACAAGCATAAATATTCAGACTCAAATATAGCCACACGCCTAAACTACGGAGG  >  USA300TCH1516_ALE/2031683‑2031976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.