New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE = 67253646 (1.290)3 (0.100) 3/234 3.0 7.9% intergenic (+138/‑241) nth_1/btuF Endonuclease III/Vitamin B12‑binding protein
?USA300TCH1516_ALE 672603 = 32 (1.070)intergenic (+205/‑174) nth_1/btuF Endonuclease III/Vitamin B12‑binding protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ACCTTACTTTAAAAAGTAAACGCTGTGAAGTTAGATAGATGAGTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  USA300TCH1516_ALE/672380‑672536
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttgtttgtgcagcaactgcataaAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACAACTGCATAAAAGCCTCTAATCGCTAAAGATGAAGAGGAACGCAGTTGGATGCTACCGCACAACTGCATAAATCCCTCTAATCGCTA  >  USA300TCH1516_ALE/672603‑672740
                                                                                                                                                                                                                                                                                                       
ACCTTACTTTAAAAAGTAAACGCTGTGAAGTTAGATAGATGAGTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTG                                                                                                                                                            >  2:149389/1‑141
  ctaacaTTAAAAAGTAAACGCTGTGAAGTTAGATAGATGAGTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTG                                                                                                                                                          <  2:361795/135‑1
    TACTTTAAAAAGTAAACGCTGTGAAGTTAGATAGATGAGTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCA                                                                                                                                                        >  1:242338/1‑141
    TACTTTAAAAAGTAAACGCTGTGAAGTTAGATAGATGAGTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCA                                                                                                                                                        >  2:83359/1‑141
    TACTTTAAAAAGTAAACGCTGTGAAGTTAGATAGATGAGTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCA                                                                                                                                                        >  2:121914/1‑141
    TACTTTAAAAAGTAAACGCTGTGAAGTTAGATAGATGAGTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCA                                                                                                                                                        >  2:345141/1‑141
    TACTTTAAAAAGTAAACGCTGTGAAGTTAGATAGATGAGTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCA                                                                                                                                                        >  2:327844/1‑141
       TTTAAAAAGTAAACGCTGTGAAGTTAGATAGATGAGTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATACTACATAGACGCATACAAAATAAAAAACAGTTGTTTGTTTGTGCAGCA                                                                                                                                                     <  2:18734/141‑1
          AAAAAGTAAACGCTGTGAAGTTAGATAGATGAGTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACT                                                                                                                                                  >  2:62903/1‑141
              AGTAAACGCTGTGAAGTTAGATAGATGAGTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCAT                                                                                                                                              <  1:234337/141‑1
              AGTAAACGCTGTGAAGTTAGATAGATGAGTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCAT                                                                                                                                              >  1:74168/1‑141
                                          GTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGA                                                                                                                  >  2:284677/1‑141
                                                                    TACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACA                                                                                        <  1:404783/141‑1
                                                                                                TATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACAACTGCATAAATCCCTCTAATCGCTAAAG                                                            <  1:284677/141‑1
                                                                                                                                                  CAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACAACTGCATAAAAGCCTCTAATCGCTAAAGATGAAGAGGAACGCAGTTGGATGCTACCGCACAACTGCATAAATCCCTCT          >  1:1421/1‑141
                                                                                                                                                  CAACTACATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACAACTGCATAAAAGCCTCTAATCGCTAAAGATGAAGAGGAACGCAGTTGGATGCTACCGCACAACTGCATAAATCCCTCT          >  1:215790/1‑141
                                                                                                                                                   tttttCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACAACTGCATAAAAGCCTCTAATCGCTAAAGATGAAGAGGAACGCAGTTGGATGCTACCGCACAACTGCATAAATCCCTCTA         >  2:299728/6‑141
                                                                                                                                                   AACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACAACTGCATAAAAGCCTCTAATCGCTAAAGATGAAGAGGAACGCAGTTGGATGCTACCGCACAACTGCATAAATCACTCTA         >  2:330989/1‑141
                                                                                                                                                        CATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACAACTGCATAAAAGCCTCTAATCGCTAAAGATGAAGAGGAACGCAGTTGGATGCTACCGCACAACTGCATAAATCCCTCTAATCGC    <  2:359499/141‑1
                                                                                                                                                        CATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACAACTGCATAAAAGCCTCTAATCGCTAAAGATGAAGAGGAACGCAGTTGGATGCTACCGCACAACTGCATAAATCCCTCTAATCGC    <  2:45738/141‑1
                                                                                                                                                          TAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACAACTGCATAAAAGCCTCTAATCGCTAAAGATGAAGAGGAACGCAGTTGGATGCTACCGCACAACTGCATAAATCCCTCTAATCGCTA  <  2:284830/141‑1
                                                                                                                                                          TAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACAACTGCATAAAAGCCTCTAATCGCTAAAGATGAAGAGGAACGCAGTTGGATGCTACCGCACAACTGCATAAATCCCTCTAATCGCTA  <  2:201296/141‑1
                                                                                                                                                          TAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACAACTGCATAAAAGCCTCTAATCGCTAAAGATGAAGAGGAACGCAGTTGGATGCTACCGCACAACTGCATAAATCCCTCTAATCGCTA  >  1:77559/1‑141
                                                                                                                                                                                                                                                                                                       
ACCTTACTTTAAAAAGTAAACGCTGTGAAGTTAGATAGATGAGTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  USA300TCH1516_ALE/672380‑672536
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttgtttgtgcagcaactgcataaAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACAACTGCATAAAAGCCTCTAATCGCTAAAGATGAAGAGGAACGCAGTTGGATGCTACCGCACAACTGCATAAATCCCTCTAATCGCTA  >  USA300TCH1516_ALE/672603‑672740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.