New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 68659 =26 (0.760)3 (0.100) 3/240 5.1 11.9% coding (775/852 nt) USA300HOU_0065 transposase
?CP000730 = 68655 22 (0.740)coding (771/852 nt) USA300HOU_0065 transposase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CCCGAAAGGGAACACCTGCCGATAACGCCCCGATAGAAAGTTTCCATTCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTAAGGGTTCAGTGCCCTAGG  >  CP000730/68516‑68785
                                                                                                                                           |                                                                                                                                  
cccGAAAGGGAACACCTGCCGATAACGCCCCGATAGAAAGTTTCCATTCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACa                                                                                                                                   <  1:315794/141‑1 (MQ=255)
      aGGGAACACCTGCCGATAACGCCCCGATAGAAAGTTTCCATTCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACaataata                                                                                                                             <  1:199174/141‑1 (MQ=255)
           acacCTGCCGATAACGCCCCGATAGAAAGTTTCCATTCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCAAATTATAATAGATATTGTCGAAAAGTACATTAAAAACaataataataat                                                                                                                        <  2:352007/141‑1 (MQ=255)
                    gATAACGCCCCGATAGAAAGTTTCCATTCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACaataataataataataataat                                                                                                               <  1:369972/141‑1 (MQ=255)
                               gATAGAAAGTTTCCATTCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTcaaca                                                                                                    <  1:290545/141‑1 (MQ=255)
                                aTAGAAAGTTTCCATTCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTcaacaa                                                                                                   <  2:327417/141‑1 (MQ=255)
                                        tttCCATTCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAGTCACATAAGAATAAGTATTTTCGAAAATTACATTAAAAACAATAAGAATAATAATAATAATCGAATTCAACAAAAACTAgg                                                                                           >  1:34771/1‑141 (MQ=21)
                                        tttCCATCCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAgg                                                                                           <  2:290815/141‑1 (MQ=255)
                                            cATTCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTAc                                                                                       >  2:44182/1‑141 (MQ=255)
                                               tCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACAGTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTa                                                                                    <  1:360954/141‑1 (MQ=255)
                                                    gatAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAATACATTAAAAACAATAATAATAATAATAATAATCGAAAACAACAAAAACTAGGCTACTTATcccc                                                                               <  2:63618/139‑1 (MQ=255)
                                                      tAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCTCCTg                                                                             >  1:109782/1‑141 (MQ=255)
                                                       aaaGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGt                                                                            >  1:213618/1‑141 (MQ=255)
                                                          gTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAAAAGAAAACAAAAAAAACTAGGCTACTTATCCCCTGTaaa                                                                         <  2:278632/141‑1 (MQ=21)
                                                            cTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAAt                                                                       >  1:364667/1‑141 (MQ=255)
                                                                        aCATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAAC‑‑‑AATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAg                                                        >  1:13350/1‑141 (MQ=255)
                                                                                     gCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACAt                                              >  2:237009/1‑141 (MQ=255)
                                                                                                       caTTGTAATAGATATTGTCGAAAAGTACATTAAAAAC‑‑‑AATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCg                         >  1:249364/1‑141 (MQ=255)
                                                                                                             aaTAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGATACTAATAAAACCTACAATAACGAGAATTAATAGTCTGGACGATGGAGACTTTATTAAGTTCACGttt                      >  1:313076/1‑141 (MQ=255)
                                                                                                              aTAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATTGTGTTTTTATTAAGTACCCGtttt                     >  2:297624/1‑141 (MQ=255)
                                                                                                                aGATATTGTCAAAAAGTACAATAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAATCTAGGCTACTTATCCCCGGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTaa                   <  2:233565/141‑1 (MQ=255)
                                                                                                                     ttGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTAAGGGtt              >  1:2344/1‑141 (MQ=255)
                                                                                                                       gTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTAAGGGTTCa            >  2:358642/1‑141 (MQ=255)
                                                                                                                           aaaaGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTAAGGGTTCAGTGc        <  1:303455/141‑1 (MQ=255)
                                                                                                                           aaaaGTACATTAAAAAC‑‑‑AATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTAAGGGTTCAGTGCCCt     <  2:294728/141‑1 (MQ=255)
                                                                                                                              aGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTAAGGGTTCAGTGCCCt     >  1:22542/1‑141 (MQ=255)
                                                                                                                                tACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTAAGGGTTCAGTGCCCTAg   >  1:540/1‑141 (MQ=255)
                                                                                                                                 aCATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTAAGGGTTCAGTGCCCTAgg  <  2:320422/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                  
CCCGAAAGGGAACACCTGCCGATAACGCCCCGATAGAAAGTTTCCATTCCTCGCTAAAGTCTGAAACTTTTTACATCAATAATGAGCTTAATCACTCTAATCACATTGTAATAGATATTGTCGAAAAGTACATTAAAAACAATAATAATAATAATAATAATCGAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAAATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTAAGGGTTCAGTGCCCTAGG  >  CP000730/68516‑68785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.