New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 408323 =77 (1.210)3 (0.050) 3/260 10.2 3.8% coding (1869/2229 nt) metE 5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine S‑methyltransferase
?CP000730 408388 = 78 (1.310)coding (1804/2229 nt) metE 5‑methyltetrahydropteroyltriglutamate‑‑ homocysteine S‑methyltransferase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TATTAGCACTTGAAGCTGCAGGAATCAAAGTTATCCAAGTTGACGAACCTGCATTACGTGAAGGCTTACCATTACGCTCTGAATATCACGAACAATATCTTAAAGATGCTGTTTTATCATTTAAACTTGCAACGTCTTCAGTTCGTGAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/408471‑408323
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gttcgtgatATTCAGAGCGTAATGGTAAGCCTTCACGTAATGCAGGTTCGTCAACTTGGATAACTTTGATTCCTGCAGCTTCAAGTGCTAATACTTCTTCGTTGATTGCTAAAGCAATTTGATCTTGAACGACTTTACGTGGTAAAT  >  CP000730/408388‑408525
                                                                                                                                                                                                                                                                                               
TATTAGCACTTGAAGCTGCAGGAATCAAAGTTATCCAAGTTGACGAACCTGCATTACGTGAAGGCTTACCATTACGCTCTGAATATCACGAACAATATCTTAAAGATGCTGTTTTATCATTTAAACTTGCAACGTCTTCAG                                                                                                                                                    >  2:35117/1‑141
  TTAGCACTTGAAGCTGCAGGAATCAAAGTTATCCAAGTTGACGAACCTGCATTACGTGAAGGCTTACCATTACGCTCTGAATATCACGAACAATATCTTAAAGATGCTGTTTTATCATTTAAACTTGCAACGTCTTCAGTT                                                                                                                                                  >  2:618219/1‑141
  TTAGCACTTGAAGCTGCAGGAATCAAAGTTATCCAAGTTGACGAACCTGCATTACGTGAAGGCTTACCATTACGCTCTGAATATCACGAACAATATCTTAAAGATGCTGTTTTATCATTTAAACTTGCAACGTCTTCAGTT                                                                                                                                                  >  2:152195/1‑141
  TTAGCACTTGAAGCTGCAGGAATCAAAGTTATCCAAGTTGACGAACCTGCATTACGTGAAGGCTTACCATTACGCTCTGAATATCACGAACAATATCTTAAAGATGCTGTTTTATCATTTAAACTTGCAACGTCTTCAGTT                                                                                                                                                  >  2:618188/1‑141
   TAGCAGTTGAAGCTGCAGGAATCAAAGTTATCCAAGTGGACGAACCTGCATTACCTGAAGGCTTACCATTACGCTCTGAATATCACGAACAATATCTTAAAGATGCTGTTTTATCATTTAAACTTGCAACGTCTTCAGTTC                                                                                                                                                 <  1:492289/141‑1
   TAGCACTTGAAGCTGCAGGAATCAAAGTTATCCAAGTTGACGAACCTGCATTACGTGAAGGCTTACCATTACGCTCTGAATATCACGAACAATATCTTAAAGATGCTGTTTTATCATTTAAACTTGCAACGTCTTCAGTTC                                                                                                                                                 <  2:416304/141‑1
        CTTGAAGCTGCAGGAATCAAAGTTATCCAAGTTGACGAACCTGCATTACGTGAAGGCTTACCATTACGCTCTGAATATCACGAACAATATCTTAAAGATGCTGTTTTATCATTTAAACTTGCAACGTCTTCAGTTCGTGAT                                                                                                                                            >  1:80372/1‑141
                                                                                      CACGAACAATATCTTAAAGATGCTGTTTTATCATTTAAACTTGCAACGTCTTCAGTTCGTGATATTCAGAGCGTAATGGTAAGCCTTCACGTAATGCAGGTTCGTCAACTTGGATAACTTTGATTCCTGCAGCTTCAAGTG                                                              >  2:186075/1‑141
                                                                                             AATATCTTAAAGATGCTGTTTTATCATTTAAACTTGCAACGTCTTCAGTTCGTGATATTCAGAGCGTAATGGTAAGCCTTCACGTAATGCAGGTTCGTCAACTTGGATAACTTTGATTCCTGCAGCTTCAAGTGCTAATAC                                                       >  1:116926/1‑141
                                                                                                                                      TCTTCAGTTCGTGATATTCAGAGCGTAATGGTAAGCCTTCACGTAATGCAGGTTCGTCAACTTGGATAACTTTGATTCCTGCAGCTTCAAGTGCTAATACTTCTTCGTTGATTGCTAAAGCAATTTGATCTTGAACGACTT              <  2:116926/141‑1
                                                                                                                                            GTTCGTGATATTCAGAGCGTAATGGTAAGCCTTCACGTAATGCAGGTTCGTCAACTTGGATAACTTTGATTCCTGCAGCTTCAAGTGCTAATACTTCTTCGTTGATTGCTAAAGCAATTTGATCTTGAACGACTTTACGTG        >  2:443941/1‑141
                                                                                                                                            GTTCGTGATATTCAGAGCGTAATGGTAAGCCTTCACGTAATGCAGGTTCGTCAACTTGGATAACTTTGATTCCTGCAGCTTCAAGTGCTAATACTTCTTCGTTGATTGCTAAAGCAATTTGATCTTGAACGACTTTACGTG        >  1:95128/1‑141
                                                                                                                                                  GATATTCAGAGCGTAATGGTAAGCCTTCACGTAATGCAGGTTCGTCAACTTGGATAACTTTGATTCCTGCAGCTTCAAGTGCTAATACTTCTTCGTTGATTGCTAAAGCAATTTGATAATGCAAGAATTTACGTGGTAAAT  <  2:482021/141‑1
                                                                                                                                                                                                                                                                                               
TATTAGCACTTGAAGCTGCAGGAATCAAAGTTATCCAAGTTGACGAACCTGCATTACGTGAAGGCTTACCATTACGCTCTGAATATCACGAACAATATCTTAAAGATGCTGTTTTATCATTTAAACTTGCAACGTCTTCAGTTCGTGAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/408471‑408323
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gttcgtgatATTCAGAGCGTAATGGTAAGCCTTCACGTAATGCAGGTTCGTCAACTTGGATAACTTTGATTCCTGCAGCTTCAAGTGCTAATACTTCTTCGTTGATTGCTAAAGCAATTTGATCTTGAACGACTTTACGTGGTAAAT  >  CP000730/408388‑408525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.