New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	68659 =	26 (0.760)	3 (0.100)	3/240	5.1	11.9%	coding (775/852 nt)	USA300HOU_0065	transposase
	?	CP000730	= 68655	22 (0.740)					coding (771/852 nt)	USA300HOU_0065	transposase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AGGGAACACCTGCCGATAACGCCCCGATAGAAAGTTTCCATTCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTAAGGGTTCAGTGCCCTAGGATTATAGGCTCTT  >  CP000730/68522‑68798                                                                                                                                          |                                                                                                                                            aGGGAACACCTGCCGATAACGCCCCGATAGAAAGTTTCCATTCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACaataata                                                                                                                                          <  1:199174/141‑1 (MQ=255)      acacCTGCCGATAACGCCCCGATAGAAAGTTTCCATTCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCAAATTATAATAGATATTGTCGAAAAGTACATTAAAAACaataataataat                                                                                                                                     <  2:352007/141‑1 (MQ=255)               gATAACGCCCCGATAGAAAGTTTCCATTCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACaataataataataataataat                                                                                                                            <  1:369972/141‑1 (MQ=255)                          gATAGAAAGTTTCCATTCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTcaaca                                                                                                                 <  1:290545/141‑1 (MQ=255)                           aTAGAAAGTTTCCATTCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTcaacaa                                                                                                                <  2:327417/141‑1 (MQ=255)                                   tttCCATTCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAGTCACATAAGAATAAGTATTTTCGAAAATTACATTAAAAACAATAAGAATAATAATAATAATCGAATTCAACAAAAACTAgg                                                                                                        >  1:34771/1‑141 (MQ=21)                                   tttCCATCCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAgg                                                                                                        <  2:290815/141‑1 (MQ=255)                                       cATTCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTAc                                                                                                    >  2:44182/1‑141 (MQ=255)                                          tCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACAGTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTa                                                                                                 <  1:360954/141‑1 (MQ=255)                                               gatAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAATACATTAAAAACAATAATAATAATAATAATAATCGAAAACAACAAAAACTAGGCTACTTATcccc                                                                                            <  2:63618/139‑1 (MQ=255)                                                 tAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCTCCTg                                                                                          >  1:109782/1‑141 (MQ=255)                                                  aaaGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGt                                                                                         >  1:213618/1‑141 (MQ=255)                                                     gTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAAAAGAAAACAAAAAAAACTAGGCTACTTATCCCCTGTaaa                                                                                      <  2:278632/141‑1 (MQ=21)                                                       cTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAAt                                                                                    >  1:364667/1‑141 (MQ=255)                                                                   aCATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAAC‑‑‑AATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAg                                                                     >  1:13350/1‑141 (MQ=255)                                                                                gCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACAt                                                           >  2:237009/1‑141 (MQ=255)                                                                                                  caTTGTAATAGATATTGTCGAAAAGTACATTAAAAAC‑‑‑AATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCg                                      >  1:249364/1‑141 (MQ=255)                                                                                                        aaTAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGATACTAATAAAACCTACAATAACGAGAATTAATAGTCTGGACGATGGAGACTTTATTAAGTTCACGttt                                   >  1:313076/1‑141 (MQ=255)                                                                                                         aTAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATTGTGTTTTTATTAAGTACCCGtttt                                  >  2:297624/1‑141 (MQ=255)                                                                                                           aGATATTGTCAAAAAGTACAATAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAATCTAGGCTACTTATCCCCGGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTaa                                <  2:233565/141‑1 (MQ=255)                                                                                                                ttGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTAAGGGtt                           >  1:2344/1‑141 (MQ=255)                                                                                                                  gTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTAAGGGTTCa                         >  2:358642/1‑141 (MQ=255)                                                                                                                      aaaaGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTAAGGGTTCAGTGc                     <  1:303455/141‑1 (MQ=255)                                                                                                                      aaaaGTACATTAAAAAC‑‑‑AATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTAAGGGTTCAGTGCCCt                  <  2:294728/141‑1 (MQ=255)                                                                                                                         aGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTAAGGGTTCAGTGCCCt                  >  1:22542/1‑141 (MQ=255)                                                                                                                           tACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTAAGGGTTCAGTGCCCTAg                >  1:540/1‑141 (MQ=255)                                                                                                                            aCATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTAAGGGTTCAGTGCCCTAgg               <  2:320422/141‑1 (MQ=255)                                                                                                                                         taataataataataataatCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTAAGGGTTCAGTGCCCTAGGATTATAGGCTCtt  >  1:369398/1‑141 (MQ=255)                                                                                                                                         taaaaagaataataataatCGAATTCAACAAAAACTAGGCTACATATCCCCTGTAACATACAGAGAATCAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTAAGGGTTCAGTGCCCTAGGATTATAGGCTCtt  >  2:305643/1‑141 (MQ=17)                                                                                                                                          |                                                                                                                                            AGGGAACACCTGCCGATAACGCCCCGATAGAAAGTTTCCATTCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTAAGGGTTCAGTGCCCTAGGATTATAGGCTCTT  >  CP000730/68522‑68798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.