Predicted mutation
evidence seq id position mutation annotation gene description
MC JC CP000730 1,302,967 Δ4 bp coding (325‑328/714 nt) USA300HOU_1209 → possible GntR family transcriptional regulator

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ CP000730 1302967 1302970 4 22 [0] [0] 22 USA300HOU_1209 possible GntR family transcriptional regulator

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 = 13029660 (0.000)22 (0.650) 22/276 0.8 100% coding (324/714 nt) USA300HOU_1209 possible GntR family transcriptional regulator
?CP000730 1302971 = 0 (0.000)coding (329/714 nt) USA300HOU_1209 possible GntR family transcriptional regulator

TGTTAAGTCACTGCCCCCTTTCTTTTATCCATTGAATGAACTTATAAGTATTGGGCAAATGATTAAAAATGCAGGATTTGAATGCGGAACTGAATACTTAAATTTTGATGAGCAACCAGCAACTATGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTAAAAAAGAATTAACATGTACAGAGTA  >  CP000730/1302846‑1303106
                                                                                                                             |                                                                                                                                       
caagtgttaagtcactgccccctttcttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAAttt                                                                                                                          <  1:121586‑M2/16‑1 (MQ=255)
      ttaagtcactgccccctttcttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAg                                                                                                                    >  1:61057‑M2/120‑141 (MQ=255)
       taagtcactgccccctttcttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGc                                                                                                                   <  1:116855‑M2/23‑1 (MQ=255)
               tgccccctttcttttatccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTgaaga                                                                                                           >  1:122102‑M2/111‑141 (MQ=255)
                               tccattgaatgaacttataagtattgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAAcc                                                                                           <  2:72319‑M2/47‑1 (MQ=255)
                                                       tgggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGcc                                                                   >  1:223894‑M2/71‑141 (MQ=255)
                                                        gggcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCg                                                                  <  2:114637‑M2/72‑1 (MQ=255)
                                                          gcaaatgattaaaaatgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGAt                                                                >  2:92226‑M2/68‑141 (MQ=255)
                                                                        atgcaggatttgaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGgtcgt                                                  <  1:175554‑M2/88‑1 (MQ=255)
                                                                                tttgaatgcgtaactgaatacttaaattttgatgatcaaccatcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTCTGACCTGCCGATTTAGAACCGGTCGTCTATTGtt                                          <  2:183571‑M2/96‑1 (MQ=255)
                                                                                   gaatgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAg                                       >  2:319496‑M2/43‑141 (MQ=255)
                                                                                     atgcggaactgaatacttaaattttgatgagcaaccagcaTGTTAGCTGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGAt                                     >  1:307540‑M2/41‑141 (MQ=255)
                                                                                       gcggaactgaatacttaaattttgatgatcaaccagcaTGTTAGATGCAAAGTTGTTGAACGTTGAAGAAGGATATTCAGTAACCATTATAGAACGATTACGAACTTCCGATGGAGAACCGGTCGTCTATTGTTTAGATaa                                   >  1:35896‑M2/39‑141 (MQ=255)
                                                                                           aactgaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAAtt                               <  1:173726‑M2/107‑1 (MQ=255)
                                                                                               gaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTa                           >  1:36627‑M2/31‑141 (MQ=255)
                                                                                               gaatacttaaattttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTa                           <  2:241437‑M2/111‑1 (MQ=255)
                                                                                                         attttgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTAAAAAAGAAtt                 >  1:60536‑M2/21‑141 (MQ=255)
                                                                                                          ttatgatgagcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTAAAAAAGAATTa                <  2:317613‑M2/122‑1 (MQ=255)
                                                                                                               atgaccaaccagcaTGTTAGATGCAAATTTGTTGAGCGTCGAAAAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACAGGTCGTCTATTGTTTATATAAAATTGCTAAAAAAGAATTAACATg           <  2:170886‑M2/127‑1 (MQ=255)
                                                                                                                   gcaaccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTAAAAAAGAATTAACATGTACa       >  2:52416‑M2/11‑141 (MQ=255)
                                                                                                                       ccagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTAAAAAAGAATTAACATGTACAGAGt   <  1:266738‑M2/135‑1 (MQ=255)
                                                                                                                        cagcaTGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTAAAAAAGAATTAACATGTACAGAGTa  >  1:36331‑M2/6‑141 (MQ=255)
                                                                                                                             |                                                                                                                                       
TGTTAAGTCACTGCCCCCTTTCTTTTATCCATTGAATGAACTTATAAGTATTGGGCAAATGATTAAAAATGCAGGATTTGAATGCGGAACTGAATACTTAAATTTTGATGAGCAACCAGCAACTATGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTAAAAAAGAATTAACATGTACAGAGTA  >  CP000730/1302846‑1303106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.