New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 52489 =94 (1.050)38 (0.480) 4/246 14.2 30.3% intergenic (+99/‑574) USA300HOU_0043/USA300HOU_0044 hypothetical membrane protein/possible transcriptional regulator
?CP000730 = 52983 91 (1.140)intergenic (+593/‑80) USA300HOU_0043/USA300HOU_0044 hypothetical membrane protein/possible transcriptional regulator
Rejected: Coverage evenness skew score above cutoff.

ACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/52643‑52489
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tatttcacgcgatttTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGA  <  CP000730/52983‑52844
                                                                                                                                                                                                                                                                                                       
ACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATT                                                                                                                                                            <  1:816491/141‑1
  TACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTAT                                                                                                                                                          <  1:55667/141‑1
  TACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTAGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTAT                                                                                                                                                          >  1:930114/1‑141
   ACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATT                                                                                                                                                         >  1:501073/1‑141
       TGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCAC                                                                                                                                                     <  2:750128/141‑1
           TTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTTTTACTATGTTTTCAAACGTTTCCTCTGATTATTTCACGCtt                                                                                                                                                 >  2:932145/1‑139
            TCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCTCGCGATTACTACGATGATTTCAAACAATTCCTCTGATTATTTCACtagat                                                                                                                                                >  2:586872/1‑136
              AAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTT                                                                                                                                              >  2:543368/1‑141
                        TCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCACCATTACTACGATTTTTTCAAACGTTTCATCTGATTATTTCACGCGATTTTtactcaatt                                                                                                                                    >  2:927662/1‑132
                                  TCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAGCGATTCCTCTGATTATTTCACGCTATTTTCGCACGATTtttcttatgt                                                                                                                          >  2:791866/1‑131
                                               GCGCGATTACTACGATGATTTCAAACGATTCCTCTGGTTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTAT                                                                                                                                                          <  1:409318/96‑1
                                               GCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTAT                                                                                                                                                          >  2:409318/1‑96
                                                   GATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCA                                                                                                                                                      <  2:878163/96‑1
                                                   GATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCA                                                                                                                                                      >  1:878163/1‑96
                                                    ATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCA                                                                                                                                                      >  1:526131/1‑95
                                                    ATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCA                                                                                                                                                      <  2:526131/95‑1
                                                        CTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCAC                                                                                                                                                     >  2:73827/1‑92
                                                        CTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCAC                                                                                                                                                     <  1:73827/92‑1
                                                         TACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTGTCGCGCGATCACTACGATGATTTCAAACGATTCCTCTGATTAT                                                                                                   >  2:55667/1‑141
                                                              TGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTAT                                                                                                                                                          <  1:909071/81‑1
                                                              TGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTAT                                                                                                                                                          >  2:909071/1‑81
                                                                    CAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCACGATTACTCCGATTTCTTCAAACTATTACTCTCATTATTTCACGttatt                                                                                        >  1:380936/1‑136
                                                                              CTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCAC                                                                                                                                                     <  1:303121/70‑1
                                                                                  GATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCACGATTACTACGATGTTTTCAAACGAAACCTCTCATTATCCCACCCGATTTTtacaacattttt                                                                          >  2:647279/1‑129
                                                                                               gacTTTCGCGCGATTACTACGATCATATCAAACGTTCCCCCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAA                                                             <  2:61834/138‑1
                                                                                               GATTTTCGCGCGATTACTTCGATTTTCTCAAACCATCCCACTTATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAA                                                             <  2:532639/141‑1
                                                                                                 TTTTCGAGCGACTACTTCGATGTTCTCAAACGATTCCTCTTATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTTCGATGATTTCAAAC                                                           <  2:476969/141‑1
                                                                                                   TTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGA                                                         <  1:364955/141‑1
                                                                                                    TCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACG                                                                                             >  1:862615/1‑104
                                                                                                    TCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACG                                                                                             <  2:862615/104‑1
                                                                                                      GCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGAT                                                                     <  1:580951/126‑1
                                                                                                      GCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGAT                                                                     >  2:580951/1‑126
                                                                                                      GCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGAT                                                                                                                            >  2:785769/1‑71
                                                                                                      GCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCG                                                                                                                                         >  1:262150/1‑58
                                                                                                      GCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCG                                                                                                                                           >  1:268297/1‑56
                                                                                                       CGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCT                                                                                                         <  2:44160/89‑1
                                                                                                       CGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCT                                                                                                         >  1:44160/1‑89
                                                                                                        gttATTACAACGATGTTCTCAAACCATCCCTCTTATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCT                                                    <  2:705213/138‑1
                                                                                                        GCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGGTTTCAAACGATTCCTCTG                                                                                                        <  2:961229/89‑1
                                                                                                        GCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGA                                                                                                       >  2:602753/1‑90
                                                                                                        GCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTG                                                                                                        >  1:961229/1‑89
                                                                                                        GCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATT                                                                                                              >  1:521854/1‑83
                                                                                                        GCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATT                                                                                                              <  2:521854/83‑1
                                                                                                         ctATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTC                                                   <  1:311555/139‑1
                                                                                                          GATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCT                                                  <  1:959486/141‑1
                                                                                                                TACGATTTTCTCAAACCATTCCACTTATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTAT                                            <  2:379269/141‑1
                                                                                                                 ACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATT                                           <  1:786255/141‑1
                                                                                                                   GATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAA                                                                                                                    >  1:613196/1‑66
                                                                                                                    ATGATTTCAAACGGTTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCA                                        <  1:850095/141‑1
                                                                                                                     TGATTTCAAACGATTTCTCTTATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCAC                                       <  1:789510/141‑1
                                                                                                                      tATTTCAAACCATCCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACG                                      <  2:377475/140‑1
                                                                                                                      GATTTCAAACGATCCCTCTGATTATTTCACGCGATTTTCGCGCGATTTCTACTTGGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACG                                      <  1:866648/141‑1
                                                                                                                        TTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACG                                                                                             >  1:605287/1‑84
                                                                                                                        TTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACG                                                                                             <  2:605287/84‑1
                                                                                                                              ACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAAC                                                                                                                  <  2:509850/57‑1
                                                                                                                              ACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAAC                                                                                                                  >  1:509850/1‑57
                                                                                                                                     CTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCAC                                                                                              >  2:303121/1‑70
                                                                                                                                              TTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGAT                                                                     >  2:884214/1‑86
                                                                                                                                              TTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGAT                                                                     <  1:884214/86‑1
                                                                                                                                              TTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCG                                                                                           >  2:318033/1‑64
                                                                                                                                              TTTCACGCGATTTTCGCGCGATTACTACGATGATTTCA                                                                                                                     >  1:936788/1‑38
                                                                                                                                               TTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATG             >  1:561333/1‑141
                                                                                                                                                 CACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGAT           <  1:59054/141‑1
                                                                                                                                                   CGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCC                                                                                                            >  1:107436/1‑42
                                                                                                                                                    GCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTC        <  1:897982/141‑1
                                                                                                                                                     CGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCGTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCA       >  1:614602/1‑141
                                                                                                                                                     CGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCA       <  1:901279/141‑1
                                                                                                                                                          TTCGCGCGATTACAACGATGATTTCAAACCATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGA  <  2:748288/141‑1
                                                                                                                                                                                                                                                                                                       
ACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGTGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP000730/52643‑52489
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tatttcacgcgatttTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGATTCCTCTGATTATTTCACGCGATTTTCGCGCGATTACTACGATGATTTCAAACGA  <  CP000730/52983‑52844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.