New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	= 800928	93 (0.910)	5 (0.050)	5/282	7.6	5.2%	intergenic (+62/‑289)	hisC_1/USA300HOU_RS03915	Histidinol‑phosphate aminotransferase/Putative 5'(3')‑deoxyribonucleotidase
	?	USA300TCH1516_ALE	801948 =	92 (0.940)					intergenic (+189/+435)	USA300HOU_RS03915/USA300HOU_RS03920	Putative 5'(3')‑deoxyribonucleotidase/Putative lipid kinase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ATAGGCTATAAAGTAATTGTGACTGAGATGAACTTTTATGTCTTAGACACTACAACACTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCTACATAATCAATCATTAGTCGTTCTTTATCATTTCCGTCCC  >  USA300TCH1516_ALE/801800‑802096                                                                                                                                                     |                                                                                                                                                     aTAGGCTATAAAGTAATTGTGACTGAGATAAACTTTTATGTCTTAGACACTACAACACTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATGGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACa                                                                                                                                                      <  2:495508/149‑1 (MQ=255)   aGGCTATAAAGTAATTGTGACTGAGATGAACTTTTATGTCTTAGACACTACAACACTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATa                                                                                                                                                    <  1:584633/149‑1 (MQ=255)     gCTATAAAGTAATTGTGACTGAGATGAACTTTTATGTCTTAGACACTACAACACTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGGCCCAACATaaa                                                                                                                                                  >  2:978124/1‑149 (MQ=255)       tataAAGTAATTGTGACTGAGATGAACTTTTATGTCTTAGACACTACAACACTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGa                                                                                                                                                <  2:132524/149‑1 (MQ=255)        ataAAGTAATTGTGACTGAGATGAACTTTTATGTCTTAGACACTACAACACTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGaa                                                                                                                                               >  1:747379/1‑149 (MQ=255)          aaaGTAATTGTGACTGAGATGAACTTTTATGTCTTAGACACTACAACACTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGCGGGCCCCAACATAAAGAAAt                                                                                                                                             >  2:605395/1‑149 (MQ=255)             gTAATTGTGACTGAGATGAACTTTTATGTCTTAGACACTACAACACTATATTCGCAGTCGTTGCCTGCGGGGACGCCACATAAAGCAATTGGAACGCGCATTGCTACAGTCAACTCAAGTTAGGGCTGGCCCCAACAGAAAGAAATACt                                                                                                                                          >  2:648652/1‑149 (MQ=255)                aTTGTGACTGAGATGAACTTTTATGTCTTAGACACTACAACACTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGATAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACtttt                                                                                                                                       <  1:1100531/149‑1 (MQ=255)                 ttGTGACTGAGATGAACTTTTATGTCTTAGACACTACAACACTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACttttt                                                                                                                                      <  2:154439/149‑1 (MQ=255)                         gagaTGAACTTTTATGTCTTAGACACTACAACACTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGaa                                                                                                                              >  2:1094234/1‑149 (MQ=255)                          ataTGAACTTTTATGTCTTAGAGACTACAACACTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGaaa                                                                                                                             <  2:489972/147‑1 (MQ=255)                          agaTGAACTTTTATGTCTTAGACACTACAACACTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGaaa                                                                                                                             <  2:909845/149‑1 (MQ=255)                             tGAACTTCTATGTCTTAGCCACTACAACACTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTa                                                                                                                          <  2:645425/149‑1 (MQ=255)                               aaCTTTTATGTCTTAGACACTACAACACTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGt                                                                                                                        >  2:384858/1‑149 (MQ=255)                                aCTTTTATGTCTTAGACACTACAGCACTATATTGGCAGTAGTTGACTGCGGGGCCCCATCATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATACAGAAATACTTTTACTTTAGAAATTAGTa                                                                                                                       <  1:703283/149‑1 (MQ=255)                                aCTTTTATGTCTTAGACACTACAACACTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTa                                                                                                                       <  1:234183/149‑1 (MQ=255)                                    ttATGTCTTAGACACTACAACACTATATTGGGAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTc                                                                                                                   <  2:505246/149‑1 (MQ=255)                                       tGTCTTAGACACTACACCACTCTATTGGCAGTAGTGGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTa                                                                                                                <  1:174735/149‑1 (MQ=255)                                        gTCTTAGACACTACAACACTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTGGTATTTCtttt                                                                                                               >  2:689710/1‑147 (MQ=255)                                          cTTAGACACTACAACACTATATTGGCAGTAGTTGACTGCGGGGCTCCAACATAGAGAACTTGGAACGCCAATTTCGAAAGACAATGCAAGATGGGGAGGGCCCCAACATAAAGAAATACTTTTTCTGTAGAAATTAGTATTTCTTATGc                                                                                                             >  1:771734/1‑149 (MQ=21)                                          cTTAGACACTACAACACTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTAGGc                                                                                                             >  1:692188/1‑149 (MQ=255)                                              gACACTACAACACTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGa                                                                                                         >  1:453898/1‑149 (MQ=255)                                                cacTACAACACTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTGGAAATTAGTATTTCTTATGCATGAgt                                                                                                       >  2:745256/1‑149 (MQ=255)                                                 acTACAACACTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTGCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAgtg                                                                                                      <  1:391210/149‑1 (MQ=255)                                                   cacaacaCTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACTCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTa                                                                                                    <  2:1081098/148‑1 (MQ=255)                                                      aacaCTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACt                                                                                                 <  1:865859/149‑1 (MQ=255)                                                      aacaCTATATTGGCAGTAGTTGACTGCGGGGCCCCAACAGAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACt                                                                                                 <  2:643358/149‑1 (MQ=255)                                                          cTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGCGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAACGAAATACTTTTTCTTTAGAAATTAGTATTTCGTATGCATGCGTGTAACTCATg                                                                                             >  1:982391/1‑149 (MQ=255)                                                          cTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATg                                                                                             <  2:888127/149‑1 (MQ=255)                                                            atatTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCa                                                                                           <  1:384858/149‑1 (MQ=255)                                                             tatTGGGAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCAt                                                                                          <  2:459367/149‑1 (MQ=255)                                                               ttGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCTAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTc                                                                                        <  1:703826/149‑1 (MQ=255)                                                               ttGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTc                                                                                        >  2:92112/1‑149 (MQ=255)                                                                tGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCa                                                                                       <  1:733392/149‑1 (MQ=255)                                                                 ggCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATACAGAAACACTTTTTATATAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCat                                                                                      <  2:733646/149‑1 (MQ=255)                                                                  ggaatagtTGACTCCCGGGTCCAAGCATAGGGTAATTGGCCCGCCAATTTTTATAGACAATGCACGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCata                                                                                     <  2:455122/145‑1 (MQ=17)                                                                  gCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCata                                                                                     >  2:795320/1‑149 (MQ=255)                                                                  gCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCata                                                                                     >  2:200848/1‑149 (MQ=255)                                                                  gCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTAGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGAATTTCTTATTCATGAGTGTAACTCATACATTCata                                                                                     >  2:253138/1‑149 (MQ=255)                                                                  gCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTAAAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATCCata                                                                                     >  2:187392/1‑149 (MQ=255)                                                                   cAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGCACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCatat                                                                                    <  2:52691/149‑1 (MQ=255)                                                                   cAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGGGTAACTAATGCATTCatat                                                                                    >  2:901546/1‑149 (MQ=255)                                                                     gtagtTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATAttt                                                                                  <  1:721147/149‑1 (MQ=255)                                                                        gtTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAACGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTATTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCCTGCATTCATATTTTTa                                                                               <  2:821853/149‑1 (MQ=255)                                                                         tttaCTGCTGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATCCTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTaa                                                                              <  2:561569/146‑1 (MQ=255)                                                                          tGACTTCGGGGCCCCAACATAGAGAAATTGGACCTCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAg                                                                             <  2:30642/149‑1 (MQ=255)                                                                          tGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCCTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAg                                                                             >  2:1002771/1‑149 (MQ=255)                                                                          tGACTGCGGGGCCCAAAAATAGATAAACTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAg                                                                             <  2:221063/149‑1 (MQ=255)                                                                            aCTGCGGGGCCCCAACGTAGAGAAATTGGAACGCCAATTTCTACCGTCAATGCAAGTTGGGGTGGGCGCCAAAATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTATGTa                                                                           <  2:395341/149‑1 (MQ=17)                                                                            aCTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAGTTAGTATTTCTTCTGCATGAGTGTAACTCATGCATTCAGATTTTTAAGtc                                                                           >  1:700267/1‑148 (MQ=255)                                                                             ccgCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTCATGCAGGAGTTGAACTCATGCATTCATATTTTTAAGTac                                                                          <  1:1102241/147‑1 (MQ=255)                                                                             cTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGATATTCGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTac                                                                          <  2:733300/149‑1 (MQ=255)                                                                             cTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTac                                                                          <  2:643457/149‑1 (MQ=255)                                                                             cTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTac                                                                          >  1:437038/1‑149 (MQ=255)                                                                             cTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTac                                                                          <  1:1094234/149‑1 (MQ=255)                                                                               gCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACGATGAAAGGTGGAGGGGGCCCCCCCATACAGAAATCCTTTTTCTTTAGAAATTGGTATTTCTTATGCATGAACGTAACGAATGCAGTAGTATTTTTAAGtcaca                                                                        >  2:184935/1‑145 (MQ=17)                                                                                cGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTCGAAATTAATATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTCAGTAcaca                                                                       >  2:328184/1‑149 (MQ=255)                                                                                  gggCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACAtt                                                                     >  1:437989/1‑149 (MQ=255)                                                                                  gggCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCAAATTTTTAAGTACACAtt                                                                     >  1:36492/1‑149 (MQ=255)                                                                                     ccccAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGc                                                                  <  1:912356/149‑1 (MQ=255)                                                                                       ccAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCtg                                                                <  2:747379/149‑1 (MQ=255)                                                                                           cATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCCTGCACGGATATTTTTAAGTACACATTAGCTGTGAc                                                            <  1:1102902/149‑1 (MQ=255)                                                                                                agaAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATg                                                       >  1:380473/1‑149 (MQ=255)                                                                                                agaAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATg                                                       >  1:176342/1‑149 (MQ=255)                                                                                                 gaAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGa                                                      <  1:104477/149‑1 (MQ=255)                                                                                                    aTTGGAACGCCAATTTCTACAGCCGCTGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATaa                                                   <  2:727794/149‑1 (MQ=255)                                                                                                           cGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCg                                            <  2:910980/149‑1 (MQ=255)                                                                                                             ccAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAGTACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTTAGATTTTTAAGTAAACATTAGCTGTGATTAATGATAAAGAAGCGCt                                          <  2:74684/149‑1 (MQ=18)                                                                                                             ccAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCt                                          <  2:1030879/149‑1 (MQ=255)                                                                                                             ccAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCt                                          <  1:730405/149‑1 (MQ=255)                                                                                                              cAATTTCTTCATATAATCCGAGTTGGTTTGAGCCCCAACATAAAAAACGTCTTTTTCTTTACAAATTAGTATTTCTTATGCATGAGTGTAACTCATTCATTCATATTTTTAAGTACACACTAGCTGTGACTAATGATAAAGAATCGCTa                                         <  2:709591/149‑1 (MQ=17)                                                                                                              cAATTTCTACAGACAATGCAAGTTTGCGTTGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCTa                                         <  2:93085/149‑1 (MQ=255)                                                                                                               aaTTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCTAc                                        >  1:989155/1‑149 (MQ=255)                                                                                                                aTTTCTACAGACTATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCTACa                                       <  2:1103730/149‑1 (MQ=255)                                                                                                                aTTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCTACa                                       <  2:1103715/149‑1 (MQ=255)                                                                                                                 tttCTACAGACAATCCAAGTTTGAGTGCGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCTACAt                                      <  1:648652/149‑1 (MQ=255)                                                                                                                    cTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCTACATaat                                   <  1:605395/149‑1 (MQ=255)                                                                                                                         gACAATGCAAGTTTGGGTGTGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATCCATTCATATTTTTAAGTACCCAGTAGCTGTGACTAATGATAAAGAATCGCTACATaatcaatc                              <  2:63697/149‑1 (MQ=18)                                                                                                                          acacTGCACGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCTACATAatcaatca                             <  1:978124/149‑1 (MQ=255)                                                                                                                           cAATGCAAGTTGGGGTGGGCCCCAACCTCCAGCATCACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCTACATAATCcatcat                            >  2:516248/1‑149 (MQ=18)                                                                                                                           cAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATCTTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCTACATAATCAATCAt                            >  1:452345/1‑149 (MQ=255)                                                                                                                           cAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCTACATAATCAATCAt                            >  2:590255/1‑149 (MQ=255)                                                                                                                           cAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCTACATAATAAATCAt                            >  2:701985/1‑149 (MQ=255)                                                                                                                                cAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTTTTTTTTTTTTAGTATTTCTTATGCATGAGTGTACCTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTCATGATAAAGAATCGCTACATAATCAATCATTAGTc                       >  2:919972/1‑149 (MQ=18)                                                                                                                                cAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCTACATAATCAATAATTAGTc                       >  2:92139/1‑149 (MQ=255)                                                                                                                                   gTTTGGGGGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTCGCTGTGACTAATGATAAAGATTTGCTACATAATTAAGCAGGAGTCGtt                    <  2:101242/149‑1 (MQ=17)                                                                                                                                   gTTGGGGTGGGCCCCATCATATGGTAATACTTTTTCTTTAGCAATTAGTATTTCTTATGCATGGGTGTGACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATGAAGAATCGCTACATAATCATTCATTAGTCGtt                    <  2:671115/149‑1 (MQ=18)                                                                                                                                   gTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCTACATAATCAATCATTAGTCGtt                    <  2:501147/149‑1 (MQ=255)                                                                                                                                     tGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCTACATAATCAATCATTAGTCGTTCt                  <  1:201113/149‑1 (MQ=255)                                                                                                                                     tGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCTACATAATCAATCATTAGTCGTTCt                  <  2:274596/149‑1 (MQ=255)                                                                                                                                      ggggTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTACGTACACATTTGCTGTGACTAATGATAAAGAATCGCTACATAATCAATCATTAGTCGTTCtt                 <  2:460622/149‑1 (MQ=255)                                                                                                                                      ggggTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCTACATAATCAATCATTAGTCGTTCtt                 <  2:36492/149‑1 (MQ=255)                                                                                                                                                ccAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCCTGAGTGTAACTCCTGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCTACATAATCAATCATTAGCCGTTCTTTTTCATTTcc       >  1:1048298/1‑149 (MQ=255)                                                                                                                                                     aTAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCTACATAATCAATCATTAGTCGTTCTTTATCGTTTCCGTccc  >  1:925357/1‑149 (MQ=255)                                                                                                                                                     aTAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCTACATAATCAATCATTAGTCGTTCTTTATCATTTCCGTccc  <  1:943403/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     ATAGGCTATAAAGTAATTGTGACTGAGATGAACTTTTATGTCTTAGACACTACAACACTATATTGGCAGTAGTTGACTGCGGGGCCCCAACATAGAGAAATTGGAACGCCAATTTCTACAGACAATGCAAGTTGGGGTGGGCCCCAACATAAAGAAATACTTTTTCTTTAGAAATTAGTATTTCTTATGCATGAGTGTAACTCATGCATTCATATTTTTAAGTACACATTAGCTGTGACTAATGATAAAGAATCGCTACATAATCAATCATTAGTCGTTCTTTATCATTTCCGTCCC  >  USA300TCH1516_ALE/801800‑802096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.