New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	= 800928	93 (0.910)	5 (0.050)	5/282	7.6	5.2%	intergenic (+62/‑289)	hisC_1/USA300HOU_RS03915	Histidinol‑phosphate aminotransferase/Putative 5'(3')‑deoxyribonucleotidase
	?	USA300TCH1516_ALE	801948 =	92 (0.940)					intergenic (+189/+435)	USA300HOU_RS03915/USA300HOU_RS03920	Putative 5'(3')‑deoxyribonucleotidase/Putative lipid kinase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTTCCAACTGGTGTTAGAATTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑CAACTACTGCCAATATAACTTTGTAGAGCATTGAACA  >  USA300TCH1516_ALE/800780‑801072                                                                                                                                                     |                                                                                                                                                  tttCCAACTGGTGTTAGAATTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACGCCAAc                                                                                                                                                   >  1:1071803/1‑149 (MQ=255)    ccAACTGGTGTTAGAATTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACGTTCGCAACATTTCTTTCGTCGTCCCACCCCAACTTg                                                                                                                                                <  2:1020467/149‑1 (MQ=255)     cAACTGGTGTTAGAATTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCTAACTTGc                                                                                                                                               <  2:542314/149‑1 (MQ=255)      aaCTGGTGTTAGAATTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGct                                                                                                                                              >  2:681540/1‑148 (MQ=255)      aaCTGGTGTTAGAATTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCa                                                                                                                                              <  1:467146/149‑1 (MQ=255)            tgtTAGAATTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTAGTCCCACCCCAACTCGCACTGTCt                                                                                                                                        >  2:345751/1‑149 (MQ=255)               tAGAATTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTAGTCGTCCCACCCCAACTTGCATtgtatgta                                                                                                                                     >  1:1000365/1‑149 (MQ=255)               tAGAAGTACAATTGGTTCTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTa                                                                                                                                     <  1:962921/149‑1 (MQ=255)                 gAATTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGa                                                                                                                                   >  1:344627/1‑149 (MQ=255)                 gAATTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTAGTCCCACCCCAACTTGCATTGTCTGTAGa                                                                                                                                   >  1:424860/1‑149 (MQ=255)                   aTTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGaaa                                                                                                                                 <  1:492401/149‑1 (MQ=255)                   aTTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTGTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGaaa                                                                                                                                 >  1:854836/1‑149 (MQ=255)                      aCAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTg                                                                                                                              >  1:184204/1‑149 (MQ=255)                       cAATTGGTTTTAAAGAACAGAATGATAAAATGTTAGAAGTTTCAGCAACCTTTGAATTCGAAGATAAAGAAACGAGGGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGGCTGTAGAAATTgg                                                                                                                             >  2:1068559/1‑149 (MQ=18)                       cAATTGGTTTTAAAGAACAAAATGATAAAATGTTATAATTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTgg                                                                                                                             >  2:101471/1‑149 (MQ=255)                       cAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTgg                                                                                                                             >  2:446545/1‑149 (MQ=255)                       cAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTAGCAAAATTTATTTCGTCGTCCCACACCAACTTGCATGTTCTGTAGAAATTgg                                                                                                                             >  2:590711/1‑149 (MQ=255)                            ggTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATc                                                                                                                        >  2:88232/1‑149 (MQ=255)                            ggTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATc                                                                                                                        <  2:1083841/149‑1 (MQ=255)                              ttttAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCa                                                                                                                      <  2:400069/149‑1 (MQ=255)                               tttAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCaa                                                                                                                     >  1:844629/1‑149 (MQ=255)                                ttAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCCAAATTTATTTCGTCGTACCACCCCAACTTGCCATGTCTGTAGAAATTAGGaagaacat                                                                                                                    >  1:80627/1‑143 (MQ=25)                                ttAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAAt                                                                                                                    >  1:645340/1‑149 (MQ=255)                                   aaGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTtc                                                                                                                 >  2:746284/1‑149 (MQ=255)                                      aaCAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTctct                                                                                                              >  2:157708/1‑149 (MQ=255)                                      aaCAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTctct                                                                                                              >  2:925746/1‑149 (MQ=255)                                               aTAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTgggg                                                                                                     <  1:746284/149‑1 (MQ=255)                                               aTAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTgggg                                                                                                     >  2:547454/1‑149 (MQ=255)                                                       ttAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCccgccg                                                                                             <  2:208663/149‑1 (MQ=255)                                                          gAAGTTTTATCAAACTTTAAATACGAATGGTAAGTGGGGAGTGAGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTATAGAAATTGTGACTCCAATTTCCCTTTTGTGTGGCCCCGCCGGCa                                                                                          >  2:297552/1‑149 (MQ=18)                                                           aaGATTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATCTCTCTTTGTTGGCGCCCCGCCGGCaa                                                                                         >  2:739581/1‑149 (MQ=255)                                                                ttATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTg                                                                                    >  1:30667/1‑149 (MQ=255)                                                                ttATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTg                                                                                    <  1:234073/149‑1 (MQ=255)                                                                 tATCAAACTTTAAATGCGAATAGTAAGTGGGGAGTGGGACAGGAATGTTACTTTCGCAAAATTTATTTCGTCGTCCCCCCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGa                                                                                   <  2:319157/149‑1 (MQ=255)                                                                  aTCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGAc                                                                                  >  2:698584/1‑149 (MQ=255)                                                                   tCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACt                                                                                 <  1:1045140/149‑1 (MQ=255)                                                                   tCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACt                                                                                 <  1:679601/149‑1 (MQ=255)                                                                    cAAACTTTAAATACGAATAGTAAGTGGGGGGAGTGGCAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCCAGGTTGACTa                                                                                >  2:1073208/1‑149 (MQ=17)                                                                    cAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTa                                                                                >  2:387689/1‑149 (MQ=255)                                                                      aaCTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGa                                                                              <  2:473866/149‑1 (MQ=255)                                                                               tACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATGCAAGTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGaaaaa                                                                     >  1:585544/1‑149 (MQ=255)                                                                                 cGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGGCTAGCATTGAAAACAg                                                                   >  1:944656/1‑149 (MQ=255)                                                                                 cGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAg                                                                   >  1:567383/1‑149 (MQ=255)                                                                                 cGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAg                                                                   >  1:1062059/1‑149 (MQ=255)                                                                                 cGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCAACCCCAACTTGCATGGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAg                                                                   >  1:128967/1‑149 (MQ=255)                                                                                  gAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCAAACTTGCGTTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGc                                                                  >  1:704635/1‑149 (MQ=255)                                                                                   attcgTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACACCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCt                                                                 >  1:748961/5‑149 (MQ=255)                                                                                    aTAGTAAGGGGGGGGTGGGACAGAAATTCTACTTCCGCAATGTTTATTTCGGCGTCGTACCCCGATGTGCTTTGTCTGTAGAAATTGGGATTCCAATTTCTCTTTGTTGGGGCCCCGCCGGGAAGGTTGACTAGAATTGAAAGAAGCtt                                                                <  2:182289/149‑1 (MQ=14)                                                                                      aGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCTCGACTTGCAGTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGt                                                              <  2:415481/149‑1 (MQ=255)                                                                                       gTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGtt                                                             <  1:1070220/149‑1 (MQ=255)                                                                                       gTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGtt                                                             >  2:27328/1‑149 (MQ=255)                                                                                        tAAGTGGGGAGTGGGGCAGAAATGATACTTTCGCAAGAGTTATTTAGTGATCACAGGGTAACGCCACTCGTCTGTAGAAATTGGAAATACAATTTCTCTTTGTTGCGGCCCCGCCGGCCAGGGTTACTAAAATTTCAAACAACATGTTa                                                            >  2:733146/1‑149 (MQ=255)                                                                                        tAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGttc                                                            >  1:1075648/1‑148 (MQ=255)                                                                                        tAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTa                                                            >  1:962771/1‑149 (MQ=255)                                                                                        tAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTa                                                            >  1:1072128/1‑149 (MQ=255)                                                                                        tAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTGGTTa                                                            >  2:656065/1‑149 (MQ=255)                                                                                         aaGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTAc                                                           >  2:433429/1‑149 (MQ=255)                                                                                           gTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACaa                                                         >  1:791771/1‑149 (MQ=255)                                                                                           gTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACaa                                                         >  1:967490/1‑149 (MQ=255)                                                                                            tGGGGAGTGGGACTGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAg                                                        <  1:419529/149‑1 (MQ=255)                                                                                            tGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTGGTTACAAg                                                        >  2:244458/1‑149 (MQ=255)                                                                                              tagattGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAgcg                                                      <  2:1109922/144‑1 (MQ=255)                                                                                                gAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCa                                                    >  1:186601/1‑149 (MQ=40)                                                                                                   ggggACAGAAATGATACTGTCGCAAAATTTAGTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGATTAGAATTGAAAAAAGCTTGTTACAAGCGCAttt                                                 <  1:88818/148‑1 (MQ=255)                                                                                                    gggcaaGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCCCCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTCGGCTAGAATTGAAAAAAGCTTGTTACAAGCGCAtttt                                                <  2:1071803/144‑1 (MQ=255)                                                                                                    gggACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCAtttt                                                >  1:718723/1‑149 (MQ=255)                                                                                                     ggACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTc                                               <  2:690925/149‑1 (MQ=40)                                                                                                     ggACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTc                                               <  1:275705/149‑1 (MQ=40)                                                                                                      gACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCTGCAAGTTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCg                                              <  2:873536/149‑1 (MQ=255)                                                                                                        cAGAAATGATACTTTCGCAAAATTTAGTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGtt                                            <  2:473368/149‑1 (MQ=255)                                                                                                          gAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAGGAGCATTTTCGTTCa                                          >  2:804389/1‑149 (MQ=255)                                                                                                              tGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑Ca                                     >  1:86884/1‑149 (MQ=255)                                                                                                              tGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTAAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑Ca                                     >  1:86851/1‑149 (MQ=255)                                                                                                                    tttCGCAAAACTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTGGCAATTGGGAAACCAATTTCTCGTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGACTTGAGAAACGCTTGTCACAAGCGCATTTTCGTT‑AGTGCAactacg                               >  2:274523/1‑148 (MQ=11)                                                                                                                     ttCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑CAACTACTg                              <  2:427168/149‑1 (MQ=35)                                                                                                                      tCGCAAAATTTATTTCGTCGTCCTGCCGCAACCTGCATTGTCTGTAGAAGTTGTGAATCCAATTTCTGGTGGTGGTGTCCCAGCCGGCAAGTTTTACTAGAATGGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑CAACTACTGc                             <  1:1096194/149‑1 (MQ=11)                                                                                                                       tgcatcgtttatttCGTCGTCCCACCCCAACTTCCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAACAGCTTGTTACAGGCGCAGTTTCGTTCAGT‑CAACTACTGcc                            <  2:809911/142‑1 (MQ=11)                                                                                                                         cAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑CAACTACTGCCaa                          <  1:925746/149‑1 (MQ=34)                                                                                                                         cAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑CAACTACTGCCaa                          <  1:157708/149‑1 (MQ=34)                                                                                                                         cAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAACCCAATCTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑CAACTACTGCCaa                          <  1:88232/149‑1 (MQ=18)                                                                                                                          caaaTTGATTTCGTCGTCCCACCCCAACTTGCATTGTCTGGAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGATTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑CAACTACTGCCAat                         <  1:339327/148‑1 (MQ=255)                                                                                                                           aaaaTTATTTCGGCGTCCCACCCCAACTTGCATTTTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑CAACTACTGCCAata                        <  2:385310/149‑1 (MQ=255)                                                                                                                           aaaTTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑CAACTACTGCCAata                        <  2:152135/149‑1 (MQ=34)                                                                                                                                 ttttCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑CAACTACTGCCAATATAACtt                  <  1:681540/148‑1 (MQ=25)                                                                                                                                  tttCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑CAACTACTGCCAATATAACttt                 <  2:854836/149‑1 (MQ=35)                                                                                                                                   ttCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑CAACTACTGCCAATATAACTTTg                <  1:77547/149‑1 (MQ=35)                                                                                                                                     cgtcgtCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑CAACTACTGCCAATATAACTTTGTa              >  1:349294/1‑149 (MQ=35)                                                                                                                                      gtcgtcCCCCCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑CAACTACTGCCAATATAACTTTGTag             <  1:519094/149‑1 (MQ=25)                                                                                                                                       tcgtcCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑CAACTACTGCCAATATAACTTTGTaga            <  2:669332/149‑1 (MQ=35)                                                                                                                                       tcgtcCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑CAACTACTGCCAATATAACTTTGTaga            >  1:918869/1‑149 (MQ=35)                                                                                                                                          taccACCCCAACTTGCATTGTCTGTAAAAATTGGGAGTCCAATTGCTGGTTGTTGGGGCCCCGGCGGCAAATGTGACTAGAATTGAAAAGGGCTTTTTATAAGCGCATTTTCGTTCAGT‑CAACTACTGCCAATATAACTTTGTAGAGCa         <  1:230089/147‑1 (MQ=11)                                                                                                                                              aCCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑CAACTACTGCCAATATAACTTTGTAGAGCATTGa     <  1:27328/149‑1 (MQ=38)                                                                                                                                                 ccAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑CAACTACTGCCAATATAACTTTGTAGAGCATTGAACa  <  2:407975/149‑1 (MQ=38)                                                                                                                                                 ccAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑CAACTACTGCCAATATAACTTTGTAGAGCATTGAACa  <  1:904771/149‑1 (MQ=38)                                                                                                                                                     |                                                                                                                                                  TTTCCAACTGGTGTTAGAATTACAATTGGTTTTAAAGAACAAAATGATAAAATGTTAGAAGTTTTATCAAACTTTAAATACGAATAGTAAGTGGGGAGTGGGACAGAAATGATACTTTCGCAAAATTTATTTCGTCGTCCCACCCCAACTTGCATTGTCTGTAGAAATTGGGAATCCAATTTCTCTTTGTTGGGGCCCCGCCGGCAAGGTTGACTAGAATTGAAAAAAGCTTGTTACAAGCGCATTTTCGTTCAGT‑CAACTACTGCCAATATAACTTTGTAGAGCATTGAACA  >  USA300TCH1516_ALE/800780‑801072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.