Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	CP000730	1,302,967	Δ4 bp	100%	coding (325‑328/714 nt)	USA300HOU_1209 →	possible GntR family transcriptional regulator

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	CP000730	1302967	1302970	4	74 [0]	[1] 75	USA300HOU_1209	possible GntR family transcriptional regulator

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	= 1302966	0 (0.000)	70 (1.120)	49/256	NT	100%	coding (324/714 nt)	USA300HOU_1209	possible GntR family transcriptional regulator
	?	CP000730	1302971 =	0 (0.000)					coding (329/714 nt)	USA300HOU_1209	possible GntR family transcriptional regulator

AAGAGGGCACAAGTGTTAAGTCACTGCCCCCTTTCTTTTATCCATTGAATGAACTTATAAGTATTGGGCAAATGATTAAAAATGCAGGATTTGAATGCGGAACTGAATACTTAAATTTTGATGAGCAACCAGCAACTATGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTAAAAAAGAATTAACATGTACAGAGTAT  >  CP000730/1302833‑1303107                                                                                                                                           |                                                                                                                                         tttgaagagggcacaagtgttaagtcactgccccctttcttttatccattgaatgaacttataagtattgggcaaataattaaaaaaacaggatttaaatgcggaactgaatacttaaattttgatgagcaaccatcaTg                                                                                                                                         >  1:59031‑M2/139‑140 (MQ=255)      agagggcacaagtgttaagtcactgccccctttcttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGa                                                                                                                                    <  1:67608‑M2/7‑1 (MQ=255)         gggcacaagtgttaagtcactgccccctttcttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGc                                                                                                                                 <  1:638443‑M2/10‑1 (MQ=255)         gggcacaagtgttaagtcacagccccctttcttttatccattgaatgaacttataagtcttgggcaaatgattaaaaatgcaggatttgaatgcggaaccgaatacttaaatttagatgagcaaccagcaTGTtagaaac                                                                                                                                 >  1:443354‑M2/131‑137 (MQ=255)           gcacaagtgttaagtcactgccccctttcttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcatgatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCaa                                                                                                                               <  2:306854‑M2/12‑1 (MQ=255)             acaagtgttaagtcactgccccctttcttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAAt                                                                                                                             >  2:385627‑M2/127‑140 (MQ=255)              caagtgttaagtcactgccccctttcttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaagcagcatgataggagcacaac                                                                                                                            >  2:646078‑M2/126‑131 (MQ=255)                agtgttaagtcactgcccgcttccttttatccattgaatgaacttataagtatggggaaatggattaaaaatgcaggatgtgaatgcggaactgaatacttaaattttgatgaggaaccagcaTGTTAGATGCAAATttg                                                                                                                          <  2:459220‑M2/17‑1 (MQ=255)                agtgttaagtcactgccccctttcttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATttg                                                                                                                          <  1:365083‑M2/17‑1 (MQ=255)                 gtgttaagtcactgccccctttcttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggatctgaatacttaaattttgatgagcaaccagcaTGTTAGATGAAAATttgt                                                                                                                         <  2:535962‑M2/18‑1 (MQ=255)                   gttaagtcactgccccctttcttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATttgttg                                                                                                                       <  1:47093‑M2/20‑1 (MQ=255)                   gttaagtcactgccccctttcttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATttgttg                                                                                                                       <  2:129269‑M2/20‑1 (MQ=255)                       agtcactgccccctttcttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCg                                                                                                                   <  1:427695‑M2/24‑1 (MQ=255)                            ctgccccctttcttttatcccttgaatgaacttataagtatggggcaaatgagtaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTgaa                                                                                                              >  1:145442‑M2/112‑140 (MQ=255)                            ctgccccctttcttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAACTTTGTTGAGCGTTgaa                                                                                                              <  1:363366‑M2/29‑1 (MQ=255)                               ccccctttcttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattntgatgagcaaccagcaTGGTAGATGCAAATTTGTTGAGCATTgaagaa                                                                                                           >  1:614391‑M2/109‑140 (MQ=255)                                cccctttcttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTAaagaag                                                                                                          >  1:118959‑M2/108‑140 (MQ=255)                                    tttcttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGata                                                                                                      <  1:156977‑M2/37‑1 (MQ=255)                                    tttctttgatccattgaatggcctgagaagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGata                                                                                                      <  1:163187‑M2/37‑1 (MQ=255)                                     ttttgttatccattgaatgaacttagaagtattgtgaaaatgattaaaaatgcaggattggaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGatat                                                                                                     <  1:359837‑M2/38‑1 (MQ=255)                                     ttcttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGatat                                                                                                     <  1:94671‑M2/38‑1 (MQ=255)                                     ttcttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGatat                                                                                                     <  1:641096‑M2/38‑1 (MQ=255)                                        ttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCa                                                                                                  >  2:252553‑M2/100‑140 (MQ=255)                                        ttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaacagaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATATGTTGAGCGTTGAAGAAGGATAGCCa                                                                                                  >  2:433211‑M2/100‑140 (MQ=255)                                         tttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaattttgttttaccaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAg                                                                                                 >  1:345778‑M2/99‑140 (MQ=255)                                           tatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCGGTa                                                                                               >  1:91321‑M2/97‑140 (MQ=255)                                               cattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaaatttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCa                                                                                           >  1:265162‑M2/93‑140 (MQ=255)                                               cattgaatgaacttacaagcattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCa                                                                                           >  1:541617‑M2/93‑140 (MQ=255)                                               cattgaatgaaattataagtattgggcaaatgattaaaaatgcaggatttgaaagcggaaatgaatacttaaattttgatgagcaacaagcaTGTTAGATGCAAATTTGTTGCGCGTTGAAGAAGGATATACAGTAAACa                                                                                           >  2:176615‑M2/93‑140 (MQ=255)                                               cattgaatcaacttagaattattaggcaaatgattaaaaatgcagcatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTGGAGCGTTGAAGAAGGATATCCAGTAACCa                                                                                           <  2:149228‑M2/48‑1 (MQ=255)                                                 ttgaatgaacttataagtattgggcaaatgattaaaaatgcagggtttgaatgcggaactgaatacttaaattttgatgaacaaccagcaTGTTAAATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCATTAACCAtt                                                                                         >  1:694921‑M2/91‑140 (MQ=255)                                                 ttgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGAAATCCAGTAACCAtt                                                                                         >  1:25410‑M2/91‑140 (MQ=255)                                                     atgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAg                                                                                     <  1:131648‑M2/54‑1 (MQ=255)                                                        aacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAAc                                                                                  >  1:490771‑M2/84‑140 (MQ=255)                                                        aacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAAc                                                                                  >  1:273809‑M2/84‑140 (MQ=255)                                                        aacttataagtattgggaaaatgattaaaaatgcaggatttgaatgcggaacggaatacttaaattttgatgagcaaccatcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAAc                                                                                  <  2:684927‑M2/57‑1 (MQ=255)                                                         acttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACg                                                                                 >  1:273202‑M2/83‑140 (MQ=255)                                                                    ttgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTCTAGAACGATTACGAACTg                                                                      >  2:47637‑M2/72‑140 (MQ=255)                                                                    ttgggcaaatgatcaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTg                                                                      <  2:167623‑M2/69‑1 (MQ=255)                                                                        gcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGa                                                                  >  2:726123‑M2/68‑140 (MQ=255)                                                                        gcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATATAATGAGCGTTGAAGAAGGATATCCAGTAACAATTATAGAACGGTTACGAACTGCCGa                                                                  >  1:137953‑M2/68‑140 (MQ=255)                                                                            atgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTCCGAACTGCCAAGGga                                                              >  2:46214‑M2/64‑140 (MQ=255)                                                                             tgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGATAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGgag                                                             >  1:665115‑M2/63‑140 (MQ=255)                                                                             tgaatcaaaattcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGgag                                                             <  1:163148‑M2/78‑1 (MQ=255)                                                                              gattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTAAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCATTAACCATTATAGAACGATTACAAACTAGCGAtggaaa                                                            >  2:414398‑M2/62‑138 (MQ=255)                                                                               attaaaaatgcaggatttgaatgcggaactgaatacttagattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGaa                                                           <  1:513801‑M2/80‑1 (MQ=255)                                                                               attaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGccgatgtggaa                                                           >  2:138099‑M2/61‑135 (MQ=255)                                                                               atcaaaaatgcaggatttgaatgcggaactgaatacttaaattgtgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAATAAGGATATCCAGTAACCATCATAGACCGCTTACTAACTGCCGATGGAGaa                                                           <  2:156410‑M2/80‑1 (MQ=255)                                                                               aataaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAACGAGATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGaa                                                           <  2:298181‑M2/80‑1 (MQ=255)                                                                                ttaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAAc                                                          >  1:611905‑M2/60‑140 (MQ=255)                                                                                 taaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATAGCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAAcc                                                         <  1:383969‑M2/82‑1 (MQ=255)                                                                                      atgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGgtcg                                                    <  2:677921‑M2/87‑1 (MQ=255)                                                                                         caggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGCACCGGTCGTCt                                                 >  2:184138‑M2/51‑140 (MQ=255)                                                                                         caggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGACCCGGTCGTCt                                                 >  1:726130‑M2/51‑140 (MQ=255)                                                                                                 gaatgcggaactgaatacttaaattttgatgagcaaccagcaTGGTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTa                                         <  1:376834‑M2/98‑1 (MQ=255)                                                                                                   atgcggaactgaatacttntattttgatgagcaacctgcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAGTCATTCGAAAACTATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGa                                       <  1:646078‑M2/100‑1 (MQ=255)                                                                                                      cggaattgaatagttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATCATAGAACGATTACGAACTGCCGAGGGAGAACCGGTCGTCTATTGTTTATATaa                                    <  2:443354‑M2/103‑1 (MQ=255)                                                                                                         aactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAAt                                 <  2:577508‑M2/106‑1 (MQ=255)                                                                                                             gaattcttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCt                             <  1:101218‑M2/110‑1 (MQ=255)                                                                                                              aatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTa                            >  2:393485‑M2/30‑140 (MQ=255)                                                                                                                  cttaaatttttatgagcaaccagcaTGTTAGATGCAAATTGGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTaaaaa                        <  2:232961‑M2/115‑1 (MQ=255)                                                                                                                         tttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGACGAAGGCTATCCAGTCACCATTATAGAACGATTACGAACTGATGATGTACAACCGTTCGTCAATTGTTTAGATAAAATTGCTTAAATAGAATTa                 >  1:275559‑M2/19‑140 (MQ=255)                                                                                                                         tttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTAAAAAAGAATTa                 >  2:493041‑M2/19‑140 (MQ=255)                                                                                                                         tttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTAAGAATTGTCTTAGAAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTAAAAAAGAATTa                 >  1:680577‑M2/19‑140 (MQ=255)                                                                                                                          ctgatggccaacccgcgTGTTAGATGTATATTTGTTTAGCGTTGAAGAATGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTAAAAAAGAATTaa                <  2:498291‑M2/123‑1 (MQ=255)                                                                                                                            gatgagtaacgagcaTGTTAGATGGAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTCCGACCTGTCGAAGGAGAACCGGTCGTCTATTGTTTAGATAAAATAAATAAAAAAGAATTAACa              <  2:545689‑M2/125‑1 (MQ=255)                                                                                                                            gatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTAAAACAGAATTAACa              >  2:428784‑M2/16‑140 (MQ=255)                                                                                                                            gatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTAAAAAAGAATTAACa              <  2:345778‑M2/125‑1 (MQ=255)                                                                                                                            gatgagcaaacagcaTGTCAGATGCGAAGTGTTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTAAAAAAGAATTAACa              <  2:74862‑M2/125‑1 (MQ=255)                                                                                                                            aagcagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTGAGCTATATTTAAAATTGATATAAAAGAATTAACa              <  2:666148‑M2/125‑1 (MQ=255)                                                                                                                                    accagcaTGTTCGATGCACATTTGTTGCGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTAAAAAAGAATTAACATGTAcagg      >  1:661033‑M2/8‑139 (MQ=255)                                                                                                                                     ccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTAAAAAAGAATTAACATGTACagag     >  1:451850‑M2/7‑140 (MQ=255)                                                                                                                                     ccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTAAAAAAGAATTAAAATGTACagag     >  1:451950‑M2/7‑140 (MQ=255)                                                                                                                                      cagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTAAAAAAGAATTAACATGTACAGAGt    <  1:726123‑M2/135‑1 (MQ=255)                                                                                                                                        gtATGTTAGTGGCAAATTTGTTGAGCGTTGAAGAAGGATATGCAGTAACCATTACAGATCGATTTCGAACTGCCGATGGAGAACCGGTAGTCTATTGTTTAGATAAAATTGCTAGAAAAGAATTAACATGTACAGAGTAt  <  2:432519/139‑1 (MQ=255)                                                                                                                                           |                                                                                                                                         AAGAGGGCACAAGTGTTAAGTCACTGCCCCCTTTCTTTTATCCATTGAATGAACTTATAAGTATTGGGCAAATGATTAAAAATGCAGGATTTGAATGCGGAACTGAATACTTAAATTTTGATGAGCAACCAGCAACTATGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTAAAAAAGAATTAACATGTACAGAGTAT  >  CP000730/1302833‑1303107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑