New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 811555 =42 (1.100)4 (0.110) 3/262 6.2 11.8% intergenic (+160/‑117) USA300HOU_0757/USA300HOU_0758 hypothetical protein/hypothetical protein
?CP000730 = 891954 20 (0.550)intergenic (+21/‑370) USA300HOU_0844/yusF hypothetical protein/TOPRIM domain topiosmerase/primase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AAGCTTGTTACAAGCGCATTTTCGTTCAGTCAACTACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCAGCCTGTTTTCATAAGCCACAGCTAATGTGTATTTAAAAATAGGAATACATGAGTAAAACTCATGCATAAGAAATACTAATTTCTAAAGAAAAAGTATTTCTTTATGTTGGGGCCCCGCCAACTTGCACATTATTGTAAGCTGACTTACCATCAGCTTCTGTGTTGGGGCCCCGCTATAATTG  >  CP000730/891816‑892079
                                                                                                                                          |                                                                                                                             
aaGCTTGTTACAAGCGCATTTTCGTTCAGTCAACTACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCAGCCTGTTTTCATAAGCCACAGCTAATGTGTATTTAAAAATAGGAATACATGAGTAAAAc                                                                                                                             >  2:170238/1‑141 (MQ=255)
     tGTTACAAGCGCATTTTCGTTCAGTCAACTACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCAGCCTGTTTTCATAAGCCACAGCTAATGTGTATTTAAAAATAGGAATACATGAGTaaaagatcgg                                                                                                                        >  1:77627/1‑135 (MQ=255)
     agTTACAAGCGCATTTTCGTTCAGTCAACTACTGCCAATATAACTTCGTAGAGCATAGCACATTGATTTATGTCCCAGCCTGTTTTCATACGCCACAGCTAATGTGTATTTAAAAATAGGAATACATGAGGAAAACTCATg                                                                                                                        >  2:130559/2‑141 (MQ=255)
                   tttCGTTCAGTCAACTACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCAGCCTGTTTTCATAAGCCACAGCTAATGTGTATTTAAAAATAGGAATACATGAGTAAAACTCATGCATAAGAAATACTa                                                                                                          >  2:357755/1‑141 (MQ=255)
                    ttCGTTCAGTCAACTACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCAGCCTGTTTTCATAAGCCACAGCTAATGTGTATTTAAAAATAGGAATACATGAGTAAAACTCATGCATAAGAAATACTaa                                                                                                         >  2:348391/1‑141 (MQ=255)
                                actactGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCAGCCTGTTTTCATAAGCCACAGCTAATGTGTATTTAAAAATAGGAATACATGAGTAAAACTCATGCATAAGAAATACTAATTTCTAAAGaaa                                                                                             >  1:249254/1‑141 (MQ=255)
                                              aaCGTCGTAGAGCATCCAACATTAACTTAGGTCCCAGCCTTTTTTCATAAGCCACAGCTAATGTGTATTTAAAAATAGGAATACATGAATAAAACTCATGCATAAGAAATACTAATTTCTAAAGAAAAAGTATTTCTTTAt                                                                               >  2:75744/1‑141 (MQ=18)
                                                cTTCGTAGAGCATAGAACATTGATTTATGTCCCAGCCTGTTTTCATAAGCCACAGCTAATGTGTATTTAAAAATAGGAATACATGAGTAAAACTCATGCATAAGAAATACTAATTTCTAAAGAAAAAGTATTTCTTTATGt                                                                             >  1:77219/1‑141 (MQ=255)
                                                     tAGAGCATAGAACATTGATTTATGTCCCAGCCTGTTTTCATAAGCCACAGCTAATGTGTATTTAAAAATAGGAATACATGAGTAAAACTCATGCATAAGAAATACTAATTTCTAAAGAAAAAGTATTTCTTTATGTTgggg                                                                        <  1:160338/141‑1 (MQ=255)
                                                     tAGAGCATAGAACATTGATTTATGTCCCAGCCTGTTTTCATAAGCCACAGCTAATGTGTATTTAAAAATAGGAATACATGAGTAAAACTCATGCATAAGAAATACTAATTTCTAAAGAAAAAGTATTTCTTTATGTTgggg                                                                        <  1:51491/141‑1 (MQ=255)
                                                         gCATAGAACATTGATTTATGTCCCAGCCTGTTTTTATTTTTTATAGCTAATGTGTATATAAAAATAGGAATACATGAGTAAAACTCATGCATAAGAAATACTAATTTCTAAAGAAAAAGTATATCTTTATGtaaggtaaca                                                                    >  2:185927/1‑132 (MQ=17)
                                                                                          tCATAAGCCACAGCTAATGTGTATTTAAAAATAGGAATACATGAGTAAAACTCATGCATAAGAAATACTAATTTCTAAAGAAAAAGTATTTCTTTATGTTGGGGCCCCGCCAACTTGCACATTATTGTAAGCTGACTTAcc                                   >  2:193161/1‑141 (MQ=255)
                                                                                           cATAAGCCACAGCTAATGTGTATTTAAAAATAGGAATACATGAGTAAAACTCATGCATAAGAAATACTAATTTCTAAAGAAAAAGTATTTCTTTATGTTGGGGCCCCGCCAACTTGCACATTATTGTAAGCTGACTTACCa                                  <  1:318282/141‑1 (MQ=255)
                                                                                                gCCACAGCTAATGTGTATTTAAAAATAGGAATACATGAGTAAAACTCATGCATAAGAAATACTAATTTCTAAAGAAAAAGTATTTCTTTATGTTGGGGCCCCGCCAACTTGCACATTATTGTAAGCTGACTTACCATCAGc                             >  2:38972/1‑141 (MQ=255)
                                                                                                 ccaccgttcaTGTGTATTTAAAAATAGGAATACATGAGTAAAACTCATGCATAAGAAATTCTAATTTCTAAAGAAAAAGTATTTCTTTATGTTTGGGTCCCTCCAACTTGCACATTATTATAAGCTTACTTTCCATCAGCt                            <  2:392578/132‑1 (MQ=17)
                                                                                                  cacaGCTAATGTGTATTTAAAAATAGGAATACATGAGTAAAACTCATGCATAAGAAATACTAATTTCTAAAGAAAAAGTATTTCTTTATGTTGGGGCCCCGCCAACTTGCACATTATTGTAAGCTGACTTACCATCAGCtt                           >  1:66298/1‑141 (MQ=255)
                                                                                                   acaGCTAATGTGTATTTAAAAATAGGAATACATGAGTAAAACTCATGCATAAGAAATACTAATTTCTAAAGAAAAAGTATTTCTTTATGTTGGGGCCCCGCCAACTTGCACATTATTGTAAGCTGACTTACCATCAGCTTc                          >  2:311589/1‑141 (MQ=255)
                                                                                                        tAATGTGTAGTTAAAAATAGGAATACATGAGTAAAACTCATGCATAAGAAATACTAATTTCTAAAGAAAAAGTATTTCTTTATGTTGGGGCCCCGCCAACTTGCACATTATTGTAAGCTGACTTACCATCAGCTTCTgtgt                     <  2:171427/141‑1 (MQ=255)
                                                                                                                    aaaaaTAGGAATACATGAGTAAAACTCATGCATAAGAAATACTAATTTCTAAAGAAAAAGTATTTCTTTATGTTGGGGCCCCGCCAACTTGCACATTATTGTAAGCTGACTTACCATCAGCTTCTGTGTTGGGGCCCCGCt         <  1:164909/141‑1 (MQ=255)
                                                                                                                     aaaaTAGGAATACATGAGTAAAACTCATGCATAAGAAATACTAATTTCTAAAGAAAAAGTATTTCTTTATGTTGGGGCCCCGCCAACTTGCACATTATTGTAAGCTGACTTACCATCAGCTTCTGTGTTGGGGCCCCGCta        <  1:170238/141‑1 (MQ=255)
                                                                                                                     aaaaTAGGAATACATGAGTAAAACTCATGCATAAGAAATACTAATTTCTAAAGAAAAAGTATTTCTTTATGTTGGGGCCCCGCCAACTTGCACATTATTGTAAGCTGACTTACCATCAGCTTCTGTGTTGGGGCCCCGCta        >  1:61440/1‑141 (MQ=255)
                                                                                                                          aGGAATACATGAGTAAAACTCATGCATAAGAAATACTAATTTCTAAAGAAAAAGTATTTCTTTATGTTGGGGCCCCGCCAACTTGCACATTATTGTAAGCTGACTTACCATCAGCTTCTGTGTTGGGGCCCCGCTATAAtt   >  2:211952/1‑141 (MQ=255)
                                                                                                                           ggAATACATGAGTAAAACTCATGCATAAGAAATACTAATTTCTAAAGAAAAAGTATTTCTTTATGTCGGGGCCCCGCCAACTTGCACATTATTGTAAGCTGACTTACCATCAGCTTCTGTGTTGGGGCCCCGCTATAATTg  >  1:332098/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                             
AAGCTTGTTACAAGCGCATTTTCGTTCAGTCAACTACTGCCAATATAACTTCGTAGAGCATAGAACATTGATTTATGTCCCAGCCTGTTTTCATAAGCCACAGCTAATGTGTATTTAAAAATAGGAATACATGAGTAAAACTCATGCATAAGAAATACTAATTTCTAAAGAAAAAGTATTTCTTTATGTTGGGGCCCCGCCAACTTGCACATTATTGTAAGCTGACTTACCATCAGCTTCTGTGTTGGGGCCCCGCTATAATTG  >  CP000730/891816‑892079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.