New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	USA300TCH1516_ALE	= 672536	46 (1.290)	3 (0.100)	3/234	3.0	7.9%	intergenic (+138/‑241)	nth_1/btuF	Endonuclease III/Vitamin B12‑binding protein
	?	USA300TCH1516_ALE	672603 =	32 (1.070)					intergenic (+205/‑174)	nth_1/btuF	Endonuclease III/Vitamin B12‑binding protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCTGTGAAGTTAGATAGATGAGTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACAACTGCATAAAAGCCTCTAATCG  >  USA300TCH1516_ALE/672401‑672676                                                                                                                                        |                                                                                                                                             gCTGTGAAGTTAGATAGATGAGTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGcc                                                                                                                                         >  2:298482/1‑141 (MQ=255)  cTGTGAAGTTAGATAGATGAGTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGccc                                                                                                                                        <  2:267923/141‑1 (MQ=255)       aaGTTAGATAGATGAGTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAAt                                                                                                                                   >  2:71088/1‑141 (MQ=255)            agatagatGAGTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTa                                                                                                                              <  1:271361/141‑1 (MQ=255)            agatagatGAGTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGCTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTa                                                                                                                              >  2:367106/1‑141 (MQ=255)                      gTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAgg                                                                                                                    <  2:159502/141‑1 (MQ=255)                      gTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAgg                                                                                                                    <  2:266036/141‑1 (MQ=255)                      gTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAggtgaaga                                                                                                                    >  2:284677/1‑134 (MQ=37)                           tatGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAga                                                                                                               >  1:76399/1‑140 (MQ=255)                             tGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTaa                                                                                                             <  1:149389/141‑1 (MQ=255)                                     aaaaaaTAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATAc                                                                                                     <  1:91439/141‑1 (MQ=255)                                        aaaTAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAgt                                                                                                  >  2:409265/1‑141 (MQ=255)                                        aaaTAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAgt                                                                                                  >  2:10196/1‑141 (MQ=255)                                         aataatTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAgtt                                                                                                 <  1:71088/141‑1 (MQ=255)                                               ttACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGttgtttgt                                                                                           >  2:372086/1‑141 (MQ=255)                                               ttACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGttgtttgt                                                                                           >  1:172403/1‑141 (MQ=255)                                               ttACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGttgtttgt                                                                                           >  2:245102/1‑141 (MQ=255)                                               ttACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGATGTTTGTTTGTGCAGAAACTGCATGAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGttgtttgt                                                                                           >  1:121372/1‑141 (MQ=255)                                                tACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTtgtg                                                                                          >  2:55569/1‑141 (MQ=255)                                                  cTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTgca                                                                                        <  1:301816/141‑1 (MQ=255)                                                         cTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTg                                                                                 <  2:214632/141‑1 (MQ=255)                                                         cTTTTAGTATGTGGACTTATATAATAAAGAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTg                                                                                 <  1:15377/141‑1 (MQ=255)                                                         cTTGTAGTATGTGGACTTATATAATAAATAGGAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTg                                                                                 <  2:95811/141‑1 (MQ=255)                                                                            tataATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCAc                                                              >  2:126965/1‑141 (MQ=255)                                                                            tataATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCAc                                                              <  2:242338/141‑1 (MQ=255)                                                                            tataATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCAc                                                              <  1:83359/141‑1 (MQ=255)                                                                            tataATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCAc                                                              <  1:327844/141‑1 (MQ=255)                                                                            tataATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCAc                                                              <  1:345141/141‑1 (MQ=255)                                                                            tataATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAAGCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCAc                                                              <  1:121914/141‑1 (MQ=255)                                                                                           aatatAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGa                                               <  2:280766/140‑1 (MQ=255)                                                                                                 aaGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAg                                         >  1:246824/1‑141 (MQ=255)                                                                                                    aaaaaaaCAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTg                                      <  2:111610/141‑1 (MQ=255)                                                                                                    aaaaaaaCAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTg                                      <  2:126026/141‑1 (MQ=255)                                                                                                       aaaaCAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGAt                                   <  1:298482/141‑1 (MQ=255)                                                                                                        aaaCAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATg                                  <  1:377275/141‑1 (MQ=255)                                                                                                         aaCAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGc                                 >  1:359499/1‑141 (MQ=255)                                                                                                         aaCAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGc                                 >  1:45738/1‑141 (MQ=255)                                                                                                            aGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTaa                              <  2:148917/141‑1 (MQ=255)                                                                                                            aGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTaa                              <  1:131209/141‑1 (MQ=255)                                                                                                                      ttgtGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACAACTg                    <  1:372086/141‑1 (MQ=255)                                                                                                                      ttgtGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACAACTg                    <  1:245102/141‑1 (MQ=255)                                                                                                                       tgtgCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACAACTGc                   >  1:321674/1‑141 (MQ=255)                                                                                                                              caACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGGAGAGGAACGCAGTTGGATGCTAAGGCACAACTGCATAAAAg            >  1:207026/1‑141 (MQ=255)                                                                                                                              caACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACAACTGCATAAAAg            >  1:291586/1‑141 (MQ=255)                                                                                                                              caACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACAACTGCATAAAAg            >  2:377553/1‑141 (MQ=255)                                                                                                                                        agagCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCCTAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACAACTGCATAAAAGCCTCTAATCg  >  1:9266/1‑141 (MQ=255)                                                                                                                                        agagCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACAACTGCATAAAAGCCTCTAATCg  >  1:147456/1‑141 (MQ=255)                                                                                                                                        |                                                                                                                                             GCTGTGAAGTTAGATAGATGAGTTTATATGAAATATAAAAAATAATTTACTATTTTCTTTTAGTATGTGGACTTATATAATAAATAGAAGCATATAAAGAAAAAAACAGTTGTTTGTTTGTGCAGCAACTGCATAAGAGCCCCTAATCGCTAAAGCTCAAGGGGAGTAAAGGAATACAGTTGTTTGTGCAGCAACTGCATAAAAGCCTCTAATCACTAAAGGTGAAGAGGAACGCAGTTGGATGCTAAGGCACAACTGCATAAAAGCCTCTAATCG  >  USA300TCH1516_ALE/672401‑672676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.