breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsSNFM_1_15_0_S199_S1996_L008_R1_001.good.fq712,41398,392,832100.0%138.1 bases141 bases88.6%
errorsSNFM_1_15_0_S199_S1996_L008_R2_001.good.fq712,41398,392,832100.0%138.1 bases141 bases87.0%
total1,424,826196,785,664100.0%138.1 bases141 bases87.8%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionCP0007302,872,91559.61.7100.0%Staphylococcus aureus subsp. aureus USA300_TCH1516, complete genome.
total2,872,915100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 10000026394
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 3
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 500020
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.002

Junction Skew Score Calculation

reference sequencepr(no read start)
CP0007300.82341

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 0
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 6

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoffOFF
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff2
Polymorphism frequency cutoff0.05
Polymorphism minimum variant coverage each strand2
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs of ≥3 bases
Skip base substitutions when they create a homopolymer flanked on each side by ≥5 bases

Software Versions

programversion
bowtie22.3.4.3
R3.5.1

Execution Times

stepstartendelapsed
Read and reference sequence file input17:22:14 26 Oct 201817:22:40 26 Oct 201826 seconds
Read alignment to reference genome17:22:41 26 Oct 201817:25:45 26 Oct 20183 minutes 4 seconds
Preprocessing alignments for candidate junction identification17:25:45 26 Oct 201817:26:07 26 Oct 201822 seconds
Preliminary analysis of coverage distribution17:26:07 26 Oct 201817:26:41 26 Oct 201834 seconds
Identifying junction candidates17:26:41 26 Oct 201817:26:49 26 Oct 20188 seconds
Re-alignment to junction candidates17:26:49 26 Oct 201817:27:33 26 Oct 201844 seconds
Resolving best read alignments17:27:33 26 Oct 201818:11:38 26 Oct 201844 minutes 5 seconds
Creating BAM files18:11:38 26 Oct 201818:12:08 26 Oct 201830 seconds
Tabulating error counts18:12:08 26 Oct 201819:31:21 26 Oct 20181 hour 19 minutes 13 seconds
Re-calibrating base error rates19:31:21 26 Oct 201819:31:23 26 Oct 20182 seconds
Examining read alignment evidence19:31:23 26 Oct 201803:43:31 27 Oct 20188 hours 12 minutes 8 seconds
Polymorphism statistics03:43:31 27 Oct 201803:43:32 27 Oct 20181 second
Output03:43:32 27 Oct 201803:43:41 27 Oct 20189 seconds
Total 10 hours 21 minutes 26 seconds