Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	CP000730	1,302,967	Δ4 bp	coding (325‑328/714 nt)	USA300HOU_1209 →	possible GntR family transcriptional regulator

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	CP000730	1302967	1302970	4	22 [0]	[0] 22	USA300HOU_1209	possible GntR family transcriptional regulator

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	= 1302966	0 (0.000)	22 (0.650)	22/276	0.8	100%	coding (324/714 nt)	USA300HOU_1209	possible GntR family transcriptional regulator
	?	CP000730	1302971 =	0 (0.000)					coding (329/714 nt)	USA300HOU_1209	possible GntR family transcriptional regulator

CAAGTGTTAAGTCACTGCCCCCTTTCTTTTATCCATTGAATGAACTTATAAGTATTGGGCAAATGATTAAAAATGCAGGATTTGAATGCGGAACTGAATACTTAAATTTTGATGAGCAACCAGCAACTATGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTAAAAAAGAATTAACATGTACAG  >  CP000730/1302842‑1303102                                                                                                                             |                                                                                                                                         caAGTGTTAAGTCACTGCCCCCTTTCTTTTATCCATTGAATGAACTTATAAGTATTGGGCAAATGATTAAAAATGCAGGATTTGAATGCGGAACTGAATACTTAAATTTTGATGAGCAACCAGCAtgttagatgcaaattt                                                                                                                          <  1:121586‑M1/141‑17 (MQ=255)       ttAAGTCACTGCCCCCTTTCTTTTATCCATTGAATGAACTTATAAGTATTGGGCAAATGATTAAAAATGCAGGATTTGAATGCGGAACTGAATACTTAAATTTTGATGAGCAACCAGCAtgttagatgcaaatttgttgag                                                                                                                    >  1:61057‑M1/1‑119 (MQ=255)        tAAGTCACTGCCCCCTTTCTTTTATCCATTGAATGAACTTATAAGTATTGGGCAAATGATTAAAAATGCAGGATTTGAATGCGGAACTGAATACTTAAATTTTGATGAGCAACCAGCAtgttagatgcaaatttgttgagc                                                                                                                   <  1:116855‑M1/141‑24 (MQ=255)                tGCCCCCTTTCTTTTATCCATTGAATGAACTTATAAGTATTGGGCAAATGATTAAAAATGCAGGATTTGAATGCGGAACTGAATACTTAAATTTTGATGAGCAACCAGCAtgttagatgcaaatttgttgagcgttgaaga                                                                                                           >  1:122102‑M1/1‑110 (MQ=255)                                tCCATTGAATGAACTTATAAGTATTGGGCAAATGATTAAAAATGCAGGATTTGAATGCGGAACTGAATACTTAAATTTTGATGAGCAACCAGCAtgttagatgcaaatttgttgagcgttgaagaaggatatccagtaacc                                                                                           <  2:72319‑M1/141‑48 (MQ=255)                                                        tGGGCAAATGATTAAAAATGCAGGATTTGAATGCGGAACTGAATACTTAAATTTTGATGAGCAACCAGCAtgttagatgcaaatttgttgagcgttgaagaaggatatccagtaaccattatagaacgattacgaactgcc                                                                   >  1:223894‑M1/1‑70 (MQ=255)                                                         gggCAAATGATTAAAAATGCAGGATTTGAATGCGGAACTGAATACTTAAATTTTGATGAGCAACCAGCAtgttagatgcaaatttgttgagcgttgaagaaggatatccagtaaccattatagaacgattacgaactgccg                                                                  <  2:114637‑M1/141‑73 (MQ=255)                                                           gCAAATGATTAAAAATGCAGGATTTGAATGCGGAACTGAATACTTAAATTTTGATGAGCAACCAGCAtgttagatgcaaatttgttgagcgttgaagaaggatatccagtaaccattatagaacgattacgaactgccgat                                                                >  2:92226‑M1/1‑67 (MQ=255)                                                                         aTGCAGGATTTGAATGCGGAACTGAATACTTAAATTTTGATGAGCAACCAGCAtgttagatgcaaatttgttgagcgttgaagaaggatatccagtaaccattatagaacgattacgaactgccgatggagaaccggtcgt                                                  <  1:175554‑M1/141‑89 (MQ=255)                                                                                 tttGAATGCGTAACTGAATACTTAAATTTTGATGATCAACCATCAtgttagatgcaaatttgttgagcgttgaagaaggatatccagtaaccattatagaacgattctgacctgccgatttagaaccggtcgtctattgtt                                          <  2:183571‑M1/141‑97 (MQ=255)                                                                                    gAATGCGGAACTGAATACTTAAATTTTGATGAGCAACCAGCAtgttagatgcaaatttgttgagcgttgaagaaggatatccagtaaccattatagaacgattacgaactgccgatggagaaccggtcgtctattgtttag                                       >  2:319496‑M1/1‑42 (MQ=255)                                                                                      aTGCGGAACTGAATACTTAAATTTTGATGAGCAACCAGCAtgttagctgcaaatttgttgagcgttgaagaaggatatccagtaaccattatagaacgattacgaactgccgatggagaaccggtcgtctattgtttagat                                     >  1:307540‑M1/1‑40 (MQ=255)                                                                                        gCGGAACTGAATACTTAAATTTTGATGATCAACCAGCAtgttagatgcaaagttgttgaacgttgaagaaggatattcagtaaccattatagaacgattacgaacttccgatggagaaccggtcgtctattgtttagataa                                   >  1:35896‑M1/1‑38 (MQ=255)                                                                                            aaCTGAATACTTAAATTTTGATGAGCAACCAGCAtgttagatgcaaatttgttgagcgttgaagaaggatatccagtaaccattatagaacgattacgaactgccgatggagaaccggtcgtctattgtttagataaaatt                               <  1:173726‑M1/141‑108 (MQ=255)                                                                                                gAATACTTAAATTTTGATGAGCAACCAGCAtgttagatgcaaatttgttgagcgttgaagaaggatatccagtaaccattatagaacgattacgaactgccgatggagaaccggtcgtctattgtttagataaaattgcta                           >  1:36627‑M1/1‑30 (MQ=255)                                                                                                gAATACTTAAATTTTGATGAGCAACCAGCAtgttagatgcaaatttgttgagcgttgaagaaggatatccagtaaccattatagaacgattacgaactgccgatggagaaccggtcgtctattgtttagataaaattgcta                           <  2:241437‑M1/141‑112 (MQ=255)                                                                                                          aTTTTGATGAGCAACCAGCAtgttagatgcaaatttgttgagcgttgaagaaggatatccagtaaccattatagaacgattacgaactgccgatggagaaccggtcgtctattgtttagataaaattgctaaaaaagaatt                 >  1:60536‑M1/1‑20 (MQ=255)                                                                                                           ttatgatgagcaACCAGCAtgttagatgcaaatttgttgagcgttgaagaaggatatccagtaaccattatagaacgattacgaactgccgatggagaaccggtcgtctattgtttagataaaattgctaaaaaagaatta                <  2:317613‑M1/138‑123 (MQ=255)                                                                                                                atgaCCAACCAGCAtgttagatgcaaatttgttgagcgtcgaaaaaggatatccagtaaccattatagaacgattacgaactgccgatggagaacaggtcgtctattgtttatataaaattgctaaaaaagaattaacatg           <  2:170886‑M1/141‑128 (MQ=255)                                                                                                                    gCAACCAGCAtgttagatgcaaatttgttgagcgttgaagaaggatatccagtaaccattatagaacgattacgaactgccgatggagaaccggtcgtctattgtttagataaaattgctaaaaaagaattaacatgtaca       >  2:52416‑M1/1‑10 (MQ=255)                                                                                                                        ccAGCAtgttagatgcaaatttgttgagcgttgaagaaggatatccagtaaccattatagaacgattacgaactgccgatggagaaccggtcgtctattgtttagataaaattgctaaaaaagaattaacatgtacagagt   <  1:266738‑M1/141‑136 (MQ=255)                                                                                                                         cAGCAtgttagatgcaaatttgttgagcgttgaagaaggatatccagtaaccattatagaacgattacgaactgccgatggagaaccggtcgtctattgtttagataaaattgctaaaaaagaattaacatgtacagagta  >  1:36331‑M1/1‑5 (MQ=255)                                                                                                                             |                                                                                                                                         CAAGTGTTAAGTCACTGCCCCCTTTCTTTTATCCATTGAATGAACTTATAAGTATTGGGCAAATGATTAAAAATGCAGGATTTGAATGCGGAACTGAATACTTAAATTTTGATGAGCAACCAGCAACTATGTTAGATGCAAATTTGTTGAGCGTTGAAGAAGGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTATTGTTTAGATAAAATTGCTAAAAAAGAATTAACATGTACAG  >  CP000730/1302842‑1303102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑